MOLECULAR IDENTIFICATION OF FUNGI ISOLATED FROM POSTHARVEST MANGO FRUITS AND EVALUATION OF ESSENTIAL OILS ACTIVITY OF GROWTH INHIBITION
DOI:
https://doi.org/10.29121/granthaalayah.v9.i3.2021.3782Keywords:
Mangifera indica; fungi fruits; sequencing; essential oils; growth inhibition.Abstract [English]
This work aimed to perform the molecular identification of fungi mango fruits, in addition to verifying the effect of the essential oils for microbial control. Pericarp segments containing injured and healthy areas of mango fruits were initially disinfested, and then incubated in PDA culture medium at 25 °C. From all isolated material, UDC03, UDC04, UDC09 and UDC12 were preselected, which presented homogeneous cultures. The UDC04 and UDC12 isolates were submitted to the sequencing of the ITS region for presenting mycelial growth both greater and morphologically distinct of fungi from Colletotrichum genus. When BLAST is used in species identification, it was observed that UDC04 and UDC12 belong to Botryosphaeria dothidea and Neofusicoccum parvum, respectively. Regarding the control of UDC04, it was observed that BDA culture medium containing 1.5 to 2% of lemongrass and eucalyptus oils resulted in complete inhibition. For UDC12, mycelial growth inhibition was detected with the use of 1% and 1.5% essential oils of eucalyptus and lemongrass, respectively. The chia essential oil was not effective for the isolates control. Finally, the results reveal the importance of molecular identification of these phytopathogens associated with the establishment of an alternative control of fruits due to problems of contamination with chemical products.
Downloads
Download data is not yet available.
References
[1] Alfenas, A. C., et al, Isolamento de fungos fitopatogênicos. Métodos em fitopatologia. Viçosa: UFV; 2016, 55-92.
[2] Andrade, E. H. A., Zoghbi, M. das G. B., and Lima, M. da P. Chemical composition of the essential oils of Cymbopogon citratus (DC.) Stapf cultivated in North of Brazil. Journal of essential oil bearing plants, Vol. 12, No. 1, Mar. 2009, 41-45.
[3] Bakkali, F., et al. Biological effects of essential oils–a review. Food and chemical toxicology, Vol. 46, No. 2, Feb. 2008, 446-475.
[4] Batista, D. D. C., et al. Doenças da mangueira. Informe Agropecuário, Vol. 37, No. 290, Nov. 2016, 82-91.
[5] Berton, O., et al., Doenças fúngicas dos ramos: podridão-branca – Botryosphaeria dothidea In: EPAGRI. A cultura da macieira. Florianópolis: EPAGRI, 2006, 567-571.
[6] Boneti, J. I. S. Doenças da macieira (Malus domestica Bork). In: Boneti JIS, Ribeiro LG, Katsurayama Y (Eds.) Manual de identificação de doenças e pragas da macieira. Florianópolis: EPAGRI, 1999, 13-95.
[7] Brum, R. B. C. S., et al. Atividade antifúngica de óleos essenciais sobre fungos fitopatogênicos. Magistra, Vol. 26, No. 3, Jul/Set. 2014, 361-371.
[8] Carnelossi, P. R., et al. Óleos essenciais no controle pós-colheita de Colletotrichum gloeosporioides em mamão. Revista Brasileira de Plantas Medicinais, Vol. 11, No. 4, Abr. 2009, 399-406.
[9] Costa, A. V., et al. Chemical composition of essential oil from Eucalyptus citriodora leaves and insecticidal activity against Myzus persicae and Frankliniella schultzei. Journal of Essential Oil Bearing Plants, Vol. 18, No. 2, Jun. 2015, 374-381.
[10] Dias, J. A. Botryosphaeria dothidea. Defesa vegetal.net, 2014. Retrieved from: http://www.defesavegetal.net/botsdo.Acesso 10 agot. 2018.
[11] Oliveira, F. da S. de, et al. Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis. Research, Society and Development, Vol. 9, No. 7, June 2020, 691974697.
[12] Oliveira Costa, V. S. de, et al. Species of Botryosphaeriaceae associated on mango in Brazil. European Journal of Plant Pathology, Vol. 127, No. 4, May 2010, 509-519.
[13] El-Mohamedy, R. S. R. Plant essential Oils for controlling Plant pathogenic Fungi. In: Volatiles and Food Security. Singapore: Springer, 2017, 171-198.
[14] Elshafie, H. S. et al. Chemical composition and antimicrobial activity of chia (Salvia hispanica L.) essential oil. European Food Research and Technology, Vol. 244, No. 9, Sept. 2018, 1675-1682.
[15] Faiza, A., Aouich, A., and Redha, B. The Chemical Composition and the Antifungal Activity of the Essential Oil of Origanum glandulosum against Neofusicoccum parvum. Jordan Journal of Biological Sciences, Vol. 11, No. 2, June 2018, 117-121.
[16] Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95 ⁄ 98 ⁄ NT. Nucleic Acids Symposium Series, Vol 41, 1999, 95–98.
[17] Henz, G. P. Postharvest losses of perishables in Brazil: what do we know so far? Horticultura Brasileira, Vol. 35, No. 1, Jan/ Mar. 2017, 6-13.
[18] IBM Corp. Released. IBM SPSS Statistics for Windows. Version 27.0 [Programa de computador]. Armonk, Nova York: IBM Corp, 2020.
[19] Junior, I. T. S., Sales, N. L., and Martins, E. R. Efeito fungitóxico de óleos essenciais sobre Colletotrichum gloeosporioides, isolado do maracujazeiro amarelo. Biotemas, Vol. 22, No. 3, Mar. 2009, 77-83.
[20] Kamle, M., and Kumar, P. Colletotrichum gloeosporioides: Pathogen of Anthracnose Disease in Mango (Mangifera indica L.). In: Kumar, P., et al. (eds) Current Trends in Plant Disease Diagnostics and Management Practices. Springer, Cham, 2016, 207-219.
[21] Kuramae-Izioka, E. E. A rapid, easy and high yield protocol for total genomic DNA isolation of Colletotrichum gloesporioides and Fusarium oxysporum. Revista Unimar, Marília, Vol. 19, No. 3, Aug. 1997, 683-689.
[22] Lee, Y. S., et al. Antifungal activity of Myrtaceae essential oils and their components against three phytopathogenic fungi. Flavour and Fragrance Journal, Vol. 23, No. 1, Jan. 2008, 23-28.
[23] Lima, J. R. F. de, et al. Análise do mercado de manga produzida no Vale do São Francisco: cenário atual e perspectivas para o curto prazo. In Embrapa Semiárido-Artigo em anais de congresso (ed) Congresso Da Sociedade Brasileira de Economia, Administração e Sociologia Rural Do Regional Nordeste, Juazeiro: UNIVASF: SOBER-NE, Nov. 2018, 1-19.
[24] Majewska, E., et al. Lemongrass (Cymbopogon citratus) essential oil: extraction, composition, bioactivity and uses for food preservation-a review. Polish Journal of Food and Nutrition Sciences, Vol. 69, No. 4, Nov. 2019, 327-341.
[25] Martinazzo, A. P., Oliveira, F. da S. de, and Teodoro, C. E. de S. Antifungal activity of Cymbopogon citratus essential oil against Aspergillus flavus. Ciência e Natura, Vol. 41, No. 20 July 2019, 1-8.
[26] Mirhendi, H., et al. A one enzyme PCR RFLP assay for identification of six medically important Candida species. Nihon Ishinkin Gakkai Zasshi, Vol. 47, No.3, Feb. 2006, 225-229.
[27] Pereira, F. D. O., et al. Antifungal activity of geraniol and citronellol, two monoterpenes alcohols, against Trichophyton rubrum involves inhibition of ergosterol biosynthesis. Pharmaceutical biology, Vol. 53, No. 2, Feb. 2015, 228-234.
[28] Ploetz, R. C., and Freeman, S. Foliar, floral and soilborne diseases. In: Litz, R. E. (ed.) The mango: botany, production and uses. 2nd ed. Wallingford: CABI, 2009, 231-302.
[29] Puškárová, A., et al. The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells. Scientific reports, Vol, 7, No. 1, Aug. 2017, 1-11.
[30] Rios, J. L.. Essential oils: What they are and how the terms are used and defined. In: Preedy, V. R. (ed) Essential oils in food preservation, flavor and Safety. Academic Press, 2016, 3-10.
[31] Santos, G. R. D., et al., Effect of essential oils of medicinal plants on leaf blotch in Tanzania grass. Revista Ciência Agronômica, Vol. 44, No. 3, July/ Sept. 2013, 587-593.
[32] Santos, S. F. dos, et al. Post-harvest losses of fruits and vegetables in supply centers in Salvador, Brazil: Analysis of determinants, volumes and reduction strategies. Waste Management, Vol. 101, No. s/n, Jan. 2020, 161-170.
[33] Saaiman, W. C. Biology and control of Nattrassia mangiferae. In: Lavi, U. et al. (eds.). Proceedings of the 5th International Mango Symposium. Israel: Leuven International Society for Horticultural Science, 1997, 558-565.
[34] Sarkhosh, A., et al. In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya. Plant Protection Science, Vol. 54, No. 3, May. 2018, 153-162.
[35] Statsoft, Inc. STATISTICA (data analysis software system), version 7, 2004.
[36] Tavares, S. C. C. de H. Epidemiologia e manejo integrado de Botryodiplodia theobromae - Situação atual no Brasil e no mundo. Fitopatologia Brasileira, Vol. 27, No. s/n, 2002, 46–52.
[37] Terao, D., Nechet, K. de L., and Halfeld-Vieira, B. de A. Competitive and colony layer formation ability as key mechanisms by yeasts for the control Botryosphaeria dothidea fruit rot of mango. Tropical Plant Pathology, Vol. 42, No. 6, Dec. 2017, 451-457
[38] Terao, D., Batista, D. C., e Barbosa, M. A. G. Doenças em pós-colheita de manga. Circular técnica, Vol. s/v, No. 105, Dez. 2013, 1-8.
[39] Vyshali, P., Saraswathi, K.J T., and Mallavarapu, G. R. Chemical composition of the essential oils of Cymbopogon citratus (DC.) stapf grown in three locations in South India. Journal of Essential Oil Bearing Plants, Vol. 18, No. 1, Apr. 2015, 230-235.
[40] Zhang, Z., et al. Antifungal activity of monoterpenes against Botryosphaeria dothidea. Natural Product Communications, Vol. 13, No. 12, Nov. 2018, 1721-1724.
[2] Andrade, E. H. A., Zoghbi, M. das G. B., and Lima, M. da P. Chemical composition of the essential oils of Cymbopogon citratus (DC.) Stapf cultivated in North of Brazil. Journal of essential oil bearing plants, Vol. 12, No. 1, Mar. 2009, 41-45.
[3] Bakkali, F., et al. Biological effects of essential oils–a review. Food and chemical toxicology, Vol. 46, No. 2, Feb. 2008, 446-475.
[4] Batista, D. D. C., et al. Doenças da mangueira. Informe Agropecuário, Vol. 37, No. 290, Nov. 2016, 82-91.
[5] Berton, O., et al., Doenças fúngicas dos ramos: podridão-branca – Botryosphaeria dothidea In: EPAGRI. A cultura da macieira. Florianópolis: EPAGRI, 2006, 567-571.
[6] Boneti, J. I. S. Doenças da macieira (Malus domestica Bork). In: Boneti JIS, Ribeiro LG, Katsurayama Y (Eds.) Manual de identificação de doenças e pragas da macieira. Florianópolis: EPAGRI, 1999, 13-95.
[7] Brum, R. B. C. S., et al. Atividade antifúngica de óleos essenciais sobre fungos fitopatogênicos. Magistra, Vol. 26, No. 3, Jul/Set. 2014, 361-371.
[8] Carnelossi, P. R., et al. Óleos essenciais no controle pós-colheita de Colletotrichum gloeosporioides em mamão. Revista Brasileira de Plantas Medicinais, Vol. 11, No. 4, Abr. 2009, 399-406.
[9] Costa, A. V., et al. Chemical composition of essential oil from Eucalyptus citriodora leaves and insecticidal activity against Myzus persicae and Frankliniella schultzei. Journal of Essential Oil Bearing Plants, Vol. 18, No. 2, Jun. 2015, 374-381.
[10] Dias, J. A. Botryosphaeria dothidea. Defesa vegetal.net, 2014. Retrieved from: http://www.defesavegetal.net/botsdo.Acesso 10 agot. 2018.
[11] Oliveira, F. da S. de, et al. Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis. Research, Society and Development, Vol. 9, No. 7, June 2020, 691974697.
[12] Oliveira Costa, V. S. de, et al. Species of Botryosphaeriaceae associated on mango in Brazil. European Journal of Plant Pathology, Vol. 127, No. 4, May 2010, 509-519.
[13] El-Mohamedy, R. S. R. Plant essential Oils for controlling Plant pathogenic Fungi. In: Volatiles and Food Security. Singapore: Springer, 2017, 171-198.
[14] Elshafie, H. S. et al. Chemical composition and antimicrobial activity of chia (Salvia hispanica L.) essential oil. European Food Research and Technology, Vol. 244, No. 9, Sept. 2018, 1675-1682.
[15] Faiza, A., Aouich, A., and Redha, B. The Chemical Composition and the Antifungal Activity of the Essential Oil of Origanum glandulosum against Neofusicoccum parvum. Jordan Journal of Biological Sciences, Vol. 11, No. 2, June 2018, 117-121.
[16] Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95 ⁄ 98 ⁄ NT. Nucleic Acids Symposium Series, Vol 41, 1999, 95–98.
[17] Henz, G. P. Postharvest losses of perishables in Brazil: what do we know so far? Horticultura Brasileira, Vol. 35, No. 1, Jan/ Mar. 2017, 6-13.
[18] IBM Corp. Released. IBM SPSS Statistics for Windows. Version 27.0 [Programa de computador]. Armonk, Nova York: IBM Corp, 2020.
[19] Junior, I. T. S., Sales, N. L., and Martins, E. R. Efeito fungitóxico de óleos essenciais sobre Colletotrichum gloeosporioides, isolado do maracujazeiro amarelo. Biotemas, Vol. 22, No. 3, Mar. 2009, 77-83.
[20] Kamle, M., and Kumar, P. Colletotrichum gloeosporioides: Pathogen of Anthracnose Disease in Mango (Mangifera indica L.). In: Kumar, P., et al. (eds) Current Trends in Plant Disease Diagnostics and Management Practices. Springer, Cham, 2016, 207-219.
[21] Kuramae-Izioka, E. E. A rapid, easy and high yield protocol for total genomic DNA isolation of Colletotrichum gloesporioides and Fusarium oxysporum. Revista Unimar, Marília, Vol. 19, No. 3, Aug. 1997, 683-689.
[22] Lee, Y. S., et al. Antifungal activity of Myrtaceae essential oils and their components against three phytopathogenic fungi. Flavour and Fragrance Journal, Vol. 23, No. 1, Jan. 2008, 23-28.
[23] Lima, J. R. F. de, et al. Análise do mercado de manga produzida no Vale do São Francisco: cenário atual e perspectivas para o curto prazo. In Embrapa Semiárido-Artigo em anais de congresso (ed) Congresso Da Sociedade Brasileira de Economia, Administração e Sociologia Rural Do Regional Nordeste, Juazeiro: UNIVASF: SOBER-NE, Nov. 2018, 1-19.
[24] Majewska, E., et al. Lemongrass (Cymbopogon citratus) essential oil: extraction, composition, bioactivity and uses for food preservation-a review. Polish Journal of Food and Nutrition Sciences, Vol. 69, No. 4, Nov. 2019, 327-341.
[25] Martinazzo, A. P., Oliveira, F. da S. de, and Teodoro, C. E. de S. Antifungal activity of Cymbopogon citratus essential oil against Aspergillus flavus. Ciência e Natura, Vol. 41, No. 20 July 2019, 1-8.
[26] Mirhendi, H., et al. A one enzyme PCR RFLP assay for identification of six medically important Candida species. Nihon Ishinkin Gakkai Zasshi, Vol. 47, No.3, Feb. 2006, 225-229.
[27] Pereira, F. D. O., et al. Antifungal activity of geraniol and citronellol, two monoterpenes alcohols, against Trichophyton rubrum involves inhibition of ergosterol biosynthesis. Pharmaceutical biology, Vol. 53, No. 2, Feb. 2015, 228-234.
[28] Ploetz, R. C., and Freeman, S. Foliar, floral and soilborne diseases. In: Litz, R. E. (ed.) The mango: botany, production and uses. 2nd ed. Wallingford: CABI, 2009, 231-302.
[29] Puškárová, A., et al. The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells. Scientific reports, Vol, 7, No. 1, Aug. 2017, 1-11.
[30] Rios, J. L.. Essential oils: What they are and how the terms are used and defined. In: Preedy, V. R. (ed) Essential oils in food preservation, flavor and Safety. Academic Press, 2016, 3-10.
[31] Santos, G. R. D., et al., Effect of essential oils of medicinal plants on leaf blotch in Tanzania grass. Revista Ciência Agronômica, Vol. 44, No. 3, July/ Sept. 2013, 587-593.
[32] Santos, S. F. dos, et al. Post-harvest losses of fruits and vegetables in supply centers in Salvador, Brazil: Analysis of determinants, volumes and reduction strategies. Waste Management, Vol. 101, No. s/n, Jan. 2020, 161-170.
[33] Saaiman, W. C. Biology and control of Nattrassia mangiferae. In: Lavi, U. et al. (eds.). Proceedings of the 5th International Mango Symposium. Israel: Leuven International Society for Horticultural Science, 1997, 558-565.
[34] Sarkhosh, A., et al. In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya. Plant Protection Science, Vol. 54, No. 3, May. 2018, 153-162.
[35] Statsoft, Inc. STATISTICA (data analysis software system), version 7, 2004.
[36] Tavares, S. C. C. de H. Epidemiologia e manejo integrado de Botryodiplodia theobromae - Situação atual no Brasil e no mundo. Fitopatologia Brasileira, Vol. 27, No. s/n, 2002, 46–52.
[37] Terao, D., Nechet, K. de L., and Halfeld-Vieira, B. de A. Competitive and colony layer formation ability as key mechanisms by yeasts for the control Botryosphaeria dothidea fruit rot of mango. Tropical Plant Pathology, Vol. 42, No. 6, Dec. 2017, 451-457
[38] Terao, D., Batista, D. C., e Barbosa, M. A. G. Doenças em pós-colheita de manga. Circular técnica, Vol. s/v, No. 105, Dez. 2013, 1-8.
[39] Vyshali, P., Saraswathi, K.J T., and Mallavarapu, G. R. Chemical composition of the essential oils of Cymbopogon citratus (DC.) stapf grown in three locations in South India. Journal of Essential Oil Bearing Plants, Vol. 18, No. 1, Apr. 2015, 230-235.
[40] Zhang, Z., et al. Antifungal activity of monoterpenes against Botryosphaeria dothidea. Natural Product Communications, Vol. 13, No. 12, Nov. 2018, 1721-1724.
Published
2021-04-06
How to Cite
Ricken Schuelter, A., da Silva, F. O., dos Santos Grignet, R. ., Pereira, D. ., da Silva, R. F. ., Ceccon Merçon Vieira, M. ., Fiorini Rosado, A. ., & Daneluz Gonçalves, V. . (2021). MOLECULAR IDENTIFICATION OF FUNGI ISOLATED FROM POSTHARVEST MANGO FRUITS AND EVALUATION OF ESSENTIAL OILS ACTIVITY OF GROWTH INHIBITION. International Journal of Research -GRANTHAALAYAH, 9(3), 178–189. https://doi.org/10.29121/granthaalayah.v9.i3.2021.3782
Issue
Section
Articles
License
With the licence CC-BY, authors retain the copyright, allowing anyone to download, reuse, re-print, modify, distribute, and/or copy their contribution. The work must be properly attributed to its author.
It is not necessary to ask for further permission from the author or journal board.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
