MOLECULAR MARKER ASSISTED STUDIES ON GENETIC DIVERSITY IN BRASSICA SPECIES
DOI:
https://doi.org/10.29121/granthaalayah.v3.i7.2015.2986Keywords:
RAPD, Polymorphism, Brassica CampestrisAbstract [English]
Genetic diversity of seven genotypes of Brassica campestris was evaluated by using RAPD primers. A total of 15 DNA bands were revealed wherein 11 polymorphic bands were observed. Highest polymorphism was showed by the primer OPA 7 (87.50%) and lowest by the primer OPA 9 (57.14%). Relatively higher genetic diversity was found among the collected individuals. A maximum similarity based on Jaccard coefficient was showed highest and lowest value (0.750) and (0.364) respectively. The UPGMA cluster analysis revealed three main group of clusters in which one group was found to be sub grouped and other two main groups were found to be divergent. Cluster analysis revealed distant relationship among all the cultivars used in this study.Based on our study, RAPD analysis proved to be a useful tool in assessing the genetic diversity of leafy Brassica germplasm. These results also proved that RAPD markers are highly suitable and efficient tool for genetic characterization of many plant species including oilseed rape and other species.
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References
PrakashS, Hinta K. Taxonomy, cytogenetic and origin of crop Brassica,Opera Bot. 1980, 55: 1-57.
Toxeopus H, Oost EH, Reuling G. Current aspects of the taxonomy of cultivated Brassica species and use of B. rapa L. versus B. campestris L. and a proposal for a new intraspecific classification of B. rapa L. Crucifer Newsletter. 1984, 9: 55-57.
Winter P, Kohl G. Molecular marker technologies for plant improvement. World J MicrobBiot.1995, 11: 438-448. DOI: https://doi.org/10.1007/BF00364619
Mullis KB, Faloona FA, Scharf SJ, Saiki SK, Horn GT, Erlich HA. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol. 1986; 51: 263-273. DOI: https://doi.org/10.1101/SQB.1986.051.01.032
Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990; 18 (24): 7213-7218. DOI: https://doi.org/10.1093/nar/18.24.7213
Ayad WG, Hodking A, Jaradat A and Rao VR.Molecular genetic techniques for plant genetic resources.IPGRI Workshop: Rome, Italy. 1995, 9-11.
Agarwal M, ShrivastavaN,Padh H. Advances in molecular marker techniques and their application in plant sciences. Plant Cell Rep. 2008, 27: 617-631. DOI: https://doi.org/10.1007/s00299-008-0507-z
Pervaiz, ZH, Rabbani MA, Shinwari ZK, Masood MS,Malik SA. Assessment of genetic variability in rice (Oryza sativa L.) germplasm from Pakistan using RAPD markers. Pak. J. Bot. 2010, 42: 3369-3376.
Paterson AH, Damon S,Hewitt JD, Zamir D, Rabino VHD, Lincoln SE, Lander SS, Tanksley SD. Mendelian factors underlying quantitative traits in tomato: Comparison across species, generations and environments. Genetics.1991, 127: 181-197. DOI: https://doi.org/10.1093/genetics/127.1.181
Rabbani MA, Masood MS, Shinwari ZK, Yamaguchi-Shinozaki K. Genetic analysis of basmati and non-basmati Pakistani rice (Oryza sativa L.) cultivars using microsatellite markers. Pak. J. Bot. 2010, 42: 2551-2564.
Ren JR, Mcferson LR, Kresovich S, Lamboy WF. Identities and relationship among Chinese vegetable Brassicas as determined by random amplified polymorphic DNA markers. J. Am. Soc. Hort. Sci. 1995, 120: 548-555. DOI: https://doi.org/10.21273/JASHS.120.3.548
An X, Chen BY, Fu TD, Liu LH. Genetic diversity analysis among Chinese landraces in Brassica juncea using RAPD markers.10th International Rapeseed Congress, Canberra, Australia, 2000
Doyle JJ and Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990, 12: 13-15.
RohlfFJ.Numerical taxonomy and multivariate analysis system. New York: Exeter Software Setauket, NTSYS pc 2.02, 1998.
Hu J, Li G, Struss D, Quiros CF. SCAR and RAPD markers associated with 18-carbon fattyacidsin rape seed, Brassicanapus. Plant Breed.1999, 118: 145-150. DOI: https://doi.org/10.1046/j.1439-0523.1999.118002145.x
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