ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS
DOI:
https://doi.org/10.29121/granthaalayah.v8.i12.2020.2676Keywords:
Antibacterial, Phytochemical, Mango, Ethanol, Aqueous, PseudomonasAbstract [English]
The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.
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References
Bandiola, T. M. (2018). Extraction and Qualitative Phytochemical Screening of Medicinal Plants, International Journal of Pharmacy, 8(1)137-140.
Chukudebe, E. P. Bankole, S. O., Asonibare, A.O & Adedokun, S.A. (2019). Antimicrobial & Phytochemical Properties of Crude Extracts of Some Selected Plants Agents on Some Microbial Isolates, Internal Journal of Agriculture, Environment & Bioresearch 4(5): 2456-8643.
Cheesbrough L., Bard, BM., Parker, TC., and Gould, GW. (2006). District Laboratory Practice in Tropical Countries. Part 2. Published by Cambridge Universities Press. Pp 38-39;64-70. DOI: https://doi.org/10.1017/CBO9780511543470
Doughari, J. H., and Manzara, S. (2008). In vitro Antibacterial Activity of Crude Leaf Extracts of Mangifera indica Linn. African Journalof Microbiology Research 2:67-72.
Dike-Ndudim, J.N., Anyanwu, G.O., Egbuobi, R.C., Okorie, H.M., Udujih, H.I., Nwosu, D.C., and Okolie, N.J.C. (2016). Antibacterial and Phytochemical Potential of Moringa oleifera Leaf Extracts on some Wound and Enteric Pathogenic Bacteria, European Journal of Botany, Plant Sciences and Phytology, 3(1): 50-6
Diso, S.U., M. Ali, S.I. Mukhtar, and Garba, M. (2017). Antibacterial Activity and Phytochemical Screening of Mangifera indica (mango) Stem and Leaf Extracts on Clinical Isolates of Methicillin Resistant Staph aureus, Journal of Advanced Medical and pharmaceutical science. 13 (1):1-6
Garga, M. A., Manga, S. B., Rabah, A. B., Tahir, H., Abdullahi, M., Ahmed, M., Abdullahi, H. A., Bako, I., Abdurrahman, S. A., and Mukhtar, U. F. (2019). Antibacterial Activity and Phytochemical Screening of Moringa oleifera Lam. Leaves and Seeds Extracts on Staphylococcus aureus, International Journal of Research- Graanthaalayah, 7(11): 276-284. https://dio.org/10.5281/zendio.3571982.
Hannan, A., Asghar, S., Naeem, T., Ullah, MI., Ahmed, I., Aneela, S., and Hussain, S. (2013). Antibacterial effect of Mango (Mangifera indica Linn.) leaf extract against antibiotic sensitive and Multi-drug resistant Salmonella typhi, Pakistan Journal of Pharmaceutical Sciences, 26 (4): 715-719
Mathur, A., Singh, R., Yousuf, S., Apraiita, B., Satish, K. (2011). Antifungal Activity of some Plant Extracts Against Clinical Pathogens, Advances in Applied Sciences Research, 2(2): 260-264
Mushore, J., and Matuvhunye T. (2013). Antibacterial Properties of Mangifera indica on Staphylococcus aureus, African Journal of Clinical and Experimental Microbiology, 14(2): 62-74 DOI: https://doi.org/10.4314/ajcem.v14i2.4
Mada SB. Garba, A., Mohammed, HA., Muhammad, A., and Olaguniu AB. (2013). Antimicrobial activity and phytochemical screening of aqueous and ethanol extracts of Momordica charantia L. Leaves, Jounal of Medicinal Plants Researc,. 7(10): 579-586.
Olasenhinde G.I, Sholotan K.J., Openibo J.O, Taiwo O. S., Bello O. A, Ajayi J.B., Ayepola O.O. and Ajayi A.A. (2018). Phytochemical & Antimicrobial properties of Mangifera indica Leaf Extracts, Convenant Journal of Physical & Life Sciences, 6 (1):55-63
Oyeleke, S.B. and Manga, S.B. (2008). Essentials of Laboratory Practicals in Microbiology. Niger,TOBEST PUBLISHER,93-99
Taddaow K., Supap, Saenphet, Yingmance T. & Kanokporn, S. (2018). Antibacterial Effects of Thai Mango (Mangifera indica Linn) Leaves Against Acne-inducing Bacteria, Science International Journal. 30(3) :449-453
Talba, M. A., Suleiman, M. M., Abiola, R., Oniye, S. (2014). Phytochemical Screening and in vitro Antibacterial Activity of Mangifera indica (Mango) Kernel on Aeromonas caviae, IOSR Journal of Pharmacy, 4(10): 45-50. DOI: https://doi.org/10.9790/3013-04010045050
Tollefson, L., and Miller, A. M. (2000). Antibiotic use in Food Animals: Controlling the Human Heath Impact, Journal of AOAC International, 83(2): 245-54 DOI: https://doi.org/10.1093/jaoac/83.2.245
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