EFFICACY OF CLERODENDRUM INFORTUNATUM AND CHROMOLAENA ODORATA ON CARBOHYDRATE CONTENT IN HAEMOLYMPH OF THE SIXTH INSTAR LARVAE OF ORTHAGA EXVINACEA HAMPSON

Jagadeesh Nambiar; K.R. Ranjini

doi:10.29121/ijetmr.v5.i4.2018.209

Authors

Jagadeesh G. Nambiar Department of Zoology, Malabar Christian College, Calicut, India
K. R. Ranjini Associate Professor, Department of Zoology, Malabar Christian College, Calicut, India

DOI:

https://doi.org/10.29121/ijetmr.v5.i4.2018.209

Keywords:

Orthaga Exvinacea, Clerodendrum Infortunatum, Chromolaena Odorata, Haemolymph, Carbohydrate

Abstract

Orthaga exvinacea is one of the major pests of mango crop and the caterpillars defoliate the leaves and thereby reduce the crop yield. Use of synthetic insecticide is the quick method for the control this pest but its uncontrolled usage has resulted in serious lethal effects on non-target organisms and environmental pollution. Botanical insecticides are very effective, safe and ecologically acceptable. In the present study, the impact of methanolic leaf extracts of Clerodendrum infortunatum and Chromolaena odorata on carbohydrate concentration in the haemolymph of sixth instar larvae of O. exvinacea was studied under laboratory conditions. The different concentrations (1% to 5%) of each botanical treated mango leaves were fed to the sixth instar. After 48 hours, larvae were sacrificed to collect haemolymph and the quantitative estimation of carbohydrate has been done. The results showed that there was some noticeable decrease in the amount of carbohydrate in the treated larvae when compared to control. The decrease in level of carbohydrate concentration was correlated with the increase in concentration of botanicals. Among the botanicals tested C. odorata possessed more efficacy than that of C. infortunatum and this experiment reveals the potency of both botanicals to be used as natural biopesticides against this pest.

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References

Singh, R., Lakhanpal, S. C. and Karkara, B. K. (2006). Incidence of mango leaf webber, Orthaga euadrusalis (Hampson) in high density plantation of mango at Dhaulakhan in Himachal Pradesh. Insect. Environment., 11:178-179.

Cherian, M. C. and Ananthanarayanan, K. P. (1943). The mango shoot webber Orthaga exvinacea Hampson and its control. Madras. Agric. J., 31(11): 321-323.

Mordue, A. J. and Blackwell, A. (1993). Azadirachtin: an update. J. Insect Physiol., 39(11): 903- 924.

Breuer, M. and Schmidt, G. H. (1995). Influence of a short period treatment with Melia azedarach extract on food intake and growth of the larvae of Spodoptera frugiperda (J.E. Smith) (Lep. Noctuidae). J. Plant Diseases Protect. 102: 633-654.

Hiremath, I. G., Ahn, Y. J. and Kim, S. I. (1997). Insecticidal activity of Indian plant extracts against Nilaparvata lugens (Lepidoptera: Yponomeutidae). Appl. Entomol. Zool. 32: 533-538.

Zhao, B., Grant, G. G., Langevin, D. and MacDonald, L. (1998). Deterring and inhibiting effects of quinolizidine alkaloids on spruce budworm (Lepidoptera: Tortricidae) oviposition. Environ. Entomol. 27: 984-992.

Muthukrishnan, J. and Pushpalatha, E. (2001). Effects of plant extracts on fecundity and fertility of mosquitoes. J. Appl. Ent., 125: 31-35.

Sadek, M. M. (2003). Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis (Lep., Noctuidae). J. Appl. Ent., 127: 396- 404.

Roy Choudhary, N. (1994). Antifeedant and insecticidal activities of Clerodendrum species against rice weevil. J. Appl. Zool. Res., 5(1): 13- 16.

Pandey, R., Verma, R. K. and Gupta, M. (2005). Neo clerodane ditepenoids from Clerodendrum inerme. Phytochemistry., 66: 643- 648.

Chandrika, M. and Nair, C. P. R. (2000). Effect of Clerodendrum infortunatum on grubs of coconut Rhinoceros beetle, Oryctes rhinoceros L. In: Recent Advances in Plantation Crops Research (Muralidharan, N. and Rajkumar, R. Eds.). pp. 297- 299.

Sreelatha, K. B. and Geetha, P. R. (2010). Distruption of oocyte development and vitellogenesis in Oryctes rhinoceros treated with methanoloic extract of Eupatorium odoratum leaves. J. Biopestics., 3(1): 253-258.

Roy, S., Mukhopadhyay, A. and Gurusubramanian, G. (2009). Antifeedant and insecticidal activity of Clerodendron infortunatum Gaertn. (Verbinaceae) extract on tea mosquito bug, Helopeltis theivora Waterhouse (Hetoraptera: Miridae). Res. Crops., 10(1): 152-158.

Sreelatha, K. B. and Geetha, P. R. (2011). Pesticidal effects of Clerodendrum infortunatum on the fat body of Oryctes rhinoceros (Linn.) male. J. Biopestics., 4(1): 13-17.

Rajamma, P. (1982). Effect of some organic materials on the control of sweet potato weevil Cylas formicarius Fab. J. Root Crops., 8(1-2): 64-65.

Roeder, K. P. (1953). Reflex activity and ganglion function. In: Insect Physiology, John Wiley and Sons, Publishers, New York and London, pp 463-487.

Ranjini, K. R. and Mohamed, U. V. K. (2004). Changes in the concentration of total carbohydrates in the fat body of Orthaga exvinacea Hampson (Pyralidae: Lepidoptera) during development. J. Adv. Zool., 25(1 and 2): 51-53.

Plummer, D. T. (1971). Carbohydrates. In an introduction to Practical Biochemistry. Tata Mc Graw Hill Book Company Ltd. Bombay and New Delhi. 5: pp.104.

Medhini, N., Divakar, Y. G. and Manjulakumari, D. (2012). Effect of Calendula officinalis extracts on the nutrient components of different tissues of tobacco cutworm, Spodoptera litura Fabricius. J. Biopestics., 5(Supplementary): 139-144.

Shoukry, I. F., Khalaf, A. A., Hussein, K. T. and Khater, K. S. (2003). Toxicological evaluation of some botanical oils on biochemical aspects in the Indian meal moth Plodia interpuncetella (HB) (Lepidoptera: Pyralidae). Egypt. J. Biol., 5: 155- 163.

Vijayaraghavan, C., Sivakumar, C., Zadda kavitha and Sivasubramanian, P. (2010). Effect of plant extracts on biochemical components of cabbage leaf webber, Crocidolomia binotalis Zeller. J. Biopestics. 3(Special Issue): 275-277.

Shoukry, I. F., Khalaf, A. A., Hussein, K. T. and Khater, K. S. (2002 a). Toxicological and histopathological effects of three fixed oils extracts on the Indian meal moth, Plodia interpunctella HB. (Lepidoptera: Pyralidae). Ain. Shams. Sci. Bull., 40: 137-154.

Shoukry, I. F., Khalaf, A. A., Hussein, K. T. and Khater, K. S. (2003). Toxicological evaluation of some botanical oils on biochemical aspects in the Indian meal moth Plodia interpuncetella (HB) (Lepidoptera: Pyralidae). Egypt. J. Biol., 5: 155- 163.

Abo El ghar, G. E., Khalil, M. E. and Eid, T. M. (1995). Some biochemical effects of plant extracts in the black cutworm, Agrotis ipsilon (Hufnagel). Bull. Ent. Soc. Egypt, Econ. Ser., 22: 85-97.

Koul, O. (1999). Insect growth regulating and antifeedant effects of neem extracts and azadirachtin on two aphid species of ornamental plants. J. Bioscien., 24: 85-90.

Abdul Razak, T. and Sivasubramanian, P. (2007). Effects of three botanical oils on carbohydrate content in Cheilomenes sexmaculata Fabricus and Chrysoperla carnea Stephens. Asian. J. Biochem., 2(2): 124-129.

Sak, O., Uckan, F. and Eegin, E. (2006). Effects of cypermethrin on total body weight, glycogen, protein, and lipid contents of Pimpla turionellaei (L.) (Hymenoptera: Ichneumonidae). Belgium. J. Zool., 136(1): 53-58.

Sharma, P., Mohan, L., Dua, K. K. and Srivastava, C. N. (2011). Status of carbohydrate, protein and lipid profile in the mosquito larvae treated with certain phytoextracts. Asian. Pac. J. Trop. Dis., 4(4): 301-304.

Seyoum, E., Bateman, R. P. and Charnley, A. K. (2002). The effect of Metarhizium anisopliae var acridum on haemolymph energy reserves and flight capability in the desert locust, Schistocerca gregaria. J. Appl. Entomol., 126: 119-124.

Etebari, K., Bizhannia, A. R., Sorati, R. and Matindoost, L. (2006). Biochecimal changes in haemolymph of silkworm larvae due to pyriproxyphen residue. Pestic. Biochem. Physiol., 88: 14- 19.