IMMEDIATE APPLY COST-EFFECTIVE EASILY PREPARABLE- AVAILABLE 21STCENTURY POTENTIAL-AYURVEDIC-HERBAL- INTEGRATIVE-MEDICINAL-VACCINE OF COVID-19: ACHIEVED AGRICULTURE HEALTHCARE-SOCIO-ECONOMY SCIENCE TECHNOLOGY COMMUNICATION MECHANISM!

© 2021 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 227 IMMEDIATE APPLY COST-EFFECTIVE EASILY PREPARABLEAVAILABLE 21STCENTURY POTENTIAL-AYURVEDIC-HERBALINTEGRATIVE-MEDICINAL-VACCINE OF COVID-19: ACHIEVED AGRICULTURE HEALTHCARE-SOCIO-ECONOMY SCIENCE TECHNOLOGY COMMUNICATION MECHANISM!


RECENT PROBLEMS
The pandemic COVID19 effects on the civilization of human with health, travel, socio-economy and clinical research, and scientists, has been an tried to prepare cost-effective proper vaccines of COVID-19 [2] for the unusual viral pneumonia [3], forming the fifth endemic coronavirus, the health danger [4], and wildlife contains many closely related animal-human coronaviruses [5], genetic resistance [6], divergence, structural and the future evolution, adaptation, and spread [7], prevailing for a long time as an asymptomatic patients [8], analogous to war [9], and the WHO do research, making blueprint, diagnostics, vaccines, and therapeutics for this novel coronavirus [10].

IMPACT OF VIRUSES ON THE GLOBAL SOCIOECONOMY
To overcome the pandemic crisis, India depends on vegetables [11], okra, the oldest widely cultivated-oligo purpose traditional medicines [12], consumed in a variety of ways [13], for good source of superior nutritional quality, achieving India first in the world [13]. But the Root-Knot (RK) diseases, Yellow Vein Mosaic Virus (YVMV) disease-and Okra Enation Leaf Curl Virus (OELCV) disease of plants hamper production. The chemical pesticides control diseases, but they are expensive and environment unfriendly-health hazards [14], [15] and the geneticsresistance [16] to YVMV and OELCV in okra, and diversity of viruses affect the global economy [17], [18].

PREPARATION OF ROOT PROTEINS
Fresh roots of okra plants of 6-groups were collected and homogenized [56] forming extract, which was then used for centrifuged forming the supernatants, and were collected and transferred for lyophilization, and the powdery extract was stored (-200C) for confirmation of protein separation [54], [57].

DENSITOMETER SCANNING
Analysis of total soluble roots proteins separation was carried out essentially by the method of Laemmli [57], by using one-dimensional vertical sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with the modification as suggested by the LKB Instructional Manual (1986). A 10% separating gel and 5% stacking gel were used. Samples for the preparation of electrophoretic run were mixed with 2x treatment buffer, and the root protein

ANALYSIS OF RESIDUES TOXICITY
The ayurvedic-biomedicines; Artemisia nilagirica-extract-treated leaves of okra did not contain any toxic residue again. Table 1 confirms again that both treatment with the high diluted ayurvedic-biomedicines; Artemisia nilagiricaextract, at extremely low doses, on Root-Knot (RK) disease, and both the foliar diseases of okra plants; Yellow Vein Mosaic Virus (YVMV) and Okra Enation Leaf Curl Virus (OELCV), estimated before pretreatments (Day-0) and after last posttreatment (Day -30), in a field trial with the number of common-shared root-protein in relation to uninoculated untreated one (P<0.01 by 't'-test). All the ayurvedic-biomedicines; Artemisia nilagirica-extract pretreatment, remarkably decreased the 3-diseases, and all the plant's groups, average; the number of leaves, number of nematodes in soil, the biomass of shoots, and protein of fruits, are remarkably increased than inoculated untreated groups, but the mean of the biomass of roots, number of root galls and nematodes populations in roots, and root protein content percent, is greater in the inoculated untreated one again. It is confirmed that the commonshared root proteins are seven, the highest number, in healthy uninoculated untreated control plants, but the lowest number is one in the inoculated untreated one (Table 1 and Fig. 1) again. 'Day-0' -means before pretreatment. 'Day-30' -means after posttreatment. Table 2 and Fig 1; confirms the root protein's molecular weight (k) of the different okra plants, with both the treatments of high diluted ayurvedic-biomedicines; Artemisia nilagirica-extract, at extremely low doses, on Root-Knot disease, and Yellow Vein Mosaic Virus (YVMV) and Okra Enation Leaf Curl Virus (OELCV) foliar diseases of okra plants, and the root proteins of all groups confirmed by the increased number of proteins in the roots again; the lowest number of protein is 11 in the uninoculated untreated roots and highest number of proteins, are 18 in the pretreated-and 16 in the post-treated-ayurvedic-biomedicines; Artemisia nilagirica-extract, roots respectively again ( Table 2 and Fig. 1). It is also confirmed that 280k (280,000kDa) is the highest molecular weight protein, and 11k (11,000kDa) is the lowest molecular weight protein. Four is the lowest number of the new pathogenesis-related protein (PR-proteins) in the uninoculated untreated roots, and 15 and 14 are the highest number of the new pathogenesis-related proteins (PR-proteins) in the pretreated-and post-treated-ayurvedic-biomedicines; Artemisia nilagirica-extract, roots respectively again ( Table 2 and Fig. 1), and 14 is in the PR-proteins of inoculated untreated one again (Table 2 and Fig. 1).

APPLICATIONS OF SCIENCE AND TECHNOLOGY COMMUNICATIONS
It is confirmed by many ways like campaign, aware, discuss, arrange workshops and seminars, make news, and publish as abstract regarding the importance of ayurvedic-biomedicines against various pathogens, including COVID-19.

AYURVEDIC VACCINE PREPARATION
The high-diluted-ayurvedic-biomedicines; Artemisia nilagirica, the vaccine preparation for treatment, at extremely low doses, against COVID-19, confirms the future goal.

RESEARCH IN FUTURE
Here, the ayurvedic-biomedicines; Artemisia nilagirica-extract, at extremely low doses, with daily consumption of okra-vegetables are confirmed research in the future again.

DEFENSE ON PR-PROTEINS
It is already known that a plant ATP binding cassette-type transporter [70] involving in antifungal terpenoid for the disease resistance, or by the sharing of common antigens with its host plants [71] or by the pure compounds of acaciasides [72], [73], [74], [75], using against pathogens. The treatments with the highly-diluted ayurvedicbiomedicines; Artemisia nilagirica-extract, at extremely low doses, confirm the synthesis of many antigens-proteins, and the lowest number of the new PR-proteins is 4 in the uninoculated untreated roots and the highest number of the new PR-proteins is 15, inducing defense responses involving several pathogenesis-related proteins in which the naturally infected plant pathogens fail to tolerate like nematode extract [15], [20], [22], [76], [77], due to the antigenic-proteins similarity [71], which is proved from the inoculated untreated pretreatment okra plants, were directly using against diseases of plants and animals, the Artemisia nilagirica-extract, and improving immunity or defense responses for the production of new PR-proteins.

FROM GENOME BIOLOGY
It is well known that the human-genetic material; the 145-genes come from bacteria, other unicellular organisms, and viruses, and 17-as possible horizontal-gene transfer [82], the 96.2% identical genomic sequencing to a bat coronavirus, which shares a 79.5% sequence identity to SARS-CoV [82], [83], [84] dealing the structure and function of genetic material [85], [86], and the ten percent of the human genome making of virus-DNA [87], and the human endogenous retroviruses [88] which don't always require a body [89].

FROM GENETIC RESISTANCE
It is reported that genetic resistance to coronavirus infection according to those three host resistance mechanisms: genetic control [16] and the pandemic COVID-19 outbreak is due to SARS-CoV-2 responsible for the main protease, the key enzyme helping in antiviral drug formation [90].

FROM IMMUNE SYSTEM BLUEPRINT
The human body is attacked by a virus, the immune system starts to fight, creating a blueprint in the body, and remembering the germ to fight against re-infection by the viruses [91].

FROM THERAPEUTIC APPROACH
The NovavaxInc, use as epidemic vaccines of COVID-19 using their recombinant protein, the 'saponin-based'adjuvant with the "potent and well-tolerated effect" [97], [98]. Scientists are discovering novel inhibitor molecules against enzymes Mpro and ACE2, and developing the antiviral compounds against Coronavirus [99].

FROM SUCCESSFUL VACCINATION
The successful vaccination requires four components; Human Immunomics Initiative (HII) aims to decode the underlying mechanisms of the human immune system [100], following the guideline entitled "Vaccine-preventable diseases and vaccines" [10], [100], [101], for the "reduces wait time for emergency vaccination" [102] and randomizing the trials [103] with the candidates, and the Merck's new investments focus on two different COVID-19 vaccines for clinical trials [105].

SITUATIONS FOR IMMEDIATE USE OF DRUGS AGAINST COVID-19
Recently, the COVID-Toes [116], locust attack [117], excess deaths in lower-income [109], and humanitarian crisis [110], united the world for vaccine of the novel coronavirus; many groups (80) are working [111] in different stages of clinical trials [118]. In this situation, it is thought that the personalized medicines [119], for the production of vaccines, and to distribute free for all the population, and important producer, becoming the "bad example" [120] for the present treatments with the high-diluted ayurvedic-biomedicines; Artemisia nilagirica-extract-biomedical drugs, at an extremely low dose, should be immediately applied against pandemic COVID-19 for the beneficial clinical treatments, with no side effects [121], [122], [123], [124].
It is known that our body as a complex system, has the capacity for self-organization, emergence and selfsimilarity over global (overall health and wellbeing) and local (organ) levels of organization and these features are key for future research on the systemic healing that evolves over time during individualized emergency treatment with medicines; Artemisia nilagirica-extract [131], which is a complex nano-scale system involving multiple interconnected, interacting components, and emergent properties [132]. So, it is the most; cost-effective, easily prepare able, easily available, and helps in biodiversity conservations and green economy applications issues and easily applicable also with increasing immunity [128] and the high-diluted ayurvedic-biomedicines; Artemisia nilagiricaextract, is the best emergency applicable effective applicable treatment potential biomedicine [46], [101], [102], [104], [114] , [115] , [122], [123], [124], [125], against COVID-19. It is also studied the cost-effectiveness of emergency care interventions in low and middle-income countries like India [120], [121], [122], [123], [124].

VACCINE-III FOR ALL PATIENTS
The adjuvant 'Indian Wormwood' ayurvedic-biomedicines; -Artemisia nilagirica-extract [96], [113] maybe used with recombinant protein nanoparticle antigens derived from the coronavirus spike protein and combine these antigens with its adjuvant ayurvedic-biomedicines; Artemisia nilagirica-extract for the final formulation of the vaccine and it may be shown a "potent and well-tolerated effect" through stimulating the entry of antigen-presenting cells into the injection site and enhancing antigen presentation in local lymph nodes, boosting immune responses [31], [38], [39], [40], [41], [42], [43], [44], [45], [46], [97], [98], [113], [114], [115], [133]. In a letter as an e-mail, the  [133] and the readily available coronavirus spike proteins may be helped to use for vaccine preparation which may fight against "COVID Toes among kids: New symptom of novel coronavirus infection" [116]. Here, vaccination or treatments, is the use of remedies against diseases either earlier in an epidemic or given routinely to prevent diseases. When the latter is used it involves mostly the users just like any conventional vaccination which administers the antigen in an inactive state to gain immunity towards the disease and is given before the onset of disease or disease symptoms in an individual as a prevention rather than cure [122] and in a clinical study shows the efficiency of triple antibiotic mixture and propolis as intracanal medication in revascularization process in immature apex [122], [134]. It is obligatory that information to the ClinicalTrials.gov, the U.S. National Library of Medicine, to the National Institutes of Health or other agencies of the U.S. Federal Government [92], [100], [101], [127] and maybe easily emergencyapplicable drugs with increasing immunity [128].

SCIENCE AND TECHNOLOGY COMMUNICATIONS ECONOMY
Recently, the Coronavirus pandemic has adverse effects on education, especially on school education and characteristics, including research, academic programs, staff professional development, and jobs also [129]. At present, many groups (80) are working globally for vaccines for a human which normally takes years to develop. Currently, there are [111] potential vaccines for the SARS-CoV-2 which are in different stages of clinical trials [118] and a committee that makes vaccine use recommendations to the U.S. Centers for Disease Control and Prevention (CDC), suggesting they should be high on the list [138].

FUTURE RESEARCH OKRA AS A VACCINE
Here, the results fulfill the goal of a research title because the present treatments with -ayurvedic-biomedicines; Artemisia nilagirica-extract, at extremely low doses, with regular consumption of okra vegetables of need to justify future research ideas. It is known that social vaccines resist and change unhealthy social and economic structures and useful metaphor for health promotion [130]. It may be the most; cost-effective, easily-available, safe-edible and prepare able as well as and safe alternative to live replicating COVID-19 vaccines which restarts, a window of hope and opportunity opens for nations to green their recovery the 21st-century economy in ways that are clean, green, healthy, safe and more resilient, and there are many other ways future positive applications [139], [140], [141], [142], [143], [144], [145], [146], [147], [148], [149], [150], [151], [152], [153], [154].
• Overcome various medical complications.
• Proven for many pharmacological activities.
• Present of various effective phytochemical constitutions.
• No side effects and some vaccines are made from its phytoconstituents.
• Cost effectiveness, easily prepare-able, easily available, easily manufacture-able, equitable, marketable and supply-able etc.

CONCLUSIONS
The recent treatment confirms once again that the ayurvedic-medicines-prepared from the floweringmeristems of Artemisia nilagirica (Clarke) Pamp are very much effective in controlling different-plant-pathogens caused many-diseases, synthesizing many new-pathogenesis-related-proteins (PR-proteins), boosting their defense-response naturally against pathogens, and increased plants-growth and content of protein. So, it may be confirmed for "Immediate Apply Cost-Effective Easily Preparable-Available 21stCentury Potential-Ayurvedic-Herbal-Integrative-Medicinal-COVID-19-Vaccine: Achieved Green-Socio-Economic Healthcare Science Technology Communication Mechanism", by boosting-immunity. And it will be best side-effect-free potential-ayurvedic-COVID-19-Vaccine due to an ultra-diluted-low-dose, and globally develop all aspects in the scientific-basis of the ayurvedicbiomedicines, clinical, physical, chemical, biological, and physiological effects, with relative molecular mass, and the sincere hope that the crisis of human civilization will soon be over and also the whole world may retain in newnormal or old-forms by fighting threatening the recovery against the COVID-19.

SOURCES OF FUNDING
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.