COMPARATIVE ANALYSIS OF ANTIOXIDANT PROFILES OF BARK, LEAVES AND SEEDS OF SYZYGIUM CUMINI (INDIAN BLACKBERRY)

Authors

  • RabbeyaHaroon Department of Chemistry, Forman Christian College, Lahore 54600, PAKISTAN
  • Dr. SeemalJelani Department of Chemistry, Forman Christian College, Lahore 54600, PAKISTAN
  • FakihaKomalArshad Department of Chemistry, Forman Christian College, Lahore 54600, PAKISTAN

DOI:

https://doi.org/10.29121/granthaalayah.v3.i5.2015.3010

Keywords:

Antioxidant, Syzygiumcumini, Bark, Leaves, Seeds, Phenolic, Flavonoid

Abstract [English]

Syzygiumcumini plant is well known for its medicinal values. This study focuses on the comparative antioxidative capacity of bark, leaves and seeds of Syzygiumcumini plant.Several fractions were made from the crude methanolic extract of bark, leaves and seeds in n-hexane, chloroform, ethyl acetate and butanol and aqueous.Leaves aqueous fraction showed highest phenolic content of 253.6 ± 0.025mg/mL while seed aqueous showed maximum flavonoid content of 0.732 ± 0.0005 mg/mL among all extracts. Bark Ethyl acetate fraction via reducing power assay showed absorbance 3.27 ± 0.19 abs and methanolic and Ethyl acetate fraction of leaves via phosphomolybdate assay showed maximum reducing strength of 15.97 ± 0.008 and 15.97 ± 0.037 mg/mL respectively among all. Seed Chloroform fraction showed maximum TEAC value of 6.28 whereas, Ethyl acetate leaves fraction has shown maximum DPPH inhibition. Where, Hexane fraction of bark showed maximum antioxidant consumption against peroxyl radical at 1.24 at 500 nm among all.The results of the assays showed that, in general, all the extracts of Syzygiumcuminiposses considerable antioxidant and radical scavenging properties, however, polar fractions of all three samples i.e., aqueous and ethyl acetate  have exhibited a remarkable antioxidant behavior in all the antioxidant assays.

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References

Peschel W.; Sanchez-Rabaneda, F.; Dieck,W.; Plesher A.; Gartzia,I. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chemistry, 2006, 97, 137-150 DOI: https://doi.org/10.1016/j.foodchem.2005.03.033

Newman, D. J.; Cragg, G.M.; Snader, K.M. Natural products as a source of new drugs over the period 1981-2002. Journal of Natural Products. 2003, 66(7), 1002-1037.

KunchaJayachandra.; Sharmila Devi, V.; In-vitro Antioxidant activity of Methanolic Extract of Syzygiumcumini Linn. Bark, Asian journal of biomedical and pharmaceutical sciences, 2011, pp 45.

KunchaJayachandra.; AyyachamyMaheswaran.; MariappanMurali. In- vitro evaluation of nitric oxide scavenging activity of the methanolic and aqueous extract of Syzygiumcumini bark (Myrtaceae). International journal of pharmaceutical sciences and research, 2012, 3, 01-05.

Muruganandan, S.; Srinivasan, K.; Chandra, S.; Tandan, S.K. Anti-inflammatory activity of Syzygiumcumini bark. Fitoterapia.2001, 72(4), 369–375.

Jayamohan, N.S.; Sharma Bhargavi.; Pawan Kumar Prajapati.; KunchaJayachandra. Evaluation of In vitro Antioxidant Potential and Screening of Anthelmintic Activity of Methanolic Extract of Syzygiumcumini Bark (Myrtaceae).Afr J Pharm Pharacol 2011; 5 (7): 948-956.

Kavitha, K.; Murali, M.; Jayachandra, K. Preliminary Phytochemical Screening, Anthelmintic Activity of Methanolic and Aqueous Extract of Syzygiumcumini Linn. Bark (Myrtaceae), J. Pharm. Sci. & Res. 2011, 3(9), 1460-1465

Burkill, HM .The Useful Plant of West Tropical Africa.Royal Botanic Garden Kew. Vol. 4, 1997, p. 253.

Zhi Ping Ruan.; Liang Zhang.; Yi Ming Lin. Evaluation of the Antioxidant Activity of Syzygiumcumini Leaves. Molecules 2008, 13(10), 2545- DOI: https://doi.org/10.3390/molecules13102545

ShyamalaGowri and K. Vasantha, Phytochemical Screening and Antibacterial Activity of Syzygiumcumini (L.) (Myrtaceae) Leaves Extracts, International Journal of PharmTech Research. 2010, 2(2), 1569-1573

Jain A.; Sharma S.; Goyal M.; Dubey S.; Jain S.; Sahu J.; Sharma A.; Kaushik A. Anti-inflammatory activity of Syzygiumcumini leaves. International Journal of Phytomedicine. 2010, 2(2), 124-126.

De Bona, K.S.; Bellé, L.P.; Bittencourt, P.E.; Bonfanti, G. Cargnelluti, L.O.; Pimentel, V.C.; Ruviaro, A.R.; Schetinger, M.R.; Emanuelli, T.; Moretto, M.B. Erythrocytic enzymes and antioxidant status in people with type 2 diabetes: beneficial effect of SyzygiumcuminileAf extract in vitro. Diabetes Res ClinPract. 2011, 94(1), 84-90.

Bajpai M.; Pande A.; Tewari SK.; Prakash D. Phenolic contents and antioxidant activity of some food and medicinal plants. Int. J. Food. Sci. Nutr. 2005, 56(4), 287-291 DOI: https://doi.org/10.1080/09637480500146606

Aqil, F.; Gupta, A.; Munagala, R.; Jeyabalan, J.; Kausar, H.; Sharma, R.J.; Singh, I.P.; Gupta, R.C. Antioxidant and anti proliferative activities of anthocyanin/ellagitannin-enriched extracts from Syzygiumcumini L. (Jamun, the Indian Blackberry). Nutr Cancer. 2012, 64(3), 428-38 DOI: https://doi.org/10.1080/01635581.2012.657766

Karthic, K.; Kirthiram, K.S.; Sadasivam, S.; Thayumanavan, B.; Palvannan, T. Identification of alpha-amylase inhibitors from Syzygiumcuminilinn Seeds. Indian Journal of Experimental Biology. 2008, 46, 677-680

Shinde, Jayantrao.; Taldone, Tony.; Barletta, Michael.; Kunaparaju, Naveen.;Hu, Bo.;Kumar, Sunil.; Placido, Jessica.; Zito, S William. Alpha-glucosidase inhibitory activity of Syzygiumcumini (Linn.)Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats. Carbohydrate Research. 2008, 19, 343(7), 1278-81 DOI: https://doi.org/10.1016/j.carres.2008.03.003

KathirvelAlagesan.; PrabhakarThennarasu.; Vinoth Kumar.; SadasivamSankarnarayanan.; ThayumanavanBalsamy.Identification of α-Glucosidase Inhibitors from Psidiumguajava leaves and Syzygiumcumini Linn.Seeds.International Journal of Pharma Sciences and Research (IJPSR). 2012, 3(2), 316-322

Singh, A. and Marar, T. Inhibitory effect of extracts of Syzygiumcumini and Psidiumguajava on glycosidases.Journal of Cell and Tissue Research. 2011, 11(1), 2535-2539

MenakshiBhat.; Smita S. Zinjarde.; Shobha Y. Bhargava.; Ameeta Ravi Kumar.; Bimba N. Joshi. Anti diabetic Indian Plants: A Good Source of Potent Amylase Inhibitors; Hindawi Publishing Corporation: Evidence-Based Complementary and Alternative Medicine 2008, 2011 6 pages DOI: https://doi.org/10.1093/ecam/nen040

Bopp, A.; De Bona, K.S.; Bellé, L.P.; Moresco, R.N.; Moretto, M.B. Syzygiumcumini inhibits adenosine deaminase activity and reduces glucose levels in hyperglycemic patients. Fundamental and clinical pharmacology. 2009, 23(4), 501-507.

Bellé L. P.; Bitencourt P. E. R.; Abdalla F.H.; de Bona K.S.; Alessandra Peres.; Maders L.D.K.; Moretto. Aqueous seed extract of Syzygiumcuminiinhibits the dipeptidyl peptidase IV and adenosine deaminase activities, but it does not change the CD26 expression in lymphocytes in vitro.Journal of Physiology and Biochemistry; 2012. 69(1), 119-124 DOI: https://doi.org/10.1007/s13105-012-0195-6

Modi, D.C.; Patel, J.K.; Shah, B.N.; Nayak, B.S. Anti-inflammatory activity of seeds of Syzygiumcuminilinn. An international journal of pharmaceutical sciences.2010, 1(1). 68-70

Kumar, R.; Ilavarasan, R.; Jayachandran, T.; Decaraman, M.; Mohan Kumar, R.; Aravindan, P.; Padmanabhan, N.;. Krishan, M. R. V. Anti-inflammatory activity of Syzygiumcumini seed African Journal of Biotechnology. 2008, 7(8), 941–943

Chaudhuri, A.K.; Siddhartha Pal.; Gomes, A.; Siddhartha Bhattacharya. Anti-inflammatory and related actions of Syzygiumcuminiseed extract. Physiotherapy research. 2006; 4(1), 5 – 14 DOI: https://doi.org/10.1002/ptr.2650040103

Singleton, V.L.; Rossi, J.A.; Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am. J. Enol. Vitic.1965, 16, pp 144-158

Sahreen, S.; Khan. M.R.; Khan, R.A. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chemistry, 2010, 122, 1205-1211

Brand Williams, W.; Cuvelier, M.E; Berset, C. Use of a free radical method to evaluate Antioxidant activity. Lebensmitted-WissenschaftundTechnologie / Food Science and Technology. 1995, 28, pp 25-30 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying on improved ABTS radical cationdecolorization assay. Free Radical Biology and Medicine.1999, 26, 1231-1237

Oyaizu, m. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition. 1986, 44, 307- 315 DOI: https://doi.org/10.5264/eiyogakuzashi.44.307

Umamaheswari, M.; Chatterjee, T.K. In vitro antioxidant activities of the fractions of Cocciniagrandis L. leAf extract. African J. Trad.Compl.Altern.Medicines. 2008, 5(1), 61-73 DOI: https://doi.org/10.4314/ajtcam.v5i1.31258

Casanova, E.; Garcia-Mina, J.M.; Clavo, M.I.; Antioxidant and antifungal activity of Verbena officinalis L. Leaves. Plant foods for human nutrition. 2008, 63(3), 93-145 DOI: https://doi.org/10.1007/s11130-008-0073-0

Kwek, S.Q.; Leong, L.P.; Bettens, R.; Effects of Different functional groups on Antioxidant activity. http://www.3.ntu.edu.sg.

Soares, J.R. Antioxidant activity of some extracts of Thymus zygis. Free Rad Ras. 1997 26, 469- 478 http://prr.hec.gov.pk/Chapters/226S-3.pdf DOI: https://doi.org/10.3109/10715769709084484

M.J.T.J.Arts., Assessing Antioxidant Activity, Mariken Arts, maastirchts, (2007), pg 23.

Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying on improved ABTS radical cationdecolorization assay. Free Radical Biology and Medicine.1999, 26, 1231-1237

Mitsuda, H.; Yasumoto, K.; Iwami, I. Antioxidative action of Indole compounds during the auto oxidation of Linoleic acid. Eiyotoshokuryo. 1966, 19, 210-214

Roginsky, V., Lissi, E, Review of methods to determine chain-breaking antioxidant activity in food. Food Chem, 2005, 92, 235-254. DOI: https://doi.org/10.1016/j.foodchem.2004.08.004

Omer Behan et al., Total phenolic compounds and antioxidant capacity of leAf, dry fruit and fresh fruit of feijoa (Accasellowiana, Myrtaceae), Journal of Medicinal Plants Research. 2010, 4(11), 1065-1072.

Gordan M.H.; The mechanism of antioxidant in vitro.Food Antioxidants. Elsevier: London/New York 1990. 1-18 DOI: https://doi.org/10.1007/978-94-009-0753-9_1

Sahreen, S.; Khan. M.R.; Khan, R.A. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chemistry, 2010, 122, 1205-1211 DOI: https://doi.org/10.1016/j.foodchem.2010.03.120

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Published

2015-05-31

How to Cite

Haroon, R., SeemalJelani, & Arshad, F. (2015). COMPARATIVE ANALYSIS OF ANTIOXIDANT PROFILES OF BARK, LEAVES AND SEEDS OF SYZYGIUM CUMINI (INDIAN BLACKBERRY). International Journal of Research -GRANTHAALAYAH, 3(5), 13–26. https://doi.org/10.29121/granthaalayah.v3.i5.2015.3010