IDENTIFICATION OF THE DISCRIMINATING MOLECULAR PROFILES OF FIVE MAIN RUBBER TREE (HEVEA BRASILIENSIS) GENOTYPES RECOMMENDED IN IVORY COAST

H. Michel Kouakou; A. E. Elabo Angeline; Inza Jésus Fofana; N’da Désiré Pokou; Kouakou Fulgence Brou; Nafan Diarrassouba; Saraka Didier Martial Yao; Arnaud Freddy Yapi; Yedoh Michel Gnagne; Samuel Obouayeba

doi:10.29121/ijoest.v7.i2.2023.463

Authors

Kouakou H. Michel Doctor, Ex-engineer, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory Coast, Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast, Doctor, Central Laboratory of Biotechnology, National Center for Agronomic Research (CNRA), 01 P.O. Box 1740 Abidjan 01, Ivory Coast
Angeline. A. E. Elabo Doctor, Ex-engineer, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory Coast
Inza Jésus Fofana Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
N’da Désiré Pokou Doctor, Central Laboratory of Biotechnology, National Center for Agronomic Research (CNRA), 01 P.O. Box 1740 Abidjan 01, Ivory Coast
Kouakou Fulgence Brou Doctor, Ex-engineer, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory Coast
Nafan Diarrassouba Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Saraka Didier Martial Yao Doctor, Faculty of Biological Sciences, Department of Biochemistry-Genetics, Pedagogical and Research Unit of Genetics, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Arnaud Freddy Yapi Doctor, Faculty of Biological Sciences, Department of Biology and Plant Physiology, University Péléforo Gon Coulibaly (UPGC), P.O. Box 1328 Korhogo, Ivory Coast
Yedoh Michel Gnagne Doctor, Ex-engineer, Genetic Improvement Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory Coast
Samuel Obouayeba Doctor, Agronomy Physiology Operation, National Center for Agronomic Research (CNRA), Research Station of Bimbresso, 01 P.O. Box 1536 Abidjan 01, Ivory Coast

DOI:

https://doi.org/10.29121/ijoest.v7.i2.2023.463

Keywords:

Hevea Brasiliensis, Discriminating Microsatellite Sequences, Ivory Coast

Abstract

This study was undertaken to identify the microsatellite markers characteristic of rubber tree genotypes, producers of natural rubber. Twelve pairs of microsatellite primers obtained from expressed sequence tags (EST), available in the GENBANK genomic bank, were used to screen a sample of 40 individuals, that is, 8 per genotype. These microsatellite loci are di, tri or tetranucleotides, patterned perfect, imperfect, compound or complex. This study on the GT1, PB217, IRCA41, IRCA230 and IRCA331 genotypes, recommended in Ivory Coast, showed that it is possible to characterize them by using three microsatellite loci, such as Hb36, Hb43 and Hb110. The abundance of repeats observed in the genome of rubber tree clones was similar to those reported in other works. These can be exploited and used in rubber tree clonal improvement programs.

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