FACILE IN VITRO GLASS SLIDE LIGHT MICROSCOPY METHOD USING TETRACYCLINE TO VISUALIZE A REPETITIVE PATTERN IN AERIAL PLANT ROOT TIPS FILAMENTOUS NETWORK

Authors

DOI:

https://doi.org/10.29121/granthaalayah.v8.i12.2020.2699

Keywords:

Tetracycline, Adhesion Cellulose, Orchid Air Roots, Plant Cell Apoptosis, Plant Cell Wall, Tetracycline Cell Damage, Roots Filaments Repetitive Pattern

Abstract

The main purpose of this manuscript is to introduce a facile light microscopy methodology to visualize plant roots filaments. In a previous manuscript in vitro experiments on freshly plucked human hair roots documented the commonly used antibiotic Tetracycline (TE) deleterious effect on soft tissue, severe enough to allow for visualization of an underlying filamentous skeleton. In this manuscript, TE was also evaluated in a similar fashion of in vitro experiments, this time aerial plant roots were immersed in liquid Tetracycline. Images and video recordings are presented where plant aerial root tissue cells appeared to interact with Tetracycline, thus allowing for exposure of an underlying filamentous network. These filaments were documented undergoing biosorption of Tetracycline, thus indicating a probable keratin base. It is emphasized that a literature search showed similar, albeit visually different displays of roots filaments obtained by using a Scanning Electron Microscopy. The method herein introduced could be an adjunct to existing established methodology in root function research. Two salient advantages are identified, firstly that the essential minimal material and equipment is limited to a light microscope, glass slides, chosen biological material, water and powder Tetracycline. Secondly, the speed in obtaining results would offer researchers a preliminary or perhaps a final correct conclusion.

Downloads

Download data is not yet available.

References

Embi AA. (2020) THE HUMAN HAIR FOLLICLE AS SENTINEL FOR DRUGS EVALUATION: DEMONSTRATION OF TETRACYCLINE ADHESION TO HAIR FOLLICLE AS PROPOSED MECHANISM IN DYSFUNCTIONAL HAIR LOSS. IJRG 8(11), 324-332. https://doi.org/10.29121/granthaalayah.v8.i11.2020.2521 DOI: https://doi.org/10.29121/granthaalayah.v8.i11.2020.2521

Scherlag BJ, Sahoo K, Embi AA. Novel and Simplified Method for Imaging the Electromagnetic Energy in Plant and Animal Tissue. Journal of Nanoscience and Nanoengineering. 2016 Vol 2 No 1, pp 6-9

Qiu, Y., Antony, E., Doganay, S., Koh, H. R., Lohman, T. M., & Myong, S. (2013). Srs2 prevents Rad51 filament formation by repetitive motion on DNA. Nature communications, 4, 2281. https://doi.org/10.1038/ncomms3281 DOI: https://doi.org/10.1038/ncomms3281

Kim, Y.W., Moon, H.K. & Son, S.C. Repetitive somatic embryogenesis and plant regeneration in Zizyphus jujuba Mill. In Vitro Cell.Dev.Biol.-Plant 42, 247–251 (2006). https://doi.org/10.1079/IVP2006754 DOI: https://doi.org/10.1079/IVP2006754

Vaughan S, Kohl L, Ngai I, Wheeler RJ, Gull K. A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei. Protist. 2008 Jan;159(1):127-36. doi: 10.1016/j.protis.2007.08.005. Epub 2007 Oct 22. PMID: 17945531. DOI: https://doi.org/10.1016/j.protis.2007.08.005

Margas M, Piotrowicz-Cieślak AI, Michalczyk DJ, Głowacka K. A Strong Impact of Soil Tetracycline on Physiology and Biochemistry of Pea Seedlings. Scientifica (Cairo). 2019 Jan 10; 2019:3164706. doi: 10.1155/2019/3164706. PMID: 30733888; PMCID: PMC6348853. DOI: https://doi.org/10.1155/2019/3164706

David Attenborough (1995:166) The Private Life of Plants. BBC Books

Zotz G, Winkler U (2013) Aerial roots of epiphytic orchids: the velamen radicum and its role in water and nutrient uptake. Oecologia, 171(3): 733–741 DOI: https://doi.org/10.1007/s00442-012-2575-6

Manali Rathod, Soumya Haldar, Shaik Basha. (2015) Nanocrystalline cellulose for removal of tetracycline hydrochloride from water via biosorption: Equilibrium, kinetic and thermodynamic studies Ecological Engineering 84:240-249

DOI: 10.1016/j.ecoleng.2015.09.031 DOI: https://doi.org/10.1016/j.ecoleng.2015.09.031

Min, G., Yang, C., Tong, X., & Zhai, Z. (2008). Assembly characteristics of plant keratin intermediate filaments in vitro. Science in China Series C: Life Sciences, 42, 485-493.

DOI:10.1007/BF02881772 DOI: https://doi.org/10.1007/BF02881772

Corpus ID: 13003144.

Downloads

Published

2021-01-02

How to Cite

Embi, A. A. (2021). FACILE IN VITRO GLASS SLIDE LIGHT MICROSCOPY METHOD USING TETRACYCLINE TO VISUALIZE A REPETITIVE PATTERN IN AERIAL PLANT ROOT TIPS FILAMENTOUS NETWORK. International Journal of Research -GRANTHAALAYAH, 8(12), 155–166. https://doi.org/10.29121/granthaalayah.v8.i12.2020.2699

Most read articles by the same author(s)

1 2 > >>