BLACK TEA INFUSION AMELIORATES ENZYMATIC CHANGES INDUCED BY SUBCUTANEOUS EXPOSURE OF GASOLINE AND GM-10 IN MICE
Keywords:Gasoline, Gm-10, Black Tea Extract, Kidney
The present study was carried out to examine the ameliorative effect of black tea infusion on gasoline and GM-10 induced enzymatic changes in kidney of mice. Eighty healthy adult Swiss strain male albino mice weighing 32-35 gm were divided into eight groups including untreated control and various treated groups. Treated groups were subcutaneously administered with gasoline (412 mg/kg/day) and GM-10 low dose (206 mg/kg/day) and high dose (412 mg/kg/day) for 30 days. Black tea infusion (2%) was orally administered alone and along with gasoline and GM-10 through drinking water. All experimental animals were sacrificed on 31st day by cervical dislocation; kidney were isolated and used. Activities of succinic dehydrogenase, adenosine triphosphatase, acid phosphatase and alkaline phosphatase were assayed in kidney.The results revealed that subcutaneous administration of gasoline and GM-10 caused dose-dependent, significant enzymatic alterations in kidney of mice. Oral administration of black tea infusion along with subcutaneous treatment with gasoline and GM-10 significantly ameliorates all enzymatic changes induced by gasoline and GM-10 in kidney of mice.
Beatty CH, Basinger GM, Dully CC, Bocek RM. 1966.Comparison of red and white voluntary skeletal muscle of several species of primates. J Histochem Cytochem. 14: 590-600. DOI: https://doi.org/10.1177/14.8.590
Bessey OA, Lowry OH, Brick NJ. 1946. A method for the rapid determination of acid and alkaline phosphatase in 5 cu mm of serum. J Biol Chem. 164: 321. DOI: https://doi.org/10.1016/S0021-9258(18)43072-4
Castranova V, Rabovsky J, Tucker JH, Miles PR. 1988. The alveolar type ii epithelial cell : a multifunctional pneumocyte. Toxicol Appl Pharmacol. 93: 472-480. DOI: https://doi.org/10.1016/0041-008X(88)90051-8
Du Toit R, Volsteedt Y, Apostolides Z. 2001. Comparision of the antioxidant content of fruits, vegetables and teas measured as vitamin c equivalents. Toxicology. 166: 63-69.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin-phenol reagent. J Biol Chem. 198: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Quinn PJ, White IG. 1968. Distribution of adenosine triphosphatase activity in ram and bull spermatozoa. J Redrod Fertil. 15: 449-452. DOI: https://doi.org/10.1530/jrf.0.0150449
Yang CS, Maliakal P, Meng X. 2002. Inhibition of carcinogenesis by tea. Ann Rev Pharmacol Toxicol. 42: 25-54. DOI: https://doi.org/10.1146/annurev.pharmtox.42.082101.154309
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