COMPARATIVE STUDY OF MARINE ALGAE ISOLATED FROM EAST COAST OF INDIA BY REDUCING POWER ASSAY

Leelavathi M S; Prasad M P

doi:10.29121/granthaalayah.v5.i4RAST.2017.3306

Authors

Leelavathi M S Research Scholar, Bharathiar University, Coimbatore, India Faculty, VidyaSoudha PU College, Peenya, Bangalore, India
Prasad M P Prasad M P, Senior Scientist, Sangenomics Research Labs, Bangalore, India

DOI:

https://doi.org/10.29121/granthaalayah.v5.i4RAST.2017.3306

Keywords:

Reducing Power Assay, Antioxidant, GracillariaSpecies, CymodeaceaSpecies, Ulvalactuca

Abstract [English]

The Gulf of Mannar is a Marine Biosphere Reserve situated along the east coast of India and Sri Lanka, an area of about 10,500 sq. km which has a luxuriant growth of about 680 species of seaweed belonging to the Rhodophyta, Pheaophyta and Chlorophyta, in both the inter-tidal and deep water regions. Seaweed constitutes a commercially important marine renewable resource. Seaweeds are rich in polysaccharides, minerals, proteins and vitamins. Documented antioxidant activity would elevate their value in the human diet as food and pharmaceutical supplements thus, in the present investigation the sea weed samples were collected in sterile condition and their antioxidant property for methanolic and petroleum ether extract was carried out by reducing power assay. The extraction process was carried out by drying and extracted the fine powder with different solvents. The extracted samples were then dissolved to a stock concentration of 1mg/ml and the reducing power assay was carried out with different concentrations of the sample.The methanolic extract of Cymodoceaeserrulata, Gracillariafolifera and Turbinariaconoides showed higher reducing power when compared to the standard.The petroleum ether extract of Cymodoceaerotundata and Ulvareticulata extracts showed higher activity when compared to the standard whereas Kappaphycussps showed the least activity when checked for its reducing power activity.

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References

Jadhav SJ, Nimbalkar SS, Kulkarni AD &Madhavi DL. (1995). “LIPID OXIDATION IN BIOLOGICAL AND FOOD SYSTEMS. IN: FOOD ANTIOXIDANTS.”Madhavi DL, Deshpande SS &Salunkhe DK (edition). New York.

Yamaguchi T, Takamura H, Matoba T &Terao J (1998). “HPLC METHOD FOR EVALUATION OF THE FREE RADICAL-SCAVENGING ACTIVITY OF FOODS BY USING 1, 1-DIPHENYL-2-PICRYLHYDRAZYL.” BiosciBiotechnolBiochem,62: 1201-1204.

Lim, S. N., Cheung, P. C. K., Ooi, V. E. C. and Ang, P. O. (2002)“EVALUATION OF ANTIOXIDATIVE ACTIVITY OF EXTRACTS FROM A BROWN SEAWEED, SARGASSUM SILIQUASTRUM.” Journal of Agricultural and Food Chemistry 50: 3862- 3866. DOI: https://doi.org/10.1021/jf020096b

Duan, X. J., Zhang, W. W, Li, X. M. and Wang, B. G. (2006)“EVALUATION OF ANTIOXIDANT PROPERTY OF EXTRACT AND FRACTIONS OBTAINED FROM A RED ALGA, POLYSIPHONIAURCEOLATA.” Food Chemistry 95: 37-43. DOI: https://doi.org/10.1016/j.foodchem.2004.12.015

Kuda, T., Kunii, T., Goto, H., Suzuki, T. and Yano, T. (2007)“VARIETIES OF ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES OF ECKLONIA STOLONIFERA AND ECKLONIA KUROME PRODUCTS HARVESTED AND PROCESSED IN THE NOTO PENINSULA, JAPAN. FOOD CHEMISTRY”. 103: 900-905. DOI: https://doi.org/10.1016/j.foodchem.2006.09.042

Kuda, T., Taniguchi, E., Nishizawa, M. and Araki, Y. (2002)“FATE OF WATER-SOLUBLE POLYSACCHARIDES IN DRIED CHORDA FILUMA BROWN ALGA DURING WATER WASHING”. Journal of Food Composition and Analysis 15: 3-9. DOI: https://doi.org/10.1006/jfca.2001.1037

Bequette& France (1997). “SEAWEED AT YOUR SERVICE”. UNESCO Courier50(11): 40-42.

Yan, X., Nagata, T., & Fan, X. (1998). “ANTIOXIDATIVE ACTIVITIES IN SOME COMMON SEAWEED”. Plant Foods for Human Nutrition, 52, 253–262. DOI: https://doi.org/10.1023/A:1008007014659

Jimenez-Escrig, A., Jimenez-Jimenez, I., Pulido, R., &Saura-Calixto, F. (2001). “ANTIOXIDANT ACTIVITY OF FRESH AND PROCESSED EDIBLE SEAWEEDS”. Journal of the Science of Food and Agriculture, 81, 530–534. DOI: https://doi.org/10.1002/jsfa.842

Ismail, A., & Hong, T. S. (2002). “ANTIOXIDANT ACTIVITY OF SELECTED COMMERCIAL SEAWEEDS”. Malaysian Journal of Nutrition, 8, 167–177.

Boukhari& Sophie (1998). “ANYONE FOR ALGAE?”UNESCO Courier51(7/8):31-32.

Jiménez-Escrig A & Sánchez-Muniz FJ (2000). “DIETARY FIBRE FROM EDIBLE SEAWEEDS: CHEMICAL STRUCTURE, PHYSICOCHEMICAL PROPERTIES AND EFFECTS ON CHOLESTEROL METABOLISM.” Nutrition Research20: 585-598.

Norziah MN &Ching YC (2000). “NUTRITIONAL COMPOSITION OF EDIBLE SEAWEEDS GRACILLARIA CHANGGI”. Food Chem68: 69-76.

Yuan, Y. V., Carrington, M. F. and Walsh, N. A. (2005)“EXTRACTS FROM DULSE (PALMARIA PALMATA) ARE EFFECTIVE ANTIOXIDANTS AND INHIBITORS OF CELL PROLIFERATION IN VITRO”. Food and Chemical Toxicology 43: 1073-1081. DOI: https://doi.org/10.1016/j.fct.2005.02.012

Bansemir, A., Blume, M., Schroder, S. and Lindequist, U. (2006)“SCREENING OF CULTIVATED SEAWEEDS FOR ANTIBACTERIAL ACTIVITY AGAINST FISH PATHOGENIC BACTERIA”. Aquaculture 252: 79-84. DOI: https://doi.org/10.1016/j.aquaculture.2005.11.051

Chew, Y. L., Lim, Y. Y., Omar, M. and Khoo, K. S. (2008)“ANTIOXIDANT ACTIVITY OF THREE EDIBLE SEAWEEDS FROM TWO AREAS IN SOUTH EAST ASIA”. LWT 41: 1067-1072. DOI: https://doi.org/10.1016/j.lwt.2007.06.013

Siriwardhana N, Lee KW, Kim SH, Ha JW, Jeon YJ (2003) “ANTIOXIDANT ACTIVITY OF HIZIKIAFUSIFORMIS ON REACTIVE OXYGEN SPECIES SCAVENGING AND LIPID PEROXIDATION INHIBITION”. Food SciTechnolInt 9:339–346.

Siriwardhana N, Lee KW, Kim SH, Ha JH, Park GT, Jeon YJ (2004) “LIPID PEROXIDATION INHIBITORY EFFECTS OF HIZIKIAFUSIFORMIS METHANOLIC EXTRACT ON FISH OIL AND LINOLEIC ACID”. Food SciTechnolInt 10:65–72.

Cornish ML, Garbary DJ (2010) “ANTIOXIDANTS FROM MACROALGAE: POTENTIAL APPLICATIONS IN HUMAN HEALTH AND NUTRITION”. Algae 25:000–000.

Le Tutour B, Benslimane F, Gouleau MP, Gouygou JP, Saadan B, Quemeneur (1998) “ANTIOXIDANT AND PRO-OXIDANT ACTIVITIES OF BROWN ALGAE, LAMINARIADIGITATA, HIMANTHALIA ELONGATE, FUCUSVESICULOSUS, FUCUSSERRATUS AND ASCOPHYLLUMNODOSUM”. J ApplPhycol 10:121–129.

Zhang Q, Yu P, Li Z, Zhang H, Xu Z, Li P (2003) “ANTIOXIDANT ACTIVITIES OF SULFATED POLYSACCHARIDE FRACTIONS FROM PORPHYRAHAITANESIS”. J ApplPhycol 15:305–310.

Yuan H, Zhang W, Li X, Lü X, Li N, Gao X, Song J (2005) “PREPARATION AND IN VITRO ANTIOXIDANT ACTIVITY OF Κ-CARRAGEENAN OLIGOSACCHARIDES AND THEIR OVERSULFATED, ACETYLATED, AND PHOSPHORYLATED DERIVATES”. Carbohydr Res 340:685– 690. DOI: https://doi.org/10.1016/j.carres.2004.12.026

Qi H, Zhao T, Zhang Q, Li Z, Xing R (2005) “ANTIOXIDANT ACTIVITY OF DIFFERENT MOLECULAR WEIGHT SULFATED POLYSACCHARIDES FROM ULVAPERTUSAKJELLM (CHLOROPHYTA)”. J ApplPhycol 17:527–534.

Yan X, Li X, Zhou C, FanX(1996) “PREVENTION OF FISH OIL RANCIDITY BY PHLOROTANNINS FROM SARGASSUM KELLMANIANUM”. J. Appl. Phycol. 8: 201–203. DOI: https://doi.org/10.1007/BF02184972

Yan X, Li X, Fan X, Zhou C (1997) “STUDIES ON EXTRACTION PROCEDURE AND ANTIOXIDATIVE ACTIVITY OF PHLOROTANNINS FROM SARGASSUM KJELLMANIANUM”. Chinese J. Oceanol. Limnol. 15: 42–45. DOI: https://doi.org/10.1007/BF02850581

Wei Y, Xu Z (2003) “STUDIES ON ANTIOXIDATIVE ACTIVITY OF HIGH MOLECULAR WEIGHT POLYPHENOLS FROM TWO KINDS OF BROWN ALGAE”. Zhongcaoyao 34: 3170–319.

Nakamura T, Nagayama K, Uchida K, Tanaka R (1996) “ANTIOXIDANT ACTIVITY OF PHLOROTANNINS ISOLATED FROM THE BROWN ALGA EISENIABICYCLIS”. Fish. Sci. 62: 923–926. DOI: https://doi.org/10.2331/fishsci.62.923

Chkhikvishvili ID, Ranazanov ZM (2000) “PHENOLIC SUBSTANCES OF BROWN ALGAE AND THEIR ANTIOXIDANT ACTIVITY. PRIKLADNAYA BIOKHIMIYA I MIKROBIOLOGIYA” 36: 336–338.

Kang HS, Chung HY, Jung JH, Son BW, Choi JS (2003a) “A NEW PHLOROTANNIN FROM THE BROWN ALGA ECKLONIA STOLONIFERA”. Chem. Pharmaceut. Bull. 51: 1012–1014. DOI: https://doi.org/10.1248/cpb.51.1012

Kang HS, Chung HY, Kim JY, Son BW, Jung HA, Choi JS (2004) “INHIBITORY PHLOROTANNINS FROM THE EDIBLE BROWN ALGA ECKLONIA STOLONIFERAON TOTAL REACTIVE OXYGEN SPECIES (ROS) GENERATION”. Arch. Pharmacol. Res. 27: 194–198. DOI: https://doi.org/10.1007/BF02980106

Rupérez P, Ahrazem O, Leal JA (2002)“POTENTIAL ANTIOXIDANT CAPACITY OF SULFATED POLYSACCHARIDES FROM THE EDIBLE MARINE BROWN SEAWEED FUCUS VESICULOSUS”. J Agric Food Chem. 2002 Feb 13; 50(4):840-5.