EFFECTS OF LASER IRRADIATION ON NORMAL AND ANEMIC HUMAN BLOOD
DOI:
https://doi.org/10.29121/granthaalayah.v8.i8.2020.862Keywords:
Laser Irradiation, Normal And Anemic Human Blood, Optical Properties Of Human BloodAbstract [English]
The effects of laser irradiation on the whole human blood were studied. The blood samples were taken from healthy donors with normal blood and donors with anemic blood. The blood samples were exposed to laser radiation. Two lasers of the same types, continuous wave (CW) diode pumped solid-state lasers (DPSSL’ s) were used to irradiate the blood samples. One of these laser has a wavelength 532 nm (green laser beam), while the other laser has a wavelength 671 nm (red laser beam). The output power of the two lasers can be varied over the range 0 - 100 mW. In the present study, the output power was fixed at 20 mW for both lasers. The blood samples were exposed to different irradiation times, 5, 15, and 30 min. Significant changes in the optical parameters of the irradiated blood samples were observed in comparison with that of the un-irradiated blood samples (the control samples). These optical parameters were determined from the measured absorbance spectra of the un-irradiated and irradiated samples. The absorbance spectra were measured using a UV-Visible double-beam spectrophotometer operating over the wavelength range 190 - 1100 nm.
The results of the present study revealed that the laser irradiation can produce considerable effects on the human blood samples, suggesting the suitability of the laser beam irradiation for the biological and medical applications.
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References
A. N. Rubinov,"Physical Grounds for Biological Effect of Laser Radiation", J. Phys. D: Appl. Phys., 36.19 (2003) 2317-2330. DOI: https://doi.org/10.1088/0022-3727/36/19/002
S. Restegar, B. Kim and L. Jacques, ''Role of Temperature Dependence of Optical Properties in Laser Irradiation of Biological Tissue'', Proc. SPIE, Vol. 1646, (1992) 228-231. DOI: https://doi.org/10.1117/12.137464
M. S. Strikman, K. Spartalia, and M.W. Cole, Applications of Modern Physics in Medicine, (Princeton University Press, 2014).
M. H. Niemz, Laser - Tissue Interaction: Fundamentals and Applications (Biological, Medical, Physics, and Biomedical Engineering), 4th Edition (Springer, New York, USA, 2019).
G. R. Kulkarni, '' Laser Tissue Interaction Studies for Medicine '', Bull. Mater. Sci., 11 (1988) 239-244. DOI: https://doi.org/10.1007/BF02744557
E. Mester, A. F. Mester and A. Mester, The Biomedical Effects of Laser Application, Laser Surg. Med., 5 (1988) 31-39. DOI: https://doi.org/10.1002/lsm.1900050105
B. Azadgoli and R. V. Baker, "Laser Applications in Surgery" (Review Article on Innovations Technology in Surgery) Ann. Transl. Med., 4 (2016) 452-458. DOI: https://doi.org/10.21037/atm.2016.11.51
O. Svelto, Principles of Laser, 5th Edition, (Springer Science + Business Media LLC, New York, USA, 2010).
P. Di Giacomo, S. Oriando, M. Dell Ariccia, and B. Brandimare , '' Low Level Laser Therapy: Laser Radiation Absorption in Biological Tissues '', Appl. Phys., 112 (2013) 71-75. DOI: https://doi.org/10.1007/s00339-012-7204-z
S. L. Jacques, '' Optical Properties of Biological Tissues: A Review", Phys. Med. Bilog., 58 (2013) 37- 61. DOI: https://doi.org/10.1088/0031-9155/58/11/R37
J. Zdrojkowski and N. Pisharoty, '' Optical Transmission and Reflection by Blood '', IEEE Transactions on Biomedical Engineering, 17 (1970) 122-128. DOI: https://doi.org/10.1109/TBME.1970.4502710
V. H. Ghadage and B. N. Zaware, '' Study the Effect of He - Ne (He-Ne) Laser Irradiation on Normal Human Blood In vitro Using FTIR Technique'', IOSR, J. Pharmacy and Biological Sciences, 13 (2008)1-5.
H. Ma, Y. Yang, and S. Cherng, '' Interaction of Excimer Laser with Blood Components and Thrombosis '', Life Science (2008) 19-26.
G. A. Zaiesskaya, E. G. Sambor, ''Interaction of Low - intensity Laser Radiation with Blood and Components '', J. APPI – Sepectrosc., 72 (2005), 242-248. DOI: https://doi.org/10.1007/s10812-005-0062-0
W. F. Cheong, S. A. Prahl, and A. J. Welch, ''A Review of the Optical Properties of Biological Tissues", IEEE Quant. Electron., 26.12 (1990): 2166-2185. DOI: https://doi.org/10.1109/3.64354
J. D. Bronzino, and D. R. Peterson, (Ed.), (The Biomedical Engineering Handbook), 4th Edition (CRC Press, Florida, USA, 2015).
T. Halldorsson,'' Alteration of Optical Properties of Blood by Nd: YAG Laser Irradiation '', In Proc. 4th Cong. Int. Soc. Laser Surgery, Tokyo, Japan, Nov. (1981) 1-8. DOI: https://doi.org/10.1002/lsm.1900010309
J. K. Barton, D. P. Popok, and J.F. Black, ''Thermal Analysis of Blood Undergoing Laser Photocoagulation '', IEEE J. Quant. Electron., 7 (2001) 936- 943. DOI: https://doi.org/10.1109/2944.983297
J. Cai (Ed.), Atomic Force Microscopy in Molecular and Cell Biology, (Springer, New York, USA, 2018). DOI: https://doi.org/10.1007/978-981-13-1510-7
W. A. Hendrickso, "X -Rays in Molecular Biophysics", Physics Today, 48 (1995) 42-50. DOI: https://doi.org/10.1063/1.881481
K. Wilson, and J. Wlaker (Eds.), Principles and Techniques of Biochemistry and Molecular Biology, 8th Edition, (Cambridge University Press, Cambridge, UK, 2018).
Y. Cui, Z. Guo, Y. Zhao, Y. Zheng, Y. Qiao, J. Gai,and S. Liu, " Reactive Effect of Low Intensity He-Ne Laser Upon Damaged Ultra Structure of Human Erythrocyte Membrane in Fenton System by Atomic Force Microscope '' Acta Biochemica et Biophysica Sinica , 39 (2007) 484-489. DOI: https://doi.org/10.1111/j.1745-7270.2007.00309.x
T. Chelidze, '' Dielectric Spectroscopy of Blood '', J. Non - Crystalline Solids, 305 (2002) 285-294. DOI: https://doi.org/10.1016/S0022-3093(02)01101-8
M. Qiany, J. Chen, Z. Junliang and L. Welzhou, ''In Vitro Effects of He–Ne Laser Irradiation on Human Blood: Blood Viscosity and Deformability of Erythrocytes '', Photo Medicine and Laser Surgery, 22 (2004) 477- 482. DOI: https://doi.org/10.1089/pho.2004.22.477
R. Lubart, Y. Wollman, H. Friedmann, S. Rochkind, and I. Laulicht, '' Effects of Visible and Infrared Laser on Cell Culture," J. Photochem., Photobiolog., B, 12 (1992) 305- 310. DOI: https://doi.org/10.1016/1011-1344(92)85032-P
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