• Vagner Rodrigues Santos Associate Professor,Laboratory of Microbiology, Department of Oral Clinica, Oral Pathology, Oral Surgery, Faculty of Dentistry, Universidade Federal de MinasGerais, Belo Horizonte, Brazil
  • Livia SâmaraFranciele de Souza Pinto Dentistry Graduation Student, PROBIC/FAPEMIG Initiation Scientific grants, Faculty of Dentistry, Universidade Federal de MinasGerais, Belo Horizonte, Brazil
  • Bruno César de Borges Araújo e Ribeiro Dentistry Graduation Student, Iniciation Scientific Voluntary Student
  • IsaoKubo Professor of Natural Products Chemistry, Department of Environmental Science, Policy and Management and Department of Nutritional Science and Toxicology, University of California at Berkeley, California, USA




Candida sp, Miconazole, Polygodial, Antifungal Activity, MIC

Abstract [English]

Miconazole has low toxicity, however, there is a high incidence resistance of Candida sp. In the search for new drugs or improve existing ones, the originating products of medicinal plants has been the target of constant studies. The aim of this work was to verifyinvitrosynergismand antifungal activity of miconazole associated with poligodial. Miconazole (MCZ) and Poligodial (P) were dissolved in dimethylsulfoxide (DMSO) 1% done successive dilutions of each product ranging from 25mg to 0,19mg/mL (Polygodial)(10-1 to 10-9mg / ml); 14mg to 0,10mg/mL (miconazole). Antifungal test were based by CLSI diffusion agar against C. albicans (ATCC 18804), C. albicans (LMB01) and C. albicans (LMB02), C. tropicalis (ATCC 18807),C. lusitaniae (ATCC 42720), C. krusei (ATCC 20405).  The results had shown all compounds that were effective in inhibiting Candida species. However, the zones of inhibition in agar diffusion test were higher for Miconazole (16,83mm) and Polygodial (15,16mm) while the MIX showed lower inhibition zones (14,43mm) when compared with controls. MIX was more effective in MIC test, and lowest concentration were at 10-6 dilution (0,60mg / ml) compared to MCZ and P controls.


Download data is not yet available.


Singh A., R. Verna and A. Agrawal, J. Oral. Maxillofac. Pathol.18, 2014, S81 - 85. DOI: https://doi.org/10.4103/0973-029X.141325

Collins C.D., S. Cookinham and J. Smith, Patient Preferency Adherence. 5, 2011, 369-374. DOI: https://doi.org/10.2147/PPA.S14047

Hebecker B., Naglik JR, Hube B and Jacobsen ID. Expert. Review Anti-Infectuous. Therapy.12, 2014, 867-879. DOI: https://doi.org/10.1586/14787210.2014.916210

Noronha V.R.A.S., G.S. Araujo, R.T. Gomes, S.H. Iwanaga, M.C. Barbosa, E.N. Abdo, E. Ferreira e Ferreira, A.C. V. Campos, A.A. Souza, S.R. Abreuand V.R. Santos, Current Clinical Pharmacology. 9, 2014, 359 -364. DOI: https://doi.org/10.2174/1574884709666140205210051

Gaitán-Cepeda L.A., O. Sánchez-Vargas and N. Castillo, International Journal STD AIDS. 26, 2015, 625-632. DOI: https://doi.org/10.1177/0956462414548906

Owotade F.J, M. Patel. Oral Surgery Oral Medicine Oral Pathology Oral Radiology. 118, 2014, 455- 460. DOI: https://doi.org/10.1016/j.oooo.2014.07.004

Coronado-Castellote L., and Y. Jiménez-Soriano. Journal ClinicalExperimental Dentistry.5, 2013, e279 -e286. DOI: https://doi.org/10.4317/jced.51242

Costa E., S. Silva, F. Tavaria, M. Pintado. Pathogens. 3, 2014, 908 -919. DOI: https://doi.org/10.3390/pathogens3040908

Gebremedhin S., B. Dorocka-Bobkowska, M. Prylinski, K. Konopka, and N. Duzgunes. Journal of Physiology and Pharmacology. 65, 2014, 593-600.

Hu Z., J. Zhang, and X. Cheng. Pharmacology Biology. 53, 2015, 251. DOI: https://doi.org/10.3109/13880209.2014.914232

MetugriachukY., O. Kuroi, K. Pavasuthipaisit, J. Tsuchiya, E. Minelli, R. Okura, E. Fesce, and F. Marotta. Chinese Journal of Digestive Diseases. 6, 2005, 98 -103. DOI: https://doi.org/10.1111/j.1443-9573.2005.00199.x

PiérardG., T. Hermanns-Lê, Delvenne P, and C. Piérard-Franchimont, Expert Opinion Pharmacotherapy. 13, 2012, 1187 -1194 . DOI: https://doi.org/10.1517/14656566.2012.687047

Wong-Paz J.E., J.C. Contreras-Esquivel, R. Rodríguez-Herrera, M.L. Carrillo-Inungaray, L.I. López, G.V. Nevárez-Moorillón, and C.N. Aguilar. Asian Pacific Journal of Tropical Medicine.8, 2015, 104 -111. DOI: https://doi.org/10.1016/S1995-7645(14)60299-6

Lalla R.V., L.L. Patton, and A. Dongari-Bagtzoglou. Journal of Californian Dental Association. 41, 2013, 263-268.

Garcia-Cuesta C., M.G. Sarrion-Pérez, and J.V. Bagán. Journal of Clinical Experimental Dentistry. 6, 2014, 576-582. DOI: https://doi.org/10.4317/jced.51798

Almeida T., F. Ribeiro, H. Kloos, and C. Zani. Memoria do Instituto Oswaldo Cruz. 96, 2001, 831-833. DOI: https://doi.org/10.1590/S0074-02762001000600016

Malheiros A., V. CechinelFilho, C.B. Schmitt, R.A. Yunes, A. Escalante, L. Svetaz, S. Zacchino, and F. DelleMonache. Journal of Pharmacyand Pharmaceutical Sciences. 8, 2005, 335 - 339.

MecchiM.C. and J.H. Lago. Nat Prod Res. 27, 2013, 1927 -1929. DOI: https://doi.org/10.1080/14786419.2013.782491

Derita M., I. Montenegro, F. Garibotto, R.D. Enriz, M.C. Fritis, and S.A. Zacchino. Molecules. 18, 2013, 2029-2051. DOI: https://doi.org/10.3390/molecules18022029

Kubo I., K. Fujita, and S.H. Lee. Journal of Agricultural Food and Chemistry. 49, 2001, 1607-1611. DOI: https://doi.org/10.1021/jf000136g

Kubo I., K. Fujita, S.H. Lee, and T.J. Ha. Phytotherapy Research. 19, 2005, 1013- 1017. DOI: https://doi.org/10.1002/ptr.1777

McCallion R.F., A.L. Cole, J.R. Walker, J.W. Blunt and M.H. Munro, Planta Medica.44, 1982, 134-138. DOI: https://doi.org/10.1055/s-2007-971422

Leonard CM. and AM Viljoen. Journal of Ethnopharmacolology. 165, 2015, 260-285- . DOI: https://doi.org/10.1016/j.jep.2015.02.021

Lee S., J.R. Lee, C. Lunde, and I. Kubo. Planta Medica. 65, 21999, 204-208. DOI: https://doi.org/10.1055/s-1999-13981

Fujita K. and I. Kubo. Bioorganic Medicinal Chemistry. 13, 2005, 6742 -6747. DOI: https://doi.org/10.1016/j.bmc.2005.07.023

De Luca M.P., J.R. Franca, F.A. Macedo, L. Grenho, M.E. Cortes, A.A. Faraco, A.N. Moreira, and V.R. Santos. Biomedicine Research International. 2014,

<https://www.hindawi.com/journals/bmri/2014/348647/>doi: 10.1155/2014/348647,08 p. DOI: https://doi.org/10.1155/2014/348647

Franca J.R., M.P. De Luca , T.G. Ribeiro, R.O. Castilho, A.N. Moreira, V.R. Santos, and A.A. Faraco. BMC Complementary and Alternative Medicine. 14, 2014, 478, 11 p. DOI: https://doi.org/10.1186/1472-6882-14-478

Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. In twentieth informational supplement, CLSI document M 100-S20 (Wayne, PA, USA 2010), 238p..

Lunde C.S., and I. Kubo. Antimicrobial Agents Chemotherapy. 44, 2000, 1943-1953. DOI: https://doi.org/10.1128/AAC.44.7.1943-1953.2000

De Cremer K., E. Lanckacker, T.L. Cools, M. Bax, K. de Brucker, P. Cos, B.P. Cammue, and K. Thevissen. Antimicrobial Agents Chemotherapy. 59, 2015, 421-426. DOI: https://doi.org/10.1128/AAC.04229-14

Wei G.X. and L.A Bobek. Journal of Antimicrobial Chemotherapy. 53, 2004, 750-758. DOI: https://doi.org/10.1093/jac/dkh181

Sheikh S., D. Gupta, S. Pallagatti, I. Singla, R. Gupta, and V. Goel. New York State Dental Journal. 79, 2013, 58-64.

Scodavolpe S., S. Quaranta, B. Lacarelle, and C. Solas. Annals of Biology and Clinics 72, 2014, 391-404. DOI: https://doi.org/10.1684/abc.2014.0968

Koletar S.L., in Diagnostic Microbiology, edited by C.R.Mahon, G. ManuselisJr Saunders, Phyladelfia, 1995.

Tian J., X. Ban, H. Zeng, J. He, Y. Chen, and Y. Wang. PLoS One. 7, 2012, e30147, 10p.. DOI: https://doi.org/10.1371/journal.pone.0030147

Gordaliza M.. Mar Drugs. 10, 2012, 358 -402. DOI: https://doi.org/10.3390/md10020358

Campbell B.C., K.L. Chan, and J.H. Kim. Frontiers of Microbiology. 3, 2012, 79. DOI: https://doi.org/10.3389/fmicb.2012.00079

MoyeenulHuq A. K. M., J.A. Jamal, and J. Stanslas. Evidence Based Complementary and Alternative Medicine. 2014, 11 p.

https://www.hindawi.com/journals/ecam/2014/782830/>doi: 10.1155/2014/782830. DOI: https://doi.org/10.1155/2014/782830

Figure 3. http://pharmacologycorner.com/study-aids-for-antifungals-pharmacology-diagram-showing-moa-and-table-summary-for-activity-spectrum/, November 12 2017)




How to Cite

Santos, V. R., de Souza Pinto, L. S., de Borges, B. C., & Kubo, I. (2017). DRUG ENHANCEMENT, SYNERGISM AND ANTIFUNGAL ACTIVITY OF MICONAZOLE ASSOCIATED POLYGODIAL AGAINST CANDIDA ALBICANS. International Journal of Research -GRANTHAALAYAH, 5(11), 95–101. https://doi.org/10.29121/granthaalayah.v5.i11.2017.2332