CLONING AND EXPRESSION OF VACCINIA VIRUS PROTEIN (B18) IN HETEROLOGOUS HOST

Swaroop Sarkar; V.V.S. Suryanarayana

doi:10.29121/granthaalayah.v5.i8(SE).2017.2249

Authors

Swaroop Sarkar Indian Veterinary Research Institute, Hebbal, Bangalore - 560024, India
V.V.S.Suryanarayana Indian Veterinary Research Institute, Hebbal, Bangalore - 560024, India

DOI:

https://doi.org/10.29121/granthaalayah.v5.i8(SE).2017.2249

Keywords:

Cloning, Expression, Vaccinia Virus B18, FMDV Virulence Enhancer

Abstract [English]

B18 is an immunoglobulin superfamily glycoprotein produced by vaccinia virus which resembles to interferon alpha receptors. It acts both in solution and also when associated with the cell surface. This B18 protein was expressed in heterologous host and purified based on affinity column chromatography. The purified B18 protein has been evaluated for biological activity in Pig Kidney- 15 cells (PK-15) which has been reported to have α –interferon receptors and signaling mechanism. The CPE was more than 90% in the presence of protein and around 50% in the absence. B-18 can enhance the infectivity of FMDV to cells having α –interferon receptors. This provides the basis for developing new strategies for improving attenuated vaccines and its capacity to act as an enhancer protein.

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