• Lingming Kong Prevention and Treatment Center for Psychological Diseases, No. 904 Hospital of Chinese PLA, Changzhou 213003, Jiangsu, China
  • Xiaoli Zhu Prevention and Treatment Center for Psychological Diseases, No. 904 Hospital of Chinese PLA, Changzhou 213003, Jiangsu, China
  • Wei Niu Department of Rehabilitation, No.904 Hospital of Chinese PLA, Changzhou, Jiangsu 213003, China
  • Liyi Zhang Prevention and Treatment Center for Psychological Diseases, No. 904 Hospital of Chinese PLA, Changzhou 213003, Jiangsu, China




GAD, MicroRNA, Social Support, Personality


To explore the differential expression of microRNA (miRNA, miR-) and its correlation with social support and personality in Generalized Anxiety Disorder (GAD) patients. A total of 80 GAD patients and 80 control participants were selected from our serial study samples to be tested for microarray analysis, and real time quantitative Polymerase Chain Reaction (qRT-PCR) verification was carried out in both patient group and control group. Correlation between miRNA expression and social support were also carried out. According to microarray analysis, 6 miRNAs were differentially expressed between patient group and control group, with miR-4505, miR-4484, miR-4674, miR-501-3p and miR-663 up-regulated, and miR-1301 down-regulated; qPCR verification revealed that 5 miRNAs (miR-1301, miR-4484, miR-4674,  miR-501-3p, miR-663, miR-4505) were significantly up-regulated and the expression of miRNA-1301 down-regulated; the expression of miRNA-1301 was significantly related to social support in GAD patients. In conclusions, comprehensive factors, including miRNA regulation, social support may be involved into the aetiology of GAD.


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Abbas, J., Aqeel, M., Abbas, J., Shaher, B., Jaffar, A., Sundas, J., & Zhang, W. (2019). The moderating role of social support for marital adjustment, depression, anxiety, and stress: Evidence from Pakistani working and nonworking women. J Affect Disord, 244, 231-238. Retrieved from https://doi.org/10.1016/j.jad.2018.07.071 DOI: https://doi.org/10.1016/j.jad.2018.07.071

Arzua, T., Jiang, C., Yan, Y. S., & X. W. (2021). The importance of non-coding RNAs in environmental stress-related developmental brain disorders: A systematic review of evidence associated with exposure to alcohol, anesthetic drugs, nicotine, and viral infections. Neurosci Biobehav Rev, 1283, 63-647. Retrieved from https://doi.org/10.1016/j.neubiorev.2021.06.033 DOI: https://doi.org/10.1016/j.neubiorev.2021.06.033

Baxter, A., Scott, K., Vos, T., & Whiteford, H. (2013). Global prevalence of anxiety disorders: a systematic review and meta-regression. Psychol med, 43, 897-910. Retrieved from https://doi.org/10.1017/S003329171200147X DOI: https://doi.org/10.1017/S003329171200147X

Domschke, K., Schiele, M. A., & Romanos, M. (2021). Prevention of anxiety disorders. Nervenarzt, 92, 450-456. Retrieved from https://doi.org/10.1007/s00115-020-01045-1 DOI: https://doi.org/10.1007/s00115-020-01045-1

Ebrahimi, S. O., Reiisi, S., & Shareef, S. (2020). MiRNAs, oxidative stress, and cancer: A comprehensive and updated review. J Cell Physiol, 235, 8812-8825. Retrieved from https://doi.org/10.1002/jcp.29724 DOI: https://doi.org/10.1002/jcp.29724

Eilertse, M. E. B., Eilegård, A., Steineck, G., Nyberg, T., & Kreicbergs, U. (2013). Impact of Social Support on Bereaved Siblings' Anxiety A Nationwide Follow-Up. J Pediatr Oncol Nurs, 30, 301-310. Retrieved from https://doi.org/10.1177/1043454213513838 DOI: https://doi.org/10.1177/1043454213513838

Fagg, J., Curtis, S., Stansfeld, S. A., Cattell, V., Tupuola, A. M., & Arephin, M. (2008). Area social fragmentation, social support for individuals and psychosocial health in young adults: Evidence from a national survey in England. Soc Sci Med, 66, 242-254. Retrieved from https://doi.org/10.1016/j.socscimed.2007.07.032 DOI: https://doi.org/10.1016/j.socscimed.2007.07.032

Fitzpatrick, S., Bailey, K., & Rizvi, S. L. (2020). Changes in Emotions Over the Course of Dialectical Behavior Therapy and the Moderating Role of Depression, Anxiety, and Posttraumatic Stress Disorder. Behav Ther, 51, 946-957. Retrieved from https://doi.org/10.1016/j.beth.2019.12.009 DOI: https://doi.org/10.1016/j.beth.2019.12.009

Hoffart, A., Johnson, S. U., & Ebrahimi, O. V. (2021). The network of stress-related states and depression and anxiety symptoms during the COVID-19 lockdown. J Affect Disord, 294, 671-678. Retrieved from https://doi.org/10.1016/j.jad.2021.07.019 DOI: https://doi.org/10.1016/j.jad.2021.07.019

Kennedy, A. C., Bybee, D., Sullivan, C. M., & Greeson, M. (2009). The effects of community and family violence exposure on anxiety trajectories during middle childhood: The role of family social support as a moderator. J Clin Child Adolescent Psychol, 38, 365-379. Retrieved from https://doi.org/10.1080/15374410902851713 DOI: https://doi.org/10.1080/15374410902851713

Malan-Müller, S., Hemmings, S. M. J., & Seedat, S. (2013). Big effects of small RNAs: a review of microRNAs in anxiety. Molecular neurobiology, 47, 726-739. Retrieved from https://doi.org/10.1007/s12035-012-8374-6 DOI: https://doi.org/10.1007/s12035-012-8374-6

Menezes, I. C., von Werne, B.C., Lacchini, R., & Juruena, M. F. (2019). Genetic biomarkers for differential diagnosis of major depressive disorder and bipolar disorder: A systematic and critical review. Behav Brain Res, 357-358, 29-38. Retrieved from https://doi.org/10.1016/j.bbr.2018.01.008 DOI: https://doi.org/10.1016/j.bbr.2018.01.008

Qi, M., Zhou, S. J., Guo, Z. C., Zhang, L. G., Min, H. J., Li, X. M., & Chen, J.X. (2020). The effect of social support on mental health in Chinese adolescents during the outbreak of COVID-19. J Adolescent Health, 67, 514-518. Retrieved from https://doi.org/10.1016/j.jadohealth.2020.07.001 DOI: https://doi.org/10.1016/j.jadohealth.2020.07.001

Ren, Y. J., & Li, M. L. (2020). Influence of physical exercise on social anxiety of left-behind children in rural areas in China: The mediator and moderator role of perceived social support. J Affect Disord, 266, 223-229. Retrieved from https://doi.org/10.1016/j.jad.2020.01.152 DOI: https://doi.org/10.1016/j.jad.2020.01.152

Riem, M. M. E., Kunst, L. E., & Kop, W. J. (2021). Intranasal oxytocin and the stress-buffering effects of social support during experimentally induced pain: The role of attachment security. J Affect Disord, 278, 149-156. Retrieved from https://doi.org/10.1016/j.jad.2020.09.057 DOI: https://doi.org/10.1016/j.jad.2020.09.057

Segaran, R. C., Chan, L. Y., Wang, H., Sethi, G., & Tang, F. R. (2021). Neuronal development-related miRNAs as biomarkers for Alzheimer's disease, depression, schizophrenia and ionizing radiation exposure. Curr Med Chem, 28, 19-52. Retrieved from https://doi.org/10.2174/0929867327666200121122910 DOI: https://doi.org/10.2174/0929867327666200121122910

Song, H. T., Sun, X. Y., Zhang, L. Y., Zhao, L., Guo, Z. M., Fan, H. M., Zhong, A. F., Niu, W., Dai, Y. H., Zhang, L. Y., Shi, Z., Liu, X. P., & Lu, J. (2014). A preliminary analysis of association between the down-regulation of microRNA-181b expression and symptomatology improvement in schizophrenia patients before and after antipsychotic treatment. J Psychiatr Res, 54, 134-140. Retrieved from https://doi.org/10.1016/j.jpsychires.2014.03.008 DOI: https://doi.org/10.1016/j.jpsychires.2014.03.008

Strell-Zimonyi, F., Kovacs, A., & Miklosi, M. (2020). Developmental psychopathology perspective of social anxiety disorder. Neuropsychopharmacol Hung, 22, 1-10. Retrieved from https://pubmed.ncbi.nlm.nih.gov/33055290/

Tirone, V., Orlowska, D., Lofgreen, A. M., Blais, R. K., Stevens. N. R., Klassen, B., Held, P., & Zalta, A. K. (2021). The association between social support and posttraumatic stress symptoms among survivors of betrayal trauma: a meta-analysis. Eur J Psychotraumatol, 12, 10.1080/20008198.2021, in press. Retrieved from https://doi.org/10.1080/20008198.2021.1883925 DOI: https://doi.org/10.1080/20008198.2021.1883925

Weiss, S.J., Flynn, H., Christian, L., Hantsoo, L., di Scalea, T. L., Kornfield, S. L., Muzik, M., Simeonova, D. I., Cooper, B. A., Strahm, A., Deligiannidis, K. M. (2021). Symptom profiles of women at risk of mood disorders: A latent class analysis. J Affect Disord, 295, 139-147. Retrieved from https://doi.org/10.1016/j.jad.2021.08.013 DOI: https://doi.org/10.1016/j.jad.2021.08.013

Zhou, L. S., Zhu, Y. Y., Chen, W. Y., & Tang, Y. P. (2021). Emerging role of microRNAs in major depressive disorder and its implication on diagnosis and therapeutic response. J Affect Disord, 286, 80-86. Retrieved from https://doi.org/10.1016/j.jad.2021.02.063 DOI: https://doi.org/10.1016/j.jad.2021.02.063

Zhou, S. J,, Wang, L. L., Yang, R., Yang, X. J., Zhang, L. G., Guo, Z. C., Chen, J. C., Wang, J. Q., & Chen, J. X. (2020). Sleep problems among Chinese adolescents and young adults during the coronavirus-2019 pandemic. Sleep Med, 74, 39-47. Retrieved from https://doi.org/10.1016/j.sleep.2020.06.001 DOI: https://doi.org/10.1016/j.sleep.2020.06.001




How to Cite

Kong, L., Zhu, X., Niu, W., & Zhang, L. (2021). DIFFERENTIAL EXPRESSION OF MICRORNA IN GENERALIZED ANXIETY DISORDER AND ITS ASSOCIATION WITH SOCIAL SUPPORT. International Journal of Research -GRANTHAALAYAH, 9(9), 286–292. https://doi.org/10.29121/granthaalayah.v9.i9.2021.4265

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