• Roshnie Singh Faculty Of Natural Sciences, University Of Guyana, Berbice Campus, Tain, Guyana https://orcid.org/0000-0001-8759-729X
  • Dharamdeo Singh Faculty Of Agriculture Science, University Of Guyana, Turkeyen Campus, Georgetown, Guyana
  • Phillip Da Silva Faculty Of Natural Sciences, University Of Guyana, Berbice Campus, Tain, Guyana
  • Gomathinayagam Subramanian Faculty Of Agriculture Science, University Of Guyana, Berbice Campus, Tain, Guyana
Keywords: Escherichia Coli, Aerobic Bacteria, Raw Cow’s Milk, Contamination, Environment, Predisposed


Milk is one of the main sources of nutrients to animals, but due to its high water activity, it serves as an ideal medium for the growth of microorganisms. The purpose of this research was to evaluate the microbial load in raw cow’s milk and to identify the possible sources of raw milk contamination in Joanna. Fifteen (15) raw milk samples were collected randomly according to the simple random sampling method and farmers were interviewed. Raw milk samples were analysed for Escherichia coli and aerobic bacteria. From the 15 samples of raw milk, contamination with E. coli (93.33%) and aerobic bacteria (100%) was observed. Also, several factors, milk handling practices, unhygienic practices, extensive farming systems, and lack of general zoonoses exposure practices and awareness, predisposed raw milk to microbial contamination. This research concluded that the quality of milk was unsatisfactory since it was heavily contaminated with bacteria. It is recommended that there should be an increase in the agriculture extension education and awareness programme to help improve milk quality.


Lanou, Amy Joy, Susan E. Berkow, and Neal D. Barnard. "Calcium, dairy products, and bone health in children and young adults: a reevaluation of the evidence." Pediatrics 115.3 (2005): 736-743. DOI: https://doi.org/10.1542/peds.2004-0548

Chambers, J. V. "Dairy Microbiology Handbook." New York (2002): 39-90.

Omore, A., Lore, T., Staal, S., Kutwa, J., Ouma, R., Arimi, S., and Kang’ethe, E. Addressing the public health and quality concerns towards marketed milk in Kenya. Smallholder Dairy Project Research and Development, Report 3. (2005).

Rodojcic-Prodaova, D., and T. Necev. "Most common agents of subclinical mastitis in cows on private and communal farms in the Republic of Macedonia." Vet. Glasnik 45 (1991): 745-747.

Eberhart, R.J. Coliform Mastitis. J. Am. Vet. Assoc., 170 (1977): 1160-1163.

Parekh, T. S., and R. Subhash. "Molecular and bacteriological examination of milk from different milch animals with special reference to coliforms." Current Research in Bacteriology 1.2 (2008): 56-63. DOI: https://doi.org/10.3923/crb.2008.56.63

World Health Organization. Safer Food for Better Health. A Global WHO food strategy. pp. 2, (2009). Retrieved from: http://www.who.int/mip2001/files/2297/FoodsafetyStrategy.doc.

Food hygiene. (2010). Retrieved from: https://web.archive.org/web/20151211131645/https://www.bupa.com.au/health-and-wellness/health-information/healthy-living/nutrition/eating/doc/food-hygiene.

Shirima, G.M., Fitzpatrick, J., Cleaveland, S., Kambarage, D.M., Kazwala, R.R., Kunda, J. and Fren1ch, N.P. Participatory survey on zoonotic diseases affecting livestock keeping communities in Tanzania. Journal of Animal and Veterinary Advances 2.4 (2003): 253 - 258.

Sivapalasingam, S., Friedman, C. R., Cohen, L., &Tauxe, R. V. Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. Journal of food protection, 67.10 (2004): 2342-2353. DOI: https://doi.org/10.4315/0362-028X-67.10.2342

Al-Tahiri, R. A comparison on microbial conditions between traditional dairy products sold in Karak and same products produced by modern dairies. Pakistan Journal of Nutrition 4.5 (2005): 345 - 348. DOI: https://doi.org/10.3923/pjn.2005.345.348

Mungai, E. A., Behravesh, C., & Gould, L. Increased Outbreaks Associated with Nonpasteurized Milk, United States, 2007–2012.Emerging Infectious Diseases, 21.1 (2015): 119-122. https://dx.doi.org/10.3201/eid2101.140447. DOI: https://doi.org/10.3201/eid2101.140447

Disassa, N., Sibhat, B., Mengistu, S., Muktar, Y., &Belina, D. Prevalence and antimicrobial susceptibility pattern of E. coli O157: H7 isolated from traditionally marketed raw cow milk in and around Asosa town, western Ethiopia. Veterinary medicine international, (2017).

Vahedi, M., Nasrolahei, M., Sharif, M., &Mirabi, A. M. Bacteriological study of raw and unexpired pasteurized cow's milk collected at the dairy farms and supermarkets in Sari city in 2011. Journal of preventive medicine and hygiene, 54.2 (2013): 120.

United States Food and Drug Administration. Bacteriological Analytical Manual. Washington, D.C: Association of Official Analytical Chemists. (2002). Retrieved from: https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm064948.htm

Welearegay, H., Yilma, Z., &Tekle-Giorgis, Y. Hygienic practices and microbiological quality of raw milk produced under different farm size in Hawassa, southern Ethiopia. Agricultural Research and Reviews, 1.4 (2012): 132-142.

Wallace, R. L. Bacteria count in raw milk. Dairy Cattle. Management, 1(2009): 4

White, C. How Pasteurization Works. (2019). Retrieved from: https://science.howstuffworks.com/life/cellular-microscopic/pasteurization5.htm

Home and Garden Information Centre. Safe Handling of Milk & Dairy Products. (2007). Retrieved from: https://hgic.clemson.edu/factsheet/safe-handling-of-milk-dairy-products/

Gwandu, S. H., Nonga, H. E., Mdegela, R. H., Katakweba, A. S., Suleiman, T. S., &Ryoba, R. Assessment of raw cow milk quality in smallholder dairy farms in Pemba Island Zanzibar, Tanzania. Veterinary medicine international. (2018). DOI: https://doi.org/10.1155/2018/1031726

LeJeune, J. T., Besser, T. E., & Hancock, D. D. Cattle Water Troughs as Reservoirs of Escherichia coli O157. Appl. Environ. Microbiol. 67.7(2001): 3053-3057. DOI: https://doi.org/10.1128/AEM.67.7.3053-3057.2001

Faith, N. G., Shere, J. A., Brosch, R., Arnold, K. W., Ansay, S. E., Lee, M. S. & Kaspar, C. W. Prevalence and clonal nature of Escherichia coli O157: H7 on dairy farms in Wisconsin. Appl. Environ. Microbiol. 62.5 (1996): 1519-1525. DOI: https://doi.org/10.1128/AEM.62.5.1519-1525.1996

Farm It. What happens if you don’t milk cows? (2019). Retrieved from: https://farmityourself.com/what-happens-if-you-dont-milk-cows/

Persaud, S., Mohamed-Rambaran, P., Wilson, A., James, C. and Indar, L. Determining the Community Prevalence of Acute Gastrointestinal Illness and Gaps in Surveillance of Acute Gastroenteritis and Foodborne Diseases in Guyana. Journal of Health, Population and Nutrition. 31.4 Suppl 1 (2013): S57.

How to Cite
Singh, R., Singh, D., Da Silva, P., & Subramanian, G. (2020). EVALUATION OF THE MICROBIAL LOAD AND IDENTIFICATION OF POSSIBLE SOURCES OF CONTAMINATION OF RAW COW’S MILK IN JOANNA, BLACK BUSH POLDER, REGION 6, GUYANA. International Journal of Research -GRANTHAALAYAH, 8(5), 22-31. https://doi.org/10.29121/granthaalayah.v8.i5.2020.44