ISOLATION AND IDENTIFICATION OF MYCOFLORA FROM MAIZE SEEDS AND THEIR IMPACTS ON PROTEIN QUALITY
DOI:
https://doi.org/10.29121/shodhkosh.v5.i6.2024.5553Keywords:
Maise ( Zea Mays L .), Mycoflora, Aflatoxin B1, Amino Acids KitsAbstract [English]
Maize is one of the major food crops around the world and it provides energy for human and animals. It grows throughout the year due to its photothermo insensitive character and highest genetic yield. The composition of maize contents are Moisture 14.9% , Fat 3.6% , Fibre 2.7 % , Minerals 1.5 % and Protein 11.5% respectively. The economic value of the protein contents are utilized in the production of food products .The various genera of fungi like .Aspergillus, Penicillium , Alternaria, Fusarium and Rhizopus were recorded from maize sample of Var. A and Var. B . The dominant fungi A. flavus (760 which produce toxic substance like Aflatoxin B1 . Aflatoxin B1 known to adverse the seed germination and decline the quality ( Protein ) contents . The inhibition of seed germination were maximum 74.67% and 75.67% in both the Var. A and Var B. at 2000 ppb concentration of aflatoxin B1 respectivily , whereas the maximum inhibition of protein contents ( 73.34% and 49.36 % ) in Var. A and Var. B were also recored at 2000ppb concentration of aflatoxin B1 ,respectively
Therefore , The healthy maize seeds should be released to the farmers in order to the secure their outputs as well as to provide the quality of energetic food for the human beings.
References
Bewley, J.D. Bradfrord, K., Hilhorst, H., & Nonogaki, H. (2013). Seess: Physiology of development, germination and dormancy. Spinger Science & Business Media. DOI: https://doi.org/10.1007/978-1-4614-4693-4
Bathla S, Jaidka M, Kaur R.2019. Nutritive value. In (eds.) Maize production and use. Intechopen.http://doi.org/10.5772/intechaper open.88963. DOI: https://doi.org/10.5772/intechopen.88963
Choudhary, A.K., Sharma, R., & Kumari, P. (2008), Effect of aflatoxin B1 on seed germination, seedling growth and some biochemical parameters of Glycine max L, American- Eurasion Journal of Botany, 1(2), 67-71.
Dospekov, B.A. (1984). Field experimentation, statistical procedures (V.P. Kolykhmatov, Ed., Vol. 352). Mir Publishers.
Erenstein, O, Jaleta, M, Sonder, K ; Mottaleb, K, Prasunna, B.M. Global maize production, Consumption and trade, Trends and R & D implications, Food secur, 2022 ,14 ,1294-1319. DOI: https://doi.org/10.1007/s12571-022-01288-7
G.C. Andrade et.al., Modelling the vigour of maize seeds submitted to artificial accelerated ageing based on ATR-FTIR data and chemometic tools (PCA, HCA and PLS-DA) - (Heliyon-2020). DOI: https://doi.org/10.1016/j.heliyon.2020.e03477
Kachapulula, P.W., Akello, J., Bandyapadhay, R., COTTY, P.J. (2017). Aflatoxin contamination of groundnut &maize in zambia. Observed and potential concentrations. Journal of Applied Microbiology, 122(6),1471-1482. DOI: https://doi.org/10.1111/jam.13448
Dudoiu R, Cristea S, Lupu C, et.al., (2016). Microflora associated with maize grains during storage period, Agrolife scientific Journal 5(1): 63-58.
Huang, Z., Zhao. L., Chen, D., Liang, M., Liu, Z., Show, H., …&Cjhemn, H. (2013). The role of proteins in seed germination. Plant signaling & Behaviour,8(2), e 24172.
Lowery, O.H., Rosenbrough, N. J., Farr, A.L., &Randall, R.J. (1951). Protein measurement with the folin –phenol reagent. Journal of Biological Chemistry, 193, 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Tanumihardjo, S., McCulley, L., Roh, R., Lopez-Ridaura, S., Palacios-Rojas, N., & Gunaratna, N. (2019). Maize agro – food systems to ensure food and nutrition security in reference to the Sustainable Development Goals. Global Food Security, 25, 100327. https://doi.org/10.1016/j.gfs.2019.100327. DOI: https://doi.org/10.1016/j.gfs.2019.100327
Prasad, G. (1997). Combined effects of aflatoxin B 1 and citrin on maize seedling. Biologia plantarum. 40, 441-447. DOI: https://doi.org/10.1023/A:1001182401477
Prasad, G. & N. Kumari (2018). Efficacy of GA3 on amino acid contents in aflatoxin B1 treated maize seeds. Bioglobia, 5 (1), 26-29.
Sinha, K.K., and Kumari, P. 1990. Some physiological abnormalties induced by aflatoxin (AFTB1) in mung seeds (Vigna Radiata var. Pusa Baishakhi). Mycopathologia, 110: 77-79. DOI: https://doi.org/10.1007/BF00446994
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