EFFECT OF CARBENECILLIN AND CEFOTAXIME ON CALLUS INDUCTION AND DEVELOPMENT SOMATIC EMBRYOS OF MALAYSIAN INDICA RICE MR219

Authors

  • Zuraida Ab Rahman Biotechnology & Nanotechnologyresearch Centre, Malaysian Agricultural Research And Development Institute, Mardi Hq, Persiaran Mardi-Upm, 43400 Serdang Selangor, Malaysia
  • Ayu Nazreena Othman Biotechnology & Nanotechnologyresearch Centre, Malaysian Agricultural Research And Development Institute, Mardi Hq, Persiaran Mardi-Upm, 43400 Serdang Selangor, Malaysia
  • Amirah Balqis Amir Amran School of Agriculture Science & Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200, Besut, Terengganu, Malaysia
  • Nur Najwa Arifah Basiron Biotechnology & Nanotechnologyresearch Centre, Malaysian Agricultural Research And Development Institute, Mardi Hq, Persiaran Mardi-Upm, 43400 Serdang Selangor, Malaysia
  • Norliza Abu Bakar Biotechnology & Nanotechnologyresearch Centre, Malaysian Agricultural Research And Development Institute, Mardi Hq, Persiaran Mardi-Upm, 43400 Serdang Selangor, Malaysia
  • Zulkifli Ahmad Seman Biotechnology & Nanotechnologyresearch Centre, Malaysian Agricultural Research And Development Institute, Mardi Hq, Persiaran Mardi-Upm, 43400 Serdang Selangor, Malaysia

DOI:

https://doi.org/10.29121/granthaalayah.v10.i2.2022.4509

Keywords:

Callus Induction, Carbenicillin and Cefotaxime, MR219

Abstract [English]

Carbenicillin and cefotaxime, two antibiotics routinely employed to exclude Agrobacterium tumefaciens during plant transformation, were studied for their effects on callus growth and the formation of somatic embryos. Antibiotics were tested to see how they responded to the regeneration of Malaysian indica rice cv. MR219. In a preliminary study, callus fresh weight gain appeared to be improved in media containing 200 mg/L carbenicillin and 300-400 mg/L cefotaxime but was severely hindered in media containing higher concentrations. Somatic embryos were discovered in greater numbers in the medium containing 200 mgl-1 carbenicillin or 300 mgl-1 cefotaxime, at 76 and 71, respectively. It was possible that these antibiotics showed a positive response on somatic embryos development when compared to those without antibiotics. Carbenicillin and cefotaxime concentrations greater than 300 mg/L should not be utilized for the process

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References

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Published

2022-03-05

How to Cite

Rahman, Z. A., Othman, A. N., Amir Amran, A. B., Arifah Basiron, N. N., Abu Bakar, N., & Seman, Z. A. (2022). EFFECT OF CARBENECILLIN AND CEFOTAXIME ON CALLUS INDUCTION AND DEVELOPMENT SOMATIC EMBRYOS OF MALAYSIAN INDICA RICE MR219. International Journal of Research -GRANTHAALAYAH, 10(2), 71–75. https://doi.org/10.29121/granthaalayah.v10.i2.2022.4509

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