SUPPLEMENTATION OF SPIRULINA AND VITAMIN C ATTENUATED THE NEPHROTOXICITY INDUCED BY CISPLATIN ADMINISTRATION
DOI:
https://doi.org/10.29121/granthaalayah.v4.i1.2016.2847Keywords:
Science, Nephrotoxicity, SpirulinaAbstract [English]
Overexposure of the kidneys to cisplatin [Cisplatin (cis- diaminedichloroplatinum II) (CDDP)] and cisplatin complexes cause degradation of the renal cells, and eventually lead to renal failure. Agents like antioxidants, modulators of nitric oxide (NO) and anti-apoptotic molecules are reported to reduce nephrotoxicity. In the present study we tested the hypothesis that supplementation of antioxidant spirulina and vitamin c reduces cisplatin induced nephrotoxicity. The study was carried out on female Balb /C mice of the following five groups: control (i); cisplatin (ii); vitamin c (iii); spirulina (iv); and vitamin c + spirulina + cisplatin (v). Plasma urea and creatinine levels were estimated 3 and 6 days after the injection of cisplatin and uptake studies with 99m Technician-ethylene dicysteine was performed to assess the renal clearance rate. Kidneys were isolated and processed for electron microscopy and light microscopy. The activity and concentration of antioxidant enzymes such as glutathione, catalase, superoxide dismutase and GST was also determined. Dual supplementation of spirulina and vitamin c significantly protected cisplatin-induced increased levels of serum urea, creatinine, improved renal clearance rate, the declined renal antioxidant enzymes and increased LPO levels. Hence, we conclude that supplementation of spirulina and vitamin c had a beneficial effect on cisplatin induced nephrotoxicity in mice.
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Ajith, T. A., Usha, S. and Nivitha, V. (2007). Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinicachimicaacta; international journal of clinical chemistry 375, 82-86. DOI: https://doi.org/10.1016/j.cca.2006.06.011
Antunes, L. M., Araujo, M. C., Darin, J. D. and Bianchi, M. L. (2000). Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis in Wistar rat bone marrow cells. Mutat Res 465, 131-137. DOI: https://doi.org/10.1016/S1383-5718(99)00220-X
Bhattacharyya, S. and Mehta, P. (2012). The hepatoprotective potential of Spirulina and vitamin C supplemention in cisplatin toxicity. Food & function 3, 164-169. DOI: https://doi.org/10.1039/C1FO10172B
Boyde, T. R. and Rahmatullah, M. (1980). Optimization of conditions for the colorimetric determination of citrulline, using diacetyl monoxime. Anal Biochem 107, 424-431. DOI: https://doi.org/10.1016/0003-2697(80)90404-2
Camano, S., Lazaro, A., Moreno-Gordaliza, E., Torres, A. M., de Lucas, C., Humanes, B., Lazaro, J. A., Milagros Gomez-Gomez, M., Bosca, L. and Tejedor, A. (2010). Cilastatin attenuates cisplatin-induced proximal tubular cell damage. The Journal of pharmacology and experimental therapeutics 334, 419-429. DOI: https://doi.org/10.1124/jpet.110.165779
Cetin, R., Devrim, E., Kilicoglu, B., Avci, A., Candir, O. and Durak, I. (2006). Cisplatin impairs antioxidant system and causes oxidation in rat kidney tissues: possible protective roles of natural antioxidant foods. J ApplToxicol 26, 42-46.
Chattopadhyaya I, Gupta S, Mohammed A, Mushtaq N, Chauhan S, Ghosh S. (2015). Neuroprotective effect of Spirulina fusiform and amantadine in the 6-OHDA induced Parkinsonism in rats. BMC Complement Altern Med 15,296.
Dillioglugil, M. O., MaralKir, H., Gulkac, M. D., OzonKanli, A., Ozdogan, H. K., Acar, O. and Dillioglugil, O. (2005). Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urologiainternationalis 75, 340-344. DOI: https://doi.org/10.1159/000089171
Dizdar, O., Demirci, U. and Ozcan, M. F. (2011). "Unfit" for Cisplatin: on basis of which GFR? Journal of clinical oncology : official journal of the American Society of Clinical Oncology 29, 3945. DOI: https://doi.org/10.1200/JCO.2011.37.6640
Gad, A. S., Khadrawy, Y. A., El-Nekeety, A. A., Mohamed, S. R., Hassan, N. S. and Abdel-Wahhab, M. A. (2011). Antioxidant activity and hepatoprotective effects of whey protein and Spirulina in rats. Nutrition 27, 582-589. DOI: https://doi.org/10.1016/j.nut.2010.04.002
Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J BiolChem 249, 7130-7139.
Jones, T. W., Chopra, S., Kaufman, J. S., Flamenbaum, W. and Trump, B. F. (1985). Cis-diamminedichloroplatinum (II)-induced acute renal failure in the rat. Correlation of structural and functional alterations. Lab Invest 52, 363-374.
Jordan, P. and Carmo-Fonseca, M. (2000). Molecular mechanisms involved in cisplatin cytotoxicity. Cell Mol Life Sci 57, 1229-1235. DOI: https://doi.org/10.1007/PL00000762
Jung, M., Hotter, G., Vinas, J. L. and Sola, A. (2009). Cisplatin upregulates mitochondrial nitric oxide synthase and peroxynitrite formation to promote renal injury. Toxicology and applied pharmacology 234, 236-246. DOI: https://doi.org/10.1016/j.taap.2008.10.014
Jung, S. H., Kim, H. J., Oh, G. S., Shen, A., Lee, S., Choe, S. K., Park, R. and So, H. S. (2014). Capsaicin Ameliorates Cisplatin-Induced Renal Injury through Induction of Heme Oxygenase-1. Molecules and cells 37, 234-240. DOI: https://doi.org/10.14348/molcells.2014.2322
Kabasakal, L., Atay, S., Vural, V. A., Ozker, K., Sonmezoglu, K., Demir, M., Uslu, I., Isitman, A. T. and Onsel, C. (1995). Evaluation of technetium-99m-ethylenedicysteine in renal disorders and determination of extraction ratio. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 36, 1398-1403.
Kanwar, Y. S. and Rosenzweig, L. J. (1982). Altered glomerular permeability as a result of focal detachment of the visceral epithelium. Kidney international 21, 565-574. DOI: https://doi.org/10.1038/ki.1982.63
Khan, M., Shobha, J. C., Mohan, I. K., Rao Naidu, M. U., Prayag, A. and Kutala, V. K. (2006). Spirulina attenuates cyclosporine-induced nephrotoxicity in rats. J ApplToxicol 26, 444-451. DOI: https://doi.org/10.1002/jat.1159
Kono, Y. (1978). Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch BiochemBiophys 186, 189-195. DOI: https://doi.org/10.1016/0003-9861(78)90479-4
Kulshreshtha, A., Zacharia, A. J., Jarouliya, U., Bhadauriya, P., Prasad, G. B. and Bisen, P. S. (2008). Spirulina in health care management. Curr Pharm Biotechnol 9, 400-405. DOI: https://doi.org/10.2174/138920108785915111
Kuriakose, G. C. and Kurup, M. G. (2011). Antioxidant and antihepatotoxic effect of Spirulina laxissima against carbon tetrachloride induced hepatotoxicity in rats. Food & function 2, 190-196. DOI: https://doi.org/10.1039/c0fo00163e
Laurens, W., Battaglia, C., Foglieni, C., De Vos, R., Malanchini, B., Van Damme, B., Vanrenterghem, Y., Remuzzi, G. and Remuzzi, A. (1995). Direct podocyte damage in the single nephron leads to albuminuria in vivo. Kidney international 47, 1078-1086. DOI: https://doi.org/10.1038/ki.1995.154
Luck, H. (1957). [Inactivation of catalase by hydrogen peroxide. I. Studies on the maximum amount of hydrogen peroxide cleavable by the enzyme]. BiochemischeZeitschrift 329, 165-174.
Martínez-Galero E, Pérez-Pastén R, Perez-Juarez A, Fabila-Castillo L, Gutiérrez-Salmeán G, Chamorro G (2015). Preclinical antitoxic properties of Spirulina (Arthrospira). Pharm Biol. 2015 Oct 6:1-9. [Epub ahead of print]
Meyer, K. B. and Madias, N. E. (1994). Cisplatin nephrotoxicity. Mineral and electrolyte metabolism 20, 201-213.
Moran, J. K. (1999). Technetium-99m-EC and other potential new agents in renal nuclear medicine. Seminars in nuclear medicine 29, 91-101. DOI: https://doi.org/10.1016/S0001-2998(99)80002-6
Moron, M. S., Depierre, J. W. and Mannervik, B. (1979). Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. BiochimBiophysActa 582, 67-78. DOI: https://doi.org/10.1016/0304-4165(79)90289-7
Pentón-Rol G, Lagumersindez-Denis N, Muzio L, Bergami A, Furlan R, Fernández-Massó JR, Nazabal-Galvez M, Llópiz-Arzuaga A, Herrera-Rolo T, Veliz-Rodriguez T, Polentarutti N, Marín-Prida J, Raíces-Cruz I,Valenzuela-Silva C, Teixeira MM, Pentón-Arias E (2015). Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice. J NeuroimmunePharmacol. Nov 10. [Epub ahead of print]
Ponce-Canchihuaman, J. C., Perez-Mendez, O., Hernandez-Munoz, R., Torres-Duran, P. V. and Juarez-Oropeza, M. A. (2010). Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids in health and disease 9, 35. DOI: https://doi.org/10.1186/1476-511X-9-35
Premkumar, K., Abraham, S. K., Santhiya, S. T. and Ramesh, A. (2004). Protective effect of Spirulina fusiformis on chemical-induced genotoxicity in mice. Fitoterapia 75, 24-31. DOI: https://doi.org/10.1016/j.fitote.2003.07.008
Raddassi, K., Berthon, B., Petit, J. F. and Lemaire, G. (1994). Role of calcium in the activation of mouse peritoneal macrophages: induction of NO synthase by calcium ionophores and thapsigargin. Cellular immunology 153, 443-455. DOI: https://doi.org/10.1006/cimm.1994.1041
Ray, S., Roy, K. and Sengupta, C. (2007). In vitro evaluation of protective effects of ascorbic acid and water extract of Spirulina plantesis (blue green algae) on 5-fluorouracil-induced lipid peroxidation. Actapoloniaepharmaceutica 64, 335-344.
Reddy, V. G., Khanna, N. and Singh, N. (2001). Vitamin C augments chemotherapeutic response of cervical carcinoma HeLa cells by stabilizing P53. BiochemBiophys Res Commun 282, 409-415. DOI: https://doi.org/10.1006/bbrc.2001.4593
Seaton, B. and Ali, A. (1984). Simplified manual high performance clinical chemistry methods for developing countries. Medical laboratory sciences 41, 327-336.
Selmi, C., Leung, P. S., Fischer, L., German, B., Yang, C. Y., Kenny, T. P., Cysewski, G. R. and Gershwin, M. E. (2011). The effects of Spirulina on anemia and immune function in senior citizens. Cellular & molecular immunology 8, 248-254. DOI: https://doi.org/10.1038/cmi.2010.76
Sohini and Rana, S. V. (2007). Protective effect of ascorbic acid against oxidative stress induced by inorganic arsenic in liver and kidney of rat. Indian journal of experimental biology 45, 371-375.
Trush, M. A., Mimnaugh, E. G., Ginsburg, E. and Gram, T. E. (1981). In vitro stimulation by paraquat of reactive oxygen-mediated lipid peroxidation in rat lung microsomes. Toxicology and applied pharmacology 60, 279-286. DOI: https://doi.org/10.1016/0041-008X(91)90231-3
Vazquez-Sanchez, J., Ramon-Gallegos, E., Mojica-Villegas, A., Madrigal-Bujaidar, E., Perez-Pasten-Borja, R. and Chamorro-Cevallos, G. (2009). Spirulina maxima and its protein extract protect against hydroxyurea-teratogenic insult in mice. Food ChemToxicol 47, 2785-2789. DOI: https://doi.org/10.1016/j.fct.2009.08.013
Weisburger, J. H. (2002). Lycopene and tomato products in health promotion. ExpBiol Med (Maywood) 227, 924-927. DOI: https://doi.org/10.1177/153537020222701014
Yakoot, M. and Salem, A. (2012). Spirulina platensis versus silymarin in the treatment of chronic hepatitis C virus infection. A pilot randomized, comparative clinical trial. BMC gastroenterology 12, 32.
Yousef, M. I., Saad, A. A. and El-Shennawy, L. K. (2009). Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food ChemToxicol 47, 1176-1183. DOI: https://doi.org/10.1016/j.fct.2009.02.007
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