EFFECT OF L-929 DERIVED SECRETOME ON HUH-7 HEPATIC CANCER CELL- ON CELLULAR PROLIFERATION AND OXIDATIVE STRESS

Virbhan Singh; Mridul Sharma; Seemha Rai

doi:10.29121/shodhkosh.v5.i6.2024.4946

Authors

Virbhan Singh Center for Stem Cell Tissue Engineering and Biomedical Excellence, Panjab University, Chandigarh
Mridul Sharma Center for Stem Cell Tissue Engineering and Biomedical Excellence, Panjab University, Chandigarh
Seemha Rai Center for Stem Cell Tissue Engineering and Biomedical Excellence, Panjab University, Chandigarh- 160014, India

DOI:

https://doi.org/10.29121/shodhkosh.v5.i6.2024.4946

Keywords:

L-929 Fibroblasts, Huh-7 Cells, Secretome, Tumor Microenvironment, Hepatocellular Carcinoma, Cellular Proliferation, Migration, Oxidative Stress

Abstract [English]

The tumor microenvironment (TME) plays a crucial role in cancer progression, where stromal components such as fibroblasts actively influence tumor cell behavior. This study investigates the effects of the secretome derived from L-929 fibroblasts on HuH-7 hepatic cancer cells on cellular proliferation and oxidative stress. The interaction between fibroblast-derived factors and hepatic carcinoma cells has not been extensively explored. Through in vitro assays, including MTT and ROS analysis, it was found that L-929 fibroblast secretome decreased the HuH-7 proliferation and also induced oxidative stress in HUH-7 cells. The findings contribute to a deeper understanding of tumor-stromal interactions and potential therapeutic implications for hepatocellular carcinoma (HCC).

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