SEASONAL VARIATION AND DIVERSITY OF BACTERIA OF THE VIBRIO AND SALMONELLA GENERA ISOLATED IN A FEW UNDERGROUND WATER POINTS DEVELOPED IN THE COMMUNE OF NTUI (MBAM-ET-KIM DEPARTMENT, CENTER-CAMEROON)

Samuel Davy Baleng; Olive Vivien Noah Ewoti; Berenger Patrick Tchinda Kenne; Claire Stéphane Metsopkeng; Morelle Raisa Tagne Djiala; Pélagie Ladibé; Moses Nola

doi:10.29121/granthaalayah.v10.i10.2022.4815

Authors

Samuel Davy Baleng Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon
Olive Vivien Noah Ewoti Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon
Berenger Patrick Tchinda Kenne Laboratory of Microbiology, Department of Microbiology, Faculty of Sciences, University of Yaounde, Cameroon
Claire Stéphane Metsopkeng Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon
Morelle Raisa Tagne Djiala Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon
Pélagie Ladibé Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon
Moses Nola Laboratory of Hydobiology and Environment, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde 1, Cameroon

DOI:

https://doi.org/10.29121/granthaalayah.v10.i10.2022.4815

Keywords:

BHAM, Pathogenic Bacteria, Groundwater, Abiotic Variables, Seasons

Abstract [English]

A study aimed at evaluating the seasonal variation of Vibrio and Salmonella in the developed groundwater used for drinking water in the commune of Ntui, Center Region of Cameroon was conducted from January to July 2021. The microorganisms sought were Aerobic Heterotrophic Mesophilic Bacteria (BHAM), bacteria of the Vibrio and Salmonella genera. These bacteria were isolated from ten (10) groundwater points by the surface spreading technique on ordinary agar for BHAMs and that of filter membranes on Thiosulfate Citrate Bile Sucrose (TCBS) and Salmonella-Shigella (SS) media. for Vibrio and Salmonella respectively. Some abiotic parameters such as temperature, pH, among others were evaluated using the usual techniques.
The results show that some physico-chemical parameters such as temperature vary relatively between the sampling points. There is a slightly acidic average pH of 6.86 ± 0.57 U.C which is therefore a function of the lateritic soils crossed by the waters. Bacteriological analyzes revealed that these waters have a high and varied bacterial load. Densities of pathogenic bacteria reached an average of 80 CFU/100mL for Vibrio and 7.4.102 CFU/100mL for Salmonella. The most represented bacterial species identified were Vibrio cholerae and Salmonella enterica. These germs can be the cause of cholera and typhoid epidemics in this municipality. Significant correlations (P<0.05) between bacterial abundances and pH were observed. The degradation of the quality of these waters is caused by their proximity to sources of pollution. According to European Union standards, these waters are not recommended for human consumption without any prior treatment.

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