PRODUCTION OF ORGANIC ACID AND SOLUBILIZATION OF INORGANIC PHOSPHATE BY A BACTERIUM ISOLATED FROM CONTAMINATED SOIL

Authors

  • Sinal Tuscano Department Life Science, Ramnarain Ruia Autonomous College, L.N. Road, Matunga, Mumbai, India
  • Nilima Gajbhiye HOD, Department Life Science, Ramnarain Ruia Autonomous College, L.N. Road, Matunga, Mumbai, India https://orcid.org/0000-0002-3236-8622

DOI:

https://doi.org/10.29121/granthaalayah.v12.i1.2024.5470

Keywords:

Phosphate Solubilization, Organic Acid Production, Tricalcium Phosphate, Phosphate Solubilization Index

Abstract [English]

Many agricultural soils have significant phosphorus (P) reserves, much of which builds up because of frequent P fertilizer applications. However, roughly 95 to 99% of soil phosphorus is found as insoluble phosphates and is therefore unavailable for plant uptake. The current investigation characterized a bacterial strain that was obtained from contaminated soil and showed the ability to solubilize insoluble inorganic phosphates. An efficient phosphate-solubilizing bacterium was isolated in polluted soil in Mumbai. The phosphate solubilization index of this isolate was assessed using tribasic calcium phosphate-supplemented Pikovskaya’s (PVK) medium. After growing under constant agitation for seven days, the medium pH decreased from 7.0 to 3.5 units. Based on the colony morphology, microscopic analysis, and MALDI-TOF sequencing, the bacterial isolate was identified as Klebsiella pneumoniae. Phosphate solubilization was linked to a pH drop caused by bacterial growth in a medium with glucose as a carbon source. The secretion of organic acids by these phosphate-solubilizing bacteria is responsible for their ability to solubilize inorganic phosphate. GC-MS analysis revealed the presence of carbamic acid, dodecanoic acid, tetra decanoic acid, and trifluoroacetic acid in the culture supernatant. The amount of phosphate solubilized by the bacterium was determined by phosphomolybdate assay and was found to be 667.0 ug/ml which was much higher than the control bacterium S. aureus which was 131.0 ug/ml. To the best of our knowledge, this is the first report mentioning the isolation of phosphate solubilizing bacterium from polluted soil in Mumbai.

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References

Ben Farhat, M., Farhat, A., Bejar, W., Kammoun, R., Bouchaala, K., Fourati, A., Antoun, H., Bejar, S., & Chouayekh, H. (2009). Characterization of the Mineral Phosphate Solubilizing Activity of Serratia Marcescens CTM 50650 Isolated From the Phosphate Mine of Gafsa. Archives of Microbiology, 191(11), 815–824. https://doi.org/10.1007/s00203-009-0513-8

Bolan, N. S., Naidu, R., Mahimairaja, S., & Baskaran, S. (1994). Influence of Low-Molecular-Weight Organic Acids on the Solubilization of Phosphates. Biology and Fertility of Soils, 18(4), 311–319. https://doi.org/10.1007/BF00570634

Cao, Y., Fu, D., Liu, T., Guo, G., & Hu, Z. (2018). Phosphorus Solubilizing and Releasing Bacteria Screening from the Rhizosphere in a Natural Wetland. Water (Switzerland), 10(2), 1–15. https://doi.org/10.3390/w10020195

Chauhan, A., Guleria, S., Balgir, P. P., Walia, A., Mahajan, R., Mehta, P., & Shirkot, C. K. (2017). Tricalcium Phosphate Solubilization and Nitrogen Fixation by Newly Isolated Aneurinibacillus Aneurinilyticus CKMV1 from Rhizosphere of Valeriana Jatamansi and Its Growth Promotional Effect. Brazilian Journal of Microbiology, 48(2), 294–304. https://doi.org/10.1016/j.bjm.2016.12.001

De Campos, M., Antonangelo, J. A., & Alleoni, L. R. F. (2016). Phosphorus Sorption Index in Humid Tropical Soils. Soil and Tillage Research, 156, 110–118. https://doi.org/10.1016/j.still.2015.09.020

Haouas, A., el Modafar, C., Douira, A., Ibnsouda-Koraichi, S., Filali-Maltouf, A., Moukhli, A., & Amir, S. (2021). Alcaligenes Aquatilis GTE53: Phosphate Solubilising and Bioremediation Bacterium Isolated from New Biotope “Phosphate Sludge Enriched-Compost.” Saudi Journal of Biological Sciences, 28(1), 371–379. https://doi.org/10.1016/j.sjbs.2020.10.015

Henri, F., Laurette, N. G. O. N., Annette, D., & John, Q. (2008). Solubilization of Inorganic Phosphates and Plant Growth Promotion by Strains of Pseudomonas Fluorescens Isolated from Acidic Soils of Cameroon. African Journal of Microbiology Research, 2(7), 171–178. https://doi.org/10.1186/1471-2164-9-316

Janati, W., Mikou, K., El Ghadraoui, L., & Errachidi, F. (2022). Isolation and Characterization of Phosphate Solubilizing Bacteria Naturally Colonizing Legumes Rhizosphere in Morocco. Frontiers in Microbiology, 13(September). https://doi.org/10.3389/fmicb.2022.958300

Kadiri, D. D., Gorle, N., Varada, K., Peetala, R., & Peela, S. (2013). Isolation, Screening and Identification of Phosphate Solubilizing Bacteria From Different Regions of, 4(4), 518–526.

Karpagam, T., & Nagalakshmi, P. K. (2014). Isolation and Characterization of Phosphate Solubilizing Microbes from Agricultural Soil. Int.J.Curr.Microbiol. App.Sci, 3(3), 601–614.

Kaur, R., Kaur, R., Sharma, A., Kumar, V., Sharma, M., Bhardwaj, R., & Thukral, A. K. (2018). Microbial Production of Dicarboxylic Acids from Edible Plants and Milk Using GC-MS. Journal of Analytical Science and Technology, 9(1). https://doi.org/10.1186/s40543-018-0154-0

Kumar, A., Bhargava, P., & Rai, L. C. (2010). Isolation and Molecular Characterization of Phosphate Solubilizing Enterobacter and Exiguobacterium Species from Paddy Fields of Eastern Uttar Pradesh, India. African Journal of Microbiology Research, 4(9), 820–829.

Mahadevamurthy, M., Channappa, T., Sidappa, M., Raghupathi, M., & Nagaraj, A. (2016). Isolation of Phosphate Solubilizing Fungi from Rhizosphere Soil and Its Effect on Seed Growth Parameters of Different Crop Plants. Journal of Applied Biology and Biotechnology, 4(06), 022–026. https://doi.org/10.7324/jabb.2016.40604

Mardad, I., Serrano, A., & Soukri, A. (2013). Solubilization of Inorganic Phosphate and Production of Organic Acids by Bacteria Isolated from a Moroccan Mineral Phosphate Deposit. African Journal of Microbiology Research, 7(8), 626–635. https://doi.org/10.5897/AJMR12.1431

Noorjahan, A., Aiyamperumal, B., & Anantharaman, P. (2019). Isolation and Charecterisation of Seaweed Endophytic Fungi as an Efficient Phosphate Solubiizers. Biosciences, Biotechnology Research Asia, 16(1), 33–39. https://doi.org/10.13005/bbra/2718

Otani, T., Ae, N., & Tanaka, H. (1996). Phosphorus (P) Uptake Mechanisms of Crops Grown in Soils with Low P Status: II. Significance of Organic Acids in Root Exudates of Pigeonpea. Soil Science and Plant Nutrition, 42(3), 553–560. https://doi.org/10.1080/00380768.1996.10416324

Pande, A., Pandey, P., & Kaushik, S. (2017). Co-Inoculation of and Enhances Plant Growth of Maize () Under Green House and Field Condition. Korean Journal of Agricultural Science, 44(2), 196–210. https://doi.org/10.7744/kjoas.20170019

Pande, A., Pandey, P., Mehra, S., Singh, M., & Kaushik, S. (2017). Phenotypic and Genotypic Characterization of Phosphate Solubilizing Bacteria and Their Efficiency on the Growth of Maize. Journal of Genetic Engineering and Biotechnology, 15(2), 379–391. https://doi.org/10.1016/j.jgeb.2017.06.005

Rfaki, A., Nassiri, L., & Ibijbijen, J. (2015). Isolation and Characterization of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean (Vicia faba L.) in Meknes Region, Morocco. British Microbiology Research Journal, 6(5), 247–254. https://doi.org/10.9734/bmrj/2015/14379

Son, H. J., Park, G. T., Cha, M. S., & Heo, M. S. (2006). Solubilization of Insoluble Inorganic Phosphates by a Novel Salt- and pH-Tolerant Pantoea Agglomerans R-42 Isolated from Soybean Rhizosphere. Bioresource Technology, 97(2), 204–210. https://doi.org/10.1016/j.biortech.2005.02.021

Susilowati, L. E., Kusumo, B. H., & Arifin, Z. (2019). Screening of the Drought Tolerant Phosphate Solubilizing Bacteria in Dissolving P-Inorganic. Journal of Physics: Conference Series, 1402(5). https://doi.org/10.1088/1742-6596/1402/5/055082

Umeh, S. I., & Sapele, A. (2015). Effect of Phosphate Solubilizing Bacteria on. Nigerian Journal of Microbiology, 29(August), 3159–3166.

Vazquez, P., Holguin, G., Puente, M. E., Lopez-Cortes, A., & Bashan, Y. (2000). Phosphate-Solubilizing Microorganisms Associated with the Rhizosphere of Mangroves in a Semiarid Coastal Lagoon. Biology and Fertility of Soils, 30(5–6), 460–468. https://doi.org/10.1007/s003740050024

Wang, Z., Xu, G., Ma, P., Lin, Y., Yang, X., & Cao, C. (2017). Isolation and Characterization of a Phosphorus-Solubilizing Bacterium from Rhizosphere Soils and Its Colonization of Chinese Cabbage (Brassica Campestris ssp. Chinensis). Frontiers in Microbiology, 8(JUL), 1–12. https://doi.org/10.3389/fmicb.2017.01270

Wei, Y., Zhao, Y., Shi, M., Cao, Z., Lu, Q., Yang, T., Fan, Y., & Wei, Z. (2018). Effect of Organic Acids Production and Bacterial Community on the Possible Mechanism of Phosphorus Solubilization During Composting with Enriched Phosphate-Solubilizing Bacteria Inoculation. Bioresource Technology, 247, 190–199. https://doi.org/10.1016/j.biortech.2017.09.092

Zheng, B. X., Ibrahim, M., Zhang, D. P., Bi, Q. F., Li, H. Z., Zhou, G. W., Ding, K., Peñuelas, J., Zhu, Y. G., & Yang, X. R. (2018). Identification and Characterization of Inorganic-Phosphate-Solubilizing Bacteria from Agricultural Fields With a Rapid Isolation Method. AMB Express, 8(1). https://doi.org/10.1186/s13568-018-0575-6

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Published

2024-01-31

How to Cite

Tuscano, S. ., & Gajbhiye, N. (2024). PRODUCTION OF ORGANIC ACID AND SOLUBILIZATION OF INORGANIC PHOSPHATE BY A BACTERIUM ISOLATED FROM CONTAMINATED SOIL. International Journal of Research -GRANTHAALAYAH, 12(1), 14–25. https://doi.org/10.29121/granthaalayah.v12.i1.2024.5470