PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTI-CYTOTOXIC AND ANTICANCER EFFECTS OF GALINSOGA PARVIFLORA AND VERNONIA POLYANTHES (ASTERACEAE) EXTRACTS

  • Sula M. V. Feleti Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espirito Santo, Vitória, Espírito Santo – Brazil
  • Renê L. Aleluia Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espirito Santo, Vitória, Espírito Santo – Brazil
  • Suiany V. Gervásio Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espirito Santo, Vitória, Espírito Santo – Brazil
  • Jean Carlos V. Dutra Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espirito Santo, Vitória, Espírito Santo – Brazil https://orcid.org/0000-0003-3893-9470
  • Jessica R. P. Oliveira Programa de Pós Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde - CCS, Universidade Federal do Espirito Santo - UFES, Marechal Campos Av., 1468, Vitoria 29043-900, ES, Brazil
  • Rita de Cássia R. Gonçalves Programa de Pós Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde - CCS, Universidade Federal do Espirito Santo - UFES, Marechal Campos Av., 1468, Vitoria 29043-900, ES, Brazil
  • Claudia M. Jamal Laboratório de Química de Produtos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil
  • Ricardo M. Kuster Laboratório de Cromatografia, Departamento de Química, Universidade Federal do Espírito Santo, Vitória, Espírito Santo – Brazil
  • Beatriz G. Brasileiro Instituto Federal do Sudeste de Minas Gerais, Campus Muriaé – Minas Gerais – Brazil
  • Maria do Carmo P. Batitucci Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espirito Santo, Vitória, Espírito Santo – Brazil
Keywords: Phenolic Content, Mass Spectrometry, Antioxidant Activity, MTT Assay

Abstract

The study was designed to investigate the chemical composition and the biological effects of G. parviflora and V. polyanthes ethanolic extracts in vitro. Total content of phenols, flavonoids and tannins was quantified by spectrophotometry; chemical characterization was permed by mass spectrometry (ESI (-) FT-ICR MS and APCI (+) FT-ICR MS analysis). Antioxidant activities were determined by FRAP and Fe2+ chelating methods. Extracts cytotoxicity was evaluated in human lymphocytes, sarcoma-180 (S-180) and human gastric adenocarcinoma (AGS) cells, by MTT assay. V. polyanthes presented higher total content of tannins and G. parviflora presented higher amount of phenols and flavonoids. Chemical characterization showed the presence of flavonoids, phenolic acids and sesquiterpene lactones in V. polyanthes extract, and steroids, phenolic acids and fatty acids (Poly Unsaturated Fatty Acids - PUFA) in G. parviflora extract. V. polyanthes extract stood out in the Fe2+ chelation test. G. parviflora extract did not present outstanding antioxidant results in the tested protocols. Both species showed a tendency to promote cytotoxicity in human lymphocyte cells. Regarding the antiproliferative effect, both species were able to reduce S-180 cell viability and G. parviflora extract showed high antiproliferative potential in the assay with AGS cells. These findings reinforce the medicinal use of these plants, as well as suggest their potential use for the development of new drugs and for the treatment of cancers.

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Published
2020-10-26
How to Cite
Feleti, S. M. V., Aleluia, R. L., Gervásio, S. V., Dutra, J. C. V., R. P. Oliveira, J., Rita de Cássia R. Gonçalves, R. de, Jamal, C. M., Kuster, R. M., Brasileiro, B. G., & Batitucci, M. do C. P. (2020). PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTI-CYTOTOXIC AND ANTICANCER EFFECTS OF GALINSOGA PARVIFLORA AND VERNONIA POLYANTHES (ASTERACEAE) EXTRACTS. International Journal of Research -GRANTHAALAYAH, 8(10), 84-98. https://doi.org/10.29121/granthaalayah.v8.i10.2020.1782