INFLUENCE OF 627 NM WAVELENGTH LIGHT EMITTING DIODE PHOTOTHERAPY ON SECONDARY INTENTION WOUND HEALING

Patricia Pacheco Tyski Suckow; Ana Carolina Dorigoni Bini; Andressa Panegalli Hosni; Andressa Leticia Miri; Maria Elvira Ribeiro Cordeiro; Larissa Sakis Bernardi; Carolina Panis; Angela Dubiela Julik; Eliane Gonçalves de Jesus Fonseca; Afonso Shiguemi Inoue Salgado; Ivo Ilvan Kerppers; Paulo Renato de Oliveira

doi:10.29121/granthaalayah.v9.i6.2021.3983

Authors

Patricia Pacheco Tyski Suckow Post Graduation Program in Pharmaceutical Sciences, Department of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil https://orcid.org/0000-0002-5022-7612
Ana Carolina Dorigoni Bini Post Graduation Program In Pharmaceutical Sciences, Department Of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil https://orcid.org/0000-0003-1717-9249
Andressa Panegalli Hosni Post Graduation Program In Pharmaceutical Sciences, Department Of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil https://orcid.org/0000-0002-2980-9773
Andressa Leticia Miri Post Graduation Program in Rehabilitation Sciences, State University of Londrina, Londrina, PR, Brazil
Maria Elvira Ribeiro Cordeiro Pharmacy Student. Department of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil
Larissa Sakis Bernardi Post Graduation Program in Pharmaceutical Sciences, Department of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil https://orcid.org/0000-0003-0864-2262
Carolina Panis Professor Of Immunology At The State University Of West Paraná. Head Of The Laboratory Of Tumor Biology, Performing Research Focused On Tumor Biology, Inflammation And Redox Signaling https://orcid.org/0000-0002-0104-4369
Angela Dubiela Julik Post Graduation Program in Community Development, Midwest State University - UNICENTRO, Guarapuava, Brazil https://orcid.org/0000-0001-7375-6771
Eliane Gonçalves de Jesus Fonseca Post Graduation Program in Pharmaceutical Sciences, Department of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil
Afonso Shiguemi Inoue Salgado Phd In Biomedical Engineering, Chief Science Officer Of Natural Quanta LLC, And The Integrative Health School, Orlando, FL
Ivo Ilvan Kerppers Laboratory of Neuroanatomy and Neurophysiology, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil
Paulo Renato de Oliveira Post Graduation Program in Pharmaceutical Sciences, Department of Pharmacy, Midwest State University - UNICENTRO, Guarapuava, PR, Brazil https://orcid.org/0000-0003-0466-1327

DOI:

https://doi.org/10.29121/granthaalayah.v9.i6.2021.3983

Keywords:

Wound Healing, LED, Phototherapy, Grow Factor, Transformation Factor, Secondary Intention

Abstract [English]

Background: The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing. Methods: The evaluations were performed using a dorsal puncture model and the sample consisted of 120 Wistar rats, randomly divided into LED groups submitted to 3, 7, 14 and 21 irradiation treatment for 100 s, 7 J / cm2, and control groups. Histological analysis, immunohistochemistry and contraction of the lesion were performed. Results: LED irradiation led to effective wound regeneration results. Similar effects were obtained with vascular, exudative, and proliferative phenomena. VEGF and TGF-β1 levels were increased in LED-treated groups with initial treatments. Collagen analysis revealed that LED-treated groups levels were increased on days 14- and 21 of the treatments. An acceleration in tissue regeneration was observed in LED treated groups. Macroscopically visible results showing a complete healing process was evident after 14 days of treatment. Conclusion: 627 nm LED irradiation effectively accelerated wound healing leading complete closure of the skin in a shorter time compared to control group.