INSIGHT INTO EXTRACELLULAR POLYMERIC SUBSTANCES EPS EXTRACTION AND QUANTIFICATION TECHNIQUES: CAUSES, MECHANISMS, AND APPLICATIONS MINI REVIEW
DOI:
https://doi.org/10.29121/granthaalayah.v10.i3.2022.4518Keywords:
Biofilm, Extracellular Polymeric Substances (EPS), Microorganisms, LysisAbstract [English]
Microorganisms have a secret world, which is full of baffling phenomena. Without studying this world, we cannot understand the concept of biofilm formation, which is based on the aggregation of different kinds of microorganisms to create micro-colonies. The mysterious world of biofilm is fairly understood. Due to the sophisticated complexity of its structure and architecture, biofilm has many essential properties which had made it an important study field for many researchers in different aspects; public health, water treatment, and especially microbiology. By going into the complexity of biofilm structure, studying Extracellular Polymeric Substances (EPS) for the last decade has provided researchers with great evidence regarding biofilm formation. EPS extraction is the first step toward being able to study the composition and properties of various microbial communities. This review covers EPS extraction techniques and their significant in life.
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Beyenal, Haluk, Conrad Donovan, Zbigniew Lewandowski and Gary Harkin (2004). Three-dimensional biofilm structure quantification. Journal of Microbiological Methods 59 : 395-413. doi : Retrieved from https://doi.org/10.1016/j.mimet.2004.08.003 DOI: https://doi.org/10.1016/j.mimet.2004.08.003
Comte, S., G. Guibaud and M. Baudu (2006). Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties : Part I. Comparison of the efficiency of eight EPS extraction methods. Enzyme and Microbial Technology 38 : 237-245. doi : Retrieved from https://doi.org/10.1016/j.enzmictec.2005.06.016 DOI: https://doi.org/10.1016/j.enzmictec.2005.06.016
Donlan, Rodney M. (2002). Biofilms : microbial life on surface. Emerging infectious diseases 8 : 881-890. doi : 10.3201/eid0809.020063 Retrieved from https://doi.org/10.3201/eid0809.020063 DOI: https://doi.org/10.3201/eid0809.020063
Flemming, Hans-Curt, Thomas R. Neu and Daniel J. Wozniak (2007). The EPS matrix : the "house of biofilm cells". Journal of bacteriology 189 : 7945-7947. doi : 10.1128/JB.00858-07 Retrieved from https://doi.org/10.1128/JB.00858-07 DOI: https://doi.org/10.1128/JB.00858-07
Lewandowski, Zbigniew and Haluk Beyenal (2019). Fundamentals of biofilm research : CRC pres
Liu, Hong and Herbert H. P. Fang (2002). Extraction of extracellular polymeric substances (EPS) of sludges. Journal of Biotechnology 95 : 249-256. doi : Retrieved from https://doi.org/10.1016/S0168-1656(02)00025-1 DOI: https://doi.org/10.1016/S0168-1656(02)00025-1
Nielsen, PH and A Jahn (1999). Extraction of EPS. In "Microbial Extracellular Polymeric Substances : Characterization, Structure and Function”. (Eds J. Wingender, TR Neu and HC Flemming.) pp. 50-72. In Extraction of EPS. In "Microbial Extracellular Polymeric Substances : Characterization, Structure and Function”. (Eds J. Wingender, TR Neu and HC Flemming.) pp. 50-72 : Springer-Verlag : Berlin.
Reipa, Vytas, Jamie Almeida and Kenneth D. Cole (2006). Long-term monitoring of biofilm growth and disinfection using a quartz crystal microbalance and reflectance measurements. Journal of Microbiological Methods 66 : 449-459. doi : Retrieved from https://doi.org/10.1016/j.mimet.2006.01.016 DOI: https://doi.org/10.1016/j.mimet.2006.01.016
Sheng, Guo-Ping, Han-Qing Yu and Xiao-Yan Li (2010). Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems : A review. Biotechnology Advances 28 : 882-894. doi : Retrieved from https://doi.org/10.1016/j.biotechadv.2010.08.001 DOI: https://doi.org/10.1016/j.biotechadv.2010.08.001
Sheng, Guo-Ping, Han-Qing Yu and Zhou Yu (2005). Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila. Applied Microbiology and Biotechnology 67 : 125-130. doi : 10.1007/s00253- 004-1704-5 Retrieved from https://doi.org/10.1007/s00253-004-1704-5 DOI: https://doi.org/10.1007/s00253-004-1704-5
Spaeth, ROSEMARIE and STEFAN Wuertz (2000). Extraction and quantification of extracellular polymeric substances from wastewater. Lancaster, Pa : Technomic Publishers, Retrieved from https://books.google.co.in/books?hl=en&lr=&id=X0xZDwAAQBAJ&oi=fnd&pg=PA51&dq=Extraction+and+quantification+of+extracellular+polymeric+substances+from+wastewater&ots=EJsNZqrj7G&sig=ESTI_YK6bA3n9JSOfK3y25zDYFo&redir_esc=y#v=onepage&q=Extraction%20and%20quantification%20of%20extracellular%20polymeric%20substances%20from%20wastewater&f=false
Wagner, Michael, Natalia P. Ivleva, Christoph Haisch, Reinhard Niessner and Harald Horn (2009). Combined use of confocal laser scanning microscopy (CLSM) and Raman microscopy (RM) : Investigations on EPS - Matrix. Water Research 43 : 63-76. doi : Retrieved from https://doi.org/10.1016/j.watres.2008.10.034 DOI: https://doi.org/10.1016/j.watres.2008.10.034
Yang, Xinmin, Haluk Beyenal, Gary Harkin and Zbigniew Lewandowski (2000). Quantifying biofilm structure using image analysis. Journal of Microbiological Methods 39 : 109-119. doi : Retrieved from https://doi.org/10.1016/S0167-7012(99)00097-4 DOI: https://doi.org/10.1016/S0167-7012(99)00097-4
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