CD24 GENE POLYMORPHISMS IN EGYPTIAN PATIENTS WITH COLORECTAL CANCER
Keywords:CD24,, Colorectal Cancer, Single Nucleotide Polymorphism, Carcinogenesis
Introduction: Early detection of colorectal cancer (CRC) is the key to reduce its related morbidity and mortality. Methods: One hundred CRC patients were included in this study, they were admitted at Mansoura Gastroenterology Center, Mansoura University and diagnosis was confirmed by pathology.100 apparently healthy individuals were served as the control group.CD24 (P170 C/T) and CD24 (P-534 A/C) single nucleotide polymorphisms (SNPs) were analyzed using polymerase chain reaction–restriction fragment length polymorphism (PCR/RFLP). Results: Regarding CD24 P170 C/T SNP, no statistically significant association was found between genotypes, alleles and risk of CRC. While, P-534 A/C; AC, CC, AC+CC genotypes and C allele showed significantly higher frequency in cases when compared to controls. CC and TC haplotypes were significantly associated with risk of CRC when compared to control group. Multivariate logistic regression analysis revealed that higher CEA and P-534 A/C (AC+CC) were considered as independent predictors of higher CRC stages. Conclusion: The current study observed an association between CD24 P-543 A/C SNP and the risk of developing CRC in Egyptian subjects.
Abou-Zeid A A, Khafagy W, Marzouk D M, et al. (2002) Colorectal cancer in Egypt. Diseases of the colon and rectum. 45: 1255-1260. Retrieved from https://doi.org/10.1007/s10350-004-6401-z DOI: https://doi.org/10.1007/s10350-004-6401-z
Aljebreen A M. (2007) Clinico-pathological patterns of colorectal cancer in Saudi Arabia: younger with an advanced stage presentation. Saudi journal of gastroenterology: official journal of the Saudi Gastroenterology Association; 13: 84-87. Retrieved from https://doi.org/10.4103/1319-3767.32183 DOI: https://doi.org/10.4103/1319-3767.32183
Amin M B, Greene F L, Edge S B, et al. (2017) The Eighth Edition AJCC Cancer Staging Manual: Continuing to build a bridge from a population-based to a more "personalized" approach to cancer staging. CA Cancer J Clin. 67: 93-99. Retrieved from https://doi.org/10.3322/caac.21388 DOI: https://doi.org/10.3322/caac.21388
Binefa G, Rodríguez-Moranta F, Teule A et al. (2014) Colorectal cancer: from prevention to personalized medicine. World journal of gastroenterology. 20: 6786-6808. Retrieved from https://doi.org/10.3748/wjg.v20.i22.6786 DOI: https://doi.org/10.3748/wjg.v20.i22.6786
Bray F, Ferlay J, Soerjomataram I, et al. (2018) Global cancer statistics: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018; 68: 394-424. Retrieved from https://doi.org/10.3322/caac.21492 DOI: https://doi.org/10.3322/caac.21492
Buck K, Hug S, Seibold P, et al. (2013) CD24 polymorphisms in breast cancer: impact on prognosis and risk. Breast cancer research and treatment. 137: 927-937. Retrieved from https://doi.org/10.1007/s10549-012-2325-9 DOI: https://doi.org/10.1007/s10549-012-2325-9
Corner C, Bryant L, Chapman C, et al. (2010) High-dose-rate afterloading intraluminal brachytherapy for advanced inoperable rectal carcinoma. Brachytherapy; 9: 66-70. Retrieved from https://doi.org/10.1016/j.brachy.2009.07.004 DOI: https://doi.org/10.1016/j.brachy.2009.07.004
Diaz-Gallo L M, Medrano L M, Gómez-García M, et al. (2011) Analysis of the influence of two CD24 genetic variants in Crohn's disease and ulcerative colitis. Hum Immunol. 72: 969-972. Retrieved from https://doi.org/10.1016/j.humimm.2011.05.028 DOI: https://doi.org/10.1016/j.humimm.2011.05.028
Fan Y, Zhang X, Yang Z-H, et al. (2013) The polymorphisms of osteopontin gene and plasma osteopontin protein levels with susceptibility to colorectal carcinoma. DNA and cell biology; 32: 594-600. Retrieved from https://doi.org/10.1089/dna.2013.2090 DOI: https://doi.org/10.1089/dna.2013.2090
Gado A, Ebeid B, Abdelmohsen A et al. (2014) Colorectal cancer in Egypt is commoner in young people: Is this cause for alarm? Alexandria Journal of Medicine; 50: 197-201. Retrieved from https://doi.org/10.1016/j.ajme.2013.03.003 DOI: https://doi.org/10.1016/j.ajme.2013.03.003
Gerger A, Zhang W, Yang D, et al. (2011) Common Cancer Stem Cell Gene Variants Predict Colon Cancer Recurrence. Clinical cancer research : an official journal of the American Association for Cancer Research; 17: 6934-6943. Retrieved from https://doi.org/10.1158/1078-0432.CCR-11-1180 DOI: https://doi.org/10.1158/1078-0432.CCR-11-1180
Giovannucci E. (2002) Modifiable risk factors for colon cancer. Gastroenterology clinics of North America; 31: 925-943. Retrieved from https://doi.org/10.1016/S0889-8553(02)00057-2 DOI: https://doi.org/10.1016/S0889-8553(02)00057-2
Gonzalez C A and Riboli E. (2010) Diet and cancer prevention: Contributions from the European Prospective Investigation into Cancer and Nutrition (EPIC) study. European journal of cancer; 46: 2555-2562. Retrieved from https://doi.org/10.1016/j.ejca.2010.07.025 DOI: https://doi.org/10.1016/j.ejca.2010.07.025
Horiguchi K, Toi M, Horiguchi S-I, et al. (2010) Predictive value of CD24 and CD44 for neoadjuvant chemotherapy response and prognosis in primary breast cancer patients. Journal of medical and dental sciences; 57: 165-175. Retrieved from https://doi.org/10.1016/j.ejcsup.2011.02.010 DOI: https://doi.org/10.1016/j.ejcsup.2011.02.010
Huang X L, Xu D H, Wang G P, et al. (2015) Associations between CD24 gene polymorphisms and inflammatory bowel disease: A meta-analysis. World J Gastroenterol; 21: 6052-6059. Retrieved from https://doi.org/10.3748/wjg.v21.i19.6052 DOI: https://doi.org/10.3748/wjg.v21.i19.6052
Jia Z F, Wang L Z, Cao X Y, et al. (2016) CD24 genetic variants contribute to overall survival in patients with gastric cancer. World J Gastroenterol. 22: 2373-2382. Retrieved from https://doi.org/10.3748/wjg.v22.i7.2373 DOI: https://doi.org/10.3748/wjg.v22.i7.2373
Jover R, Zapater P, Castells A, et al. (2006) Mismatch repair status in the prediction of benefit from adjuvant fluorouracil chemotherapy in colorectal cancer. Gut; 55: 848-855. Retrieved from https://doi.org/10.1136/gut.2005.073015 DOI: https://doi.org/10.1136/gut.2005.073015
Ke J, Wu X, Wu X, et al. (2012) Subpopulations of CD24⁺ cells in colon cancer cell lines possess stem cell characteristics. Neoplasma; 59: 282-288. Retrieved from https://doi.org/10.4149/neo_2012_036 DOI: https://doi.org/10.4149/neo_2012_036
Kim N H, Lee M Y, Park J H, et al. (2017) Serum CEA and CA 19-9 Levels are Associated with the Presence and Severity of Colorectal Neoplasia. Yonsei Med J; 58: 918-924. Retrieved from https://doi.org/10.3349/ymj.2017.58.5.918 DOI: https://doi.org/10.3349/ymj.2017.58.5.918
Kraus S R, Naumov I, Kazanov D, et al. (2010) Abstract 2902: A simple blood test, evaluating the level of CD24 protein, can detect subjects with colorectal adenomas and adenocarcinomas. Cancer Research; 70: Retrieved from https://doi.org/10.1158/1538-7445.AM10-2902 DOI: https://doi.org/10.1158/1538-7445.AM10-2902
Kraus S, Shapira S, Kazanov D, et al. (2015) Predictive Levels of CD24 in Peripheral Blood Leukocytes for the Early Detection of Colorectal Adenomas and Adenocarcinomas. Dis Markers; 916098. Retrieved from https://doi.org/10.1155/2015/916098 DOI: https://doi.org/10.1155/2015/916098
Kristiansen G, Sammar M and Altevogt P. (2014) Tumour biological aspects of CD24, a mucin-like adhesion molecule. J Mol Histol; 35: 255-262. Retrieved from https://doi.org/10.1023/B:HIJO.0000032357.16261.c5 DOI: https://doi.org/10.1023/B:HIJO.0000032357.16261.c5
Kwon E M, Salinas C A, Kolb S, et al. (2011) Genetic polymorphisms in inflammation pathway genes and prostate cancer risk. Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology; 20: 923-933. Retrieved from https://doi.org/10.1158/1055-9965.EPI-10-0994 DOI: https://doi.org/10.1158/1055-9965.EPI-10-0994
Ladabaum U, Dominitz J A, Kahi C et al. (2020) Strategies for Colorectal Cancer Screening. Gastroenterology; 158: 418-432. Retrieved from https://doi.org/10.1053/j.gastro.2019.06.043 DOI: https://doi.org/10.1053/j.gastro.2019.06.043
Li D, Zheng L, Jin L, et al. (2009) CD24 polymorphisms affect risk and progression of chronic hepatitis B virus infection. Hepatology; 50: 735-742. Retrieved from https://doi.org/10.1002/hep.23047 DOI: https://doi.org/10.1002/hep.23047
Lisiansky V, Kraus S, Naumov I, et al. (2014) Role of CD24 polymorphisms in the susceptibility to inflammatory bowel disease. The International journal of biological markers; 29: e62-e68. Retrieved from https://doi.org/10.5301/jbm.5000072 DOI: https://doi.org/10.5301/jbm.5000072
McFarlane M E C, Rhoden A, Fletcher P R et al. (2004) Cancer of the colon and rectum in a Jamaican population: diagnostic implications of the changing frequency and subsite distribution. The West Indian medical journal; 53: 170-173. Retrieved from https://europepmc.org/article/med/15352746
Nadeem M, Karunanand B, Bhat S et al. (2019) CEA levels in colorectal carcinoma, correlation with the tumor staging, and CEA as independent predictor of prognosis of colorectal cancers; 3: 210-213.
Parkin D M, Bray F, Ferlay J et al. (2005) Global cancer statistics, 2002. CA: a cancer journal for clinicians; 55: 74-108. Retrieved from https://doi.org/10.3322/canjclin.55.2.74 DOI: https://doi.org/10.3322/canjclin.55.2.74
Polat E, Duman U, Duman M, et al. (2014) Diagnostic value of preoperative serum carcinoembryonic antigen and carbohydrate antigen 19-9 in colorectal cancer. Current oncology; 21: e1-e7. Retrieved from https://doi.org/10.3747/co.21.1711 DOI: https://doi.org/10.3747/co.21.1711
Ribeiro K B, da Silva Zanetti J, Ribeiro-Silva A, et al. (2016) KRAS mutation associated with CD44/CD166 immunoexpression as predictors of worse outcome in metastatic colon cancer. Cancer Biomark; 16: 513-521. Retrieved from https://doi.org/10.3233/CBM-160592 DOI: https://doi.org/10.3233/CBM-160592
Sadot E, Kraus S, Stein M, et al. (2014) CD24 gene polymorphism--a novel prognostic factor in esophageal cancer. The International journal of biological markers; 29: e49-e54. Retrieved from https://doi.org/10.5301/jbm.5000071 DOI: https://doi.org/10.5301/jbm.5000071
Sheng L and Shui Y. (2011) Clusters of differentiation 24 polymorphism and hepatocellular carcinoma. Hepatology; 54: 2273; author reply 2273-2274. Retrieved from https://doi.org/10.1002/hep.24676 DOI: https://doi.org/10.1002/hep.24676
Siegel R L, Miller K D, Goding Sauer A, et al. (2020) Colorectal cancer statistics, 2020. CA Cancer J Clin; 70: 145-164. Retrieved from https://doi.org/10.3322/caac.21601 DOI: https://doi.org/10.3322/caac.21601
Soliman A S, Bondy M L, El-Badawy S A, et al. (2001) Contrasting molecular pathology of colorectal carcinoma in Egyptian and Western patients. British journal of cancer; 85: 1037-1046. Retrieved from https://doi.org/10.1054/bjoc.2001.1838 DOI: https://doi.org/10.1054/bjoc.2001.1838
Song M, Hu F B, Spiegelman D, et al. (2015) Adulthood Weight Change and Risk of Colorectal Cancer in the Nurses' Health Study and Health Professionals Follow-up Study. Cancer prevention research; 8: 620-627. Retrieved from https://doi.org/10.1158/1940-6207.CAPR-15-0061 DOI: https://doi.org/10.1158/1940-6207.CAPR-15-0061
Spelt L, Sasor A, Ansari D, et al. (2018) The Prognostic Role of Cancer Stem Cell Markers for Long-term Outcome After Resection of Colonic Liver Metastases. Anticancer Res; 38: 313-320. Retrieved from https://doi.org/10.21873/anticanres.12224 DOI: https://doi.org/10.21873/anticanres.12224
Stojkovic Lalosevic M, Stankovic S, Stojkovic M, et al. (2017) Can preoperative CEA and CA19-9 serum concentrations suggest metastatic disease in colorectal cancer patients? Hellenic journal of nuclear medicine; 20: 41-45. Retrieved from https://www.researchgate.net/profile/Milica-Stojkovic-Lalosevic/publication/315476362_Can_preoperative_CEA_and_CA19-9_serum_concentrations_suggest_metastatic_disease_in_colorectal_cancer_patients/links/5b30bea60f7e9b0df5c742f3/Can-preoperative-CEA-and-CA19-9-serum-concentrations-suggest-metastatic-disease-in-colorectal-cancer-patients.pdf
Tomašević R, Milosavljević T, Stojanović D, et al. (2016) Predictive Value of Carcinoembryonic and Carbohydrate Antigen 19-9 Related to Some Clinical, Endoscopic and Histological Colorectal Cancer Characteristics. Journal of medical biochemistry; 35: 324-332. Retrieved from https://doi.org/10.1515/jomb-2016-0014 DOI: https://doi.org/10.1515/jomb-2016-0014
Wang J, Wang X, Yu F, et al. (2015) Combined detection of preoperative serum CEA, cancer patients. International journal of clinical and experimental pathology; 8: 14853-14863.
Wang L, Liu R, Li D, et al. (2012) A hypermorphic SP1-binding CD24 variant associates with risk and progression of multiple sclerosis. American journal of translational research; 4: 347-356. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426393/
Yan S, Xu D, Jiang T, et al. (2014) CD24 single nucleotide polymorphisms and cancer risk. Tumor Biology; 35. Retrieved from https://doi.org/10.1007/s13277-014-2127-2 DOI: https://doi.org/10.1007/s13277-014-2127-2
Yeo M K, Lee Y M, Seong I O, et al. (2016) Up-regulation of Cytoplasmic CD24 Expression Is Associated with Malignant Transformation but Favorable Prognosis of Colorectal Adenocarcinoma. Anticancer Res; 36: 6593-6598. Retrieved from https://doi.org/10.21873/anticanres.11264 DOI: https://doi.org/10.21873/anticanres.11264
Youssef M R, Attia Z I, El-Baz R A, et al. (2017) Genetic polymorphisms of NFκB1-94ins/delATTG and NFκBIA-881A/G genes in Egyptian patients with colorectal cancer. Familial cancer; 16: 517-524. Retrieved from https://doi.org/10.1007/s10689-017-9992-y DOI: https://doi.org/10.1007/s10689-017-9992-y
Youssef M, Ewieda H, Ali A, et al. (2013) Comparison between CEA, CA 19-9 and CA 72-4 in Patients with Colon Cancer. International Journal of Tumor Therapy; 2: 26-34.
Zhang Y, Li B, Zhang X, et al. (2017) CD24 is a genetic modifier for risk and progression of prostate cancer. Mol Carcinog; 56: 641-650. Retrieved from https://doi.org/10.1002/mc.22522 DOI: https://doi.org/10.1002/mc.22522
How to Cite
Copyright (c) 2021 Eman Saber Elhennawy, Abeer Mesbah Abd-El Hamid, Tharwat Saad Kandil, Mohammed Mohammed El-Sayed El-Arman, Rasha Elzehery
This work is licensed under a Creative Commons Attribution 4.0 International License.
With the licence CC-BY, authors retain the copyright, allowing anyone to download, reuse, re-print, modify, distribute, and/or copy their contribution. The work must be properly attributed to its author.
It is not necessary to ask for further permission from the author or journal board.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.