IN VITRO ANTIBACTERIAL POTENTIAL OF DIFFERENT COMBINATION OF PLANT EXTRACT OF SOME COMMON WEED PLANTS AGAINST XANTHOMONAS ORYZAE AND AGROBACTERIUM SPP
DOI:
https://doi.org/10.29121/granthaalayah.v13.i12.2025.6607Keywords:
Polyherbal combination, Antibacterial Activity, Xanthomonas Oryzae, Agrobacterium Spp., Biopesticide, Phytochemicals, Sustainable Agriculture, Zone Of InhibitionAbstract [English]
The present study investigates the antibacterial activity of various polyherbal combinations against two significant plant pathogens, Xanthomonas oryzae (ATCC 35933) and Agrobacterium spp. (ATCC 12210), both of which are known to cause major crop losses. Using the agar well diffusion method, five different polyherbal combinations (F-1 to F-5) were evaluated at a concentration of 100 mg/ml. The results showed a progressive increase in antibacterial activity from F-1 to F-5, with F-5 exhibiting the largest zones of inhibition—21 mm against X. oryzae and 25 mm against Agrobacterium spp. This enhanced activity is attributed to the synergistic action of phytochemicals within the polyherbal mix. The findings highlight the potential of polyherbal combinations as eco-friendly biopesticides and suggest that such natural alternatives could play a significant role in sustainable agricultural practices. Further studies are recommended to validate these results under field conditions and optimize formulations for commercial use.
Downloads
References
Handa, S. S., Khanuja, S. P. S., Longo, G., and Rakesh, D. D. (2008). Extraction Technologies for Medicinal and Aromatic Plants (260). UNIDO and ICS-UNIDO.
Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Springer.
Heinrich, M., Barnes, J., Gibbons, S., and Williamson, E. M. (2009). Fundamentals of Pharmacognosy and Phytotherapy. Elsevier.
Isman, M. B. (2000). Plant Essential oils for Pest and Disease Management. Crop Protection, 19(8–10), 603–608. https://doi.org/10.1016/S0261-2194%2800%2900079-X DOI: https://doi.org/10.1016/S0261-2194(00)00079-X
Jain, D., Verma, A., and Pandey, R. (2015). Antibacterial Properties of Tridax Procumbens L. Against Human Pathogens. International Journal of PharmTech Research, 8(4), 229–233.
Kumar, A., and Singh, M. (2020). Phytochemical and Antimicrobial Screening of Calotropis Gigantea. International Journal of Herbal Medicine, 8(1), 44–49.
Mehta, V., Sharma, R., and Kapoor, S. (2017). Argemone Mexicana: A Review on its Traditional Uses and Pharmacological Activities. Asian Journal of Biomedical and Pharmaceutical Sciences, 7(63), 30–35.
Mishra, S., Chauhan, P. S., Chauhan, S., and Gaur, A. (2018). Antimicrobial Potential of Polyherbal Formulations. Journal of Plant Pathology, 100(1), 155–160.
Nisar, T., Iqbal, M., Raza, A., Safdar, M., and Iftikhar, F. (2017). Antimicrobial Activity of Herbal Extracts. Journal of Applied Pharmaceutical Science, 7(11), 193–198.
Okoli, R.I. et al. (2002). Phytotherapy Research, 16(8), 726-728. https://doi.org/10.1002/ptr.991 DOI: https://doi.org/10.1002/ptr.991
Pandey, R. R., Bajpai, P., and Singh, S. P. (2019). Efficacy of Herbal Extracts Against Agricultural Pathogens. Plant Protection Quarterly, 34(2), 65–72.
Rajan, P., Babu, S., and Meenakshi, S. (2019). Preliminary Screening of Antimicrobial Properties of Sida Acuta Leaf Extracts. World Journal of Pharmaceutical Research, 8(6), 245–252.
Rao, P., and Pillai, R. (2018). Evaluation of Antimicrobial Activity of Alternanthera Sessilis. Indian Journal of Pharmacognosy, 10(2), 77–83.
Sharma, A., Gupta, R., and Tiwari, S. (2019). Antimicrobial Potential of Datura Metel Extracts Against Clinical Isolates. Journal of Medicinal Plants Research, 13(4), 112–117.
Upadhyay, S., Dixit, A., and Bhattacharya, S. (2020). Phytochemical Synergy and Antimicrobial Effectiveness in Polyherbal Formulations. International Journal of Phytotherapy and Phytopharmacology, 4(2), 95–102.
Verma, S., and Rao, M. (2016). Antimicrobial Efficacy of Lantana Camara Against Human Pathogens. International Journal of Pharmacy and Pharmaceutical Sciences, 8(10), 99–104.
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Tanushri Saha, Suchi Modi

This work is licensed under a Creative Commons Attribution 4.0 International License.
With the licence CC-BY, authors retain the copyright, allowing anyone to download, reuse, re-print, modify, distribute, and/or copy their contribution. The work must be properly attributed to its author.
It is not necessary to ask for further permission from the author or journal board.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.





















