DEVELOPMENT OF A COMPOSITE OF POLY-Ɛ-CAPROLACTONE-CERIUM OXIDE

Alejandra Melissa; Simón Yobanny

doi:10.29121/granthaalayah.v5.i7.2017.2158

Authors

Alejandra Melissa Martínez-Hernández Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas Universidad Autónoma de Ciudad Juárez, México
Simón Yobanny Reyes-López Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas Universidad Autónoma de Ciudad Juárez, México

DOI:

https://doi.org/10.29121/granthaalayah.v5.i7.2017.2158

Keywords:

Electrospinning, Fibers, Oxide de Cerium, Poly-Ɛ-Caprolactone, Comet Assay

Abstract [English]

Nanotechnology is used in a variety of sectors for the manufacture of materials. However, there is a risk from exposure to nanomaterials due to their chemical composition and processing form, giving rise to health risks. CeO2 particles present antioxidant properties that accelerate the wound poly-Ɛ-caprolactone (PCL) provides the property of biocompatibility and biodegradability of the material. For the regulation of the transport and release of cerium oxide particles was incorporated into a polymeric matrix composed of PCL. Therefore, in this study we propose the elaboration of a composite material of poly-Ɛ-caprolactone-cerium by the electrospinning technique. Electrospinning for example has been widely used to fabricate scaffolds imitating the structure of natural extracellular matrix. The fibers obtained with an average diameter of 600 nm was characterized by confocal microscopy, scanning electron microscopy, infrared spectroscopy, Differential thermal analysis, Thermogravimetric Analysis and Differential Scanning Calorimetry. Additionally, genotoxic effect was evaluated by comet assay in Vicia faba cell nuclei, results show that the material made has no genotoxic effects in Vicia faba cells.

Downloads

Download data is not yet available.

References

Fang, J., Wang, X., & Lin, T. (2011). Functional applications of electrospun nanofibers, Nanofibers-Production, Properties and Functional Applications. In Nanofibers-production, properties and functional applications. InTech. 1-458. DOI: https://doi.org/10.5772/24998

López-Esparza, J., Espinosa-Cristóbal, L. F., Donohue-Cornejo, A., & Reyes-López, S. Y. (2016). Antimicrobial activity of silver nanoparticles in polycaprolactone nanofibers against gram-positive and gram-negative bacteria. Industrial & Engineering Chemistry Research, 55(49), 12532-12538. DOI: https://doi.org/10.1021/acs.iecr.6b02300

Garibay-Alvarado, J. A., Espinosa-Cristóbal, L. F., & Yobanny, S. Fibrpus Silica/Hydroxyapatite Composite by Electrospinning.

Frenot, A. y Chronakis, I.S. 2003. Polymer nanofibers assembled by electrospinning. ELSEVIER. 8:64-75. DOI: https://doi.org/10.1016/S1359-0294(03)00004-9

Lord, M. S.; Tsoi, B.; Gunawan, C.; Yang, W.; Amal, R. y Whitelock, M. 2013. Anti-angiogenic activity of heparin functionalized cerium oxide nanoparticles. Biomaterials. 34: 8808-8818. DOI: https://doi.org/10.1016/j.biomaterials.2013.07.083

R. Nirmala, H. S. Kang, H. M. Park, R. Navamathavan, I. S. Jeong, H. Y. Kim, Silver-loaded biomimetic hydroxyapatite grafted poly (ε-caprolactone) composite nanofibers: a cytotoxicity study, J. Biomed. Nanotechnol. 8 (2012) 125-132. DOI: https://doi.org/10.1166/jbn.2012.1359

S. Y. Reyes López, D. Cornejo Monroy, G. González García, A novel route for the preparation of gold nanoparticles in polycaprolactone nanofibers, J. Nanomater. (2015). DOI: https://doi.org/10.1155/2015/485121

J. H. Roque-Ruiz, E. A. Cabrera-Ontiveros, J. Torres Pérez, S. Y. Reyes-López, Preparation of PCL/Clay and PVA/Clay electrospun fibers for Cadmium (Cd+2), Chromium (Cr+3), Copper (Cu+2) and Lead (Pb+2) removal from water, Water Air Soil Pollut. 227:286 (2016) 1-17.

Collins, A, R. 2004. The comet assay for DNA damage and repair. Molecular biotechnology. 26(3): 249-261. DOI: https://doi.org/10.1385/MB:26:3:249

Liao, W.; McNutt, M. A.; Zhu, W. 2009. The comet assay: A sensitive method for detecting DNA damage in individual cells Methods. 48(1): 46-53. DOI: https://doi.org/10.1016/j.ymeth.2009.02.016

Ramírez, R. P.; Mendoza, C. A. Ensayos toxicológicos para la evaluación de sustancias químicas en agua y suelo. Secretaria de Medio ambiente y Recursos Naturales (SEMARNAT). México. 2008. 235-236p.

Gichner, T. 2003. Comet Assay in higher plants. Bioassays in plant Cells for Improvemente of Ecosystem and Human Health. Katowice: Wydawnictwo Uniwersytetu Śląskiego, 123-131.

Hernández, E. J.; Silva, R. R.; García A. R.; García, A. A.; Edward, H. B.; Cárdenas, G. G. y Cueto, H. A. 2012. Synthesis and Physico-chemical Characterization of CeO2/ZrO2-SO4. J. Mex. Chem. Soc. 56(2): 115-120.

CIAT. Los surfractantes: Clases, propiedades y uso con herbicidas. Edit. XYZ. Colombia. 1980. 48 p.

Sill, T.; Horst, A. y Recum, V. 2008. Review electrospinning: Application in drug delivery and tissue engineering. ScienceDirect. 29(13): 1989-2006.

Moreno, B. R. Reología de suspensiones cerámicas. Edit. CSIC. España. 2005. 325p.

Salager, J.; Anton, R. Métodos de medición de la tensión superficial o interfacial. Edit. FRP. Mérida. 2005. 20p.

Ansari, A. A. 2010. Optical and structural properties of sol-gel derived nanostructured CeO2. Journal of semiconductors. 31(5): 1-5. DOI: https://doi.org/10.1088/1674-4926/31/5/053001

Ghasemi, M. L.; Prabhakaran, P. M.; Morshed, M.; Hossein, N. M. y Ramakrishna, S. 2010 Bio- functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Materials Science and Engineering C. 30(8): 1129-1136. DOI: https://doi.org/10.1016/j.msec.2010.06.004

Leilei, X.; Huanling, S.; Lingjun, Ch. 2012. Mesoporous nanocrystalline ceria-zirconia solid solutions supported nickel based catalysts for CO2 reforming of CH4. SciVerse ScienceDirect. 37 (23): 18001-18020.

Garcia, H. P.; Pabón, A.; Arias, C.; Blair, S. 2013. Evaluación del efecto citotóxico y del daño genético de extractos estandarizados de Solanum nudum con actividad anti-Plasmodium. 33 (1): 78-87. DOI: https://doi.org/10.7705/biomedica.v33i1.838