VALIDATION OF MORPHOLOGICAL CRITERIA FOR CLONAL IDENTIFICATION IN BUDWOOD GARDENS OF RUBBER TREE (HEVEA BRASILIENSIS MUELL. ARG.) CLONES USING MICROSATELLITE MARKERS

H. Michel Kouakou; A.A. Eliathe Elabo; I. Jésus Fofana; N. Désiré Pokou; A. Simon-Pierre N’guetta; Y. Michel Gnagne; A. Freddy Yapi; Yao S.D. Martial; Nafan Diarrassouba; Samuel Obouayeba

doi:10.29121/granthaalayah.v10.i10.2022.4826

Authors

Kouakou H. Michel Doctor, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory coast and PhD, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory coast and Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Elabo A.A. Eliathe Doctor, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory coast
Fofana I. Jésus Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Pokou N. Désiré Doctor, Central Laboratory of Biotechnology, National Center for Agronomic Research (CNRA), 01 P.O. Box 1740 Abidjan 01, Ivory Coast
N’guetta A. Simon-Pierre Professor, Laboratory of Genetics, University Félix Houphouët Boigny, 22 P.O. Box 582, Abidjan 22, Ivory Coast
Gnagne Y. Michel Doctor, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory coast
Yapi A. Freddy Doctor, Faculty of Biological Sciences, Department of Biology and Plant Physiology, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Yao S.D. Martial Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Diarrassouba Nafan Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Obouayeba Samuel Doctor, Agronomy physiology Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory coast

DOI:

https://doi.org/10.29121/granthaalayah.v10.i10.2022.4826

Keywords:

Hevea Brasiliensis, Rubber Tree Clones, Budwood Garden, Microsatellite Marker, Morphological Criteria, Error

Abstract [English]

The inability of almost all rubber tree nursery growers to distinguish between the different rubber tree clones in a budwood garden (JBG) often leads to clonal mixtures. This makes the application of latex harvesting technology unsuitable, which negatively impacts the yield and / or the economic life of the plantations. To overcome this problem, a clonal identification method based on the use of seven (7) morphological criteria in JBG (budwood garden) was developed. Knowing that the morphological criteria are influenced by the environment, the visual recognition of the plants seems approximate or inefficient. In order to check the effectiveness of the morphological criteria, five (5) rubber tree genotypes from two JBGs (budwood gardens) were characterized both morphologically and by microsatellite markers. Characterization by microsatellites was carried out on twelve (12) loci and involved a sample of 25 individuals per genotype. This first study on the use of microsatellite markers revealed that three (3) microsatellite markers (Hb36, Hb43 and Hb110) best discriminate the genotypes (GT1, PB217, IRCA41, IRCA230 and IRCA331) cultivated in Côte d'Ivoire. It also showed that an individual declared non-compliant by the morphological method has 89% chance of being genetically non-compliant, one could be mistaken by 11% in declaring an individual non-compliant while it is genetically compliant (type I error). In addition, an individual declared compliant by the morphological method has a 76% chance of being genetically non-compliant, one could also be mistaken by 24% in declaring compliant while it is genetically non-compliant (type II error).

Downloads

Download data is not yet available.

References

Assiri, A. A., Konan, A., N’Guessan, K.F., Kébé, B.I., Kassin, K.E., Couloud, J.Y., Yapo, A. R., Yoro, G.R., and Yao, K.A. (2015). Comparaison de deux techniques de replantation cacaoyère sur antécédents culturaux non-forestiers en Côte d’Ivoire. African Crop Science Journal, 23(4), 365-378. http://dx.doi.org/10.4314/acsj.v23i4.6.

Bayes, T. (1763). An Essay Towards Solving a Problem in the Doctrine of Chances, Philosophical Transactions of The Royal Society of London 53, 370-418. https://doi.org/10.1098/rstl.1763.0053.

Bayes, T. (1958). Studies in the History of Probability and Statistics: IX. Thomas Bayes’s Essay Towards Solving a Problem in the Doctrine of Chances. (Bayes’s Essay in Modernized Notation), Biometrika 45, 296-315. https://doi.org/10.2307/2333180.

Burow, G., Franks, C. D., Xin, Z., Burke, J. J. (2012). Genetic Diversity in a Collection of Chinese Sorghum Landraces Assessed By Microsatellites. American Journal of Plant Sciences 3, 1722-1729. http://dx.doi.org/10.4236/ajps.2012.312210.

Chaabane, R., Babay, E., Sahari, K., Khamassi, K., Ben, S. H., Gharbi, M. S., El, F. M., Deghaies, M., Ben, N. M. (2007). Choix des amorces microsatellites pour l’analyse de la diversité génétique du blé tendre : Acte des 14èmes journées scientifiques sur les résultats de la recherche Agricoles, Communication. http://dx.doi.org/10.13140/2.1.1539.6166.

Clément, D. A., Legnate, H., Seguin, M., Carron, M. P., Leguen, V., Chapuuset, T., and Schultes, R. E. (1990). A Briet Taxonomic View of The Genus Hevea. Malaysian Rubber Research and Development Board Monograph N° 14, Kuala Lumpur.

Compagnon, P. (1986). Le Caoutchouc Naturel : Biologie, Culture, Production. Maisonnneuve Et Larose.

Ghebru, B., Schmidt, R. J., Bennetzen, J. L. (2002). Genetic Diversity of Eritrean Sorghum Landraces Assessed with Simple Sequence Repeat (SSR) Markers. Theoretical and Applied Genetics 105, 229-236. http://dx.doi.org/10.1007/s00122-002-0929-x.

Giovani, S.F.B. (2005). Estimation and Inference in Dynamic Unbalanced Panel-Data Models With A Small Number of Individuals. Stata Journal 5(4), 473-500. https://doi.org/10.1177/1536867X0500500401.

Gnagne, Y.M., Elabo, A.A.E., Okoma, K.K., Konan, E., and Zakra, N. (2014a). Clef d’identification en Jardin à bois de greffes des clones de Hevea brasiliensis vulgarisés en Côte d’Ivoire, Poster.

Keli, J.Z., Obouayeba, S., Zehi, B. (1992). Influence de quelques systèmes vivriers sur le comportement des jeunes hévéas en basse Cote d’Ivoire. Systèmes Agricoles en Afrique 2(1), 41-48.

Kouakou H. M. (2017). Détermination du niveau de fiabilité de la méthode d’identification morphologique en jardin à bois des clones d’Hevea brasiliensis (Euphorbiaceae) diffusés en Côte d’Ivoire par utilisation des marqueurs microsatellites. Mémoire de Master de génétique et amélioration des espèces végétales de l’Université Félix Houphouët Boigny-Abidjan, Côte d’ivoire.

Le Guen, V. F., Doaré, C., Weber, And Seguin, M. (2009). Genetic Structure of Amazonian Populations of Hevea Brasiliensis Is Shaped by Hydrographical Network and Isolation By Distance. Tree Genetics and Genomes 5, 673-683. https://doi.org/10.1007/s11295-009-0218-9.

Obouayeba, S. (2005). Contribution à la détermination de la maturité physiologique de l’écorce pour la mise en saignée d'Hevea brasiliensis Muell. Arg. (euphorbiacée) : normes d'ouverture. Thèse de doctorat Unique de l’Université de Cocody, Côte d’Ivoire.

Peakall, R. and Smouse, P. E. (2012). Genalex 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research an Update. Bioinformatics 28(19), 2537-2539.

Penot, M. E. (2001). Stratégies paysannes et évolution des savoirs : l'hévéaculture agroforestière indonésienne. Thèse de Doctorat de l’Université Montpellier I.

Perrier, X. and Jacquemoud-Collet, J. (2006). DARwin software. Version 6.0, Montpellier.

Pritchard, J.K., Stephens, M. and Donnelly, P., (2000). Inference of Population Structure Using Multilocus Genotype Data. Genetics 155, 945-959. https://doi.org/10.1093/genetics/155.2.945.

Yapi, A. F. (2013). Identification morphologique en Jardin à bois de greffes de clones de Hevea brasiliensis Muell. AGR. (Euphorbiaceae) diffusés en Côte d’Ivoire. Mémoire de master de l’Université de Cocody-Abidjan, Côte d’ivoire.