HOMEOPATHIC MEDICINES-AAKASHMONI WILL BE THE BEST VACCINE AGAINST COVID-19: ENRICHING AGRICULTURE SCIENCE AND TECHNOLOGY COMMUNICATION MECHANISM APPLICATION ISSUES!

© 2020 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 333 HOMEOPATHIC MEDICINES-AAKASHMONI WILL BE THE BEST VACCINE AGAINST COVID-19: ENRICHING AGRICULTURE SCIENCE AND TECHNOLOGY COMMUNICATION MECHANISM APPLICATION ISSUES!


INTRODUCTION
In television or newspapers or social media is always informing that there is currently no proper effective treatment for novel coronavirus disease-COVID-2019 which was first detected on 31st December 2020 in Wuhan City, Hubei Province of China, a cluster of cases of pneumonia of unknown cause, was informed by WHO and was identified as the causative virus by Chinese authorities on 7 January [1]. Our normal life is on hold due to the escalating novel coronavirus COVID-19 emergency. The ongoing spread of the COVID-19 pandemic is having profound effects on our private and professional life, the world economy, and the social organization of the communities we live in [2]. The recent emergence of this virus, which is causing an outbreak of unusual viral pneumonia in patients and different animals which will help to understand the biology and potential risk of coronavirus that exist in richness in wildlife such as bats [3]. The novel coronavirus SARS-CoV-2 that causes COVID-19 is now likely to become the fifth endemic coronavirus in the human population. Recently, scientists are working to decipher its genome to help us stop other coronaviruses from entering the human population. They don't know "the procedure of transfer of the novel coronavirus which causes COVID-19 made the leap from animals to humans" is a puzzle and scientists are trying to solve as humanity comes to grip with the deadly pandemic sweeping the globe and it is concluded that "coronaviruses can jump species boundaries and adapt to new hosts, making it straightforward to predict for the more emergence in the future." How we respond to that will require more research to assist develop public health policy. They point to policy and other measures to help prevent other coronaviruses from becoming a health danger to humans [4]. It is reported that wildlife contains many coronaviruses that could potentially emerge in humans in the future. A very important lesson from this pandemic to help prevent the next one is that humans must not expose them to wildlife, for example by banning 'wet markets' and the trade in wildlife and the pangolins play a vital role in the emergence of SARS-CoV-2 which causes the COVID-19, is still unknown. It is also interesting that the pangolin viruses contain some genomic regions that are very closely related to the human virus and the receptor-binding domain is the most important which dictates how the virus can attach and infect human cells " -said by Professor Holmes [5]. A new coronavirus (CoV) identified as the COVID-19 virus is the etiological agent responsible for the 2019-2020 viral pneumonia outbreak that commenced in Wuhan [6], [7], [8], [9]. For this, WHO responds to the outbreak, the Research and Development Blueprint has been activated to accelerate diagnostics, vaccines, and therapeutics for this novel coronavirus [1]. Whenever turn on television news, some expert is saying "There is currently no effective treatment for coronavirus." Currently, there is no targeted therapeutics and effective treatment options remain very limited which plays a pivotal role in mediating viral replication and transcription, making it an attractive drug target for this virus [10], [11]. It is reported in a chapter entitled "Genetic Resistance to Coronavirus Infection-A Review" [12]. In another study, it is reported that 2019-nCoV is sufficiently divergent from SARS-CoV to be considered a new human-infecting betacoronavirus, and our phylogenetic analysis suggests that bats might be the main host of this virus, an animal which is used at the seafood market in Wuhan might represent an intermediate host facilitating the emergence of the virus in humans. Especially, structural analysis suggests that 2019-nCoV might be able to bind to the angiotensin-converting enzyme 2 receptor in humans. The future evolution, adaptation, and spread of this virus warrant urgent investigation [13]. Recently, in 'India Today' discuss the "Trade in the time of COVID-19: The economic impact of coronavirus on India and beyond" shows the estimates of India's aggressive 21-day lockdown could bring the country's growth down to 2.5 % from the 4.5 %. The effect of coronavirus is likely to be seen long after medical science offers a cure or at least a vaccine [14], [15]. In a joint report from the World Health Organization (WHO) and the World Bank estimates the impact of such a pandemic at 2.2 percent to 4.8 percent of global GDP (US$3 trillion) [14], [15]. That was well before the world knew of Covid-19. So, the effect of the virus is likely to be seen long after medical science offers a cure or at least a vaccine for Covid-19 (the disease caused by the new coronavirus). That's because the economic cost of shutting down large swathes of the world is going to be steep [14], [15].
It is reported that the novel coronavirus infection became a pandemic because it thrives on asymptomatic patients of Covid-19 and different health agencies the world over has been firm in dealing with symptomatic Covid-19 patients. It will be faced when the real challenge has been to identify those Covid-19 patients who don't show any symptoms which have forced affected countries to revise their death toll because novel coronavirus is still an unknown enemy to humans. It may be possible for the symptoms which are currently atypical for Covid-19 may evolve into key indicators of novel coronavirus infection in months and years to come because, as the World Health Organization warns, this virus is going to stay among humans for a long time [16]. In theory, challenge trials could enroll volunteers who are at low risk of harm from the virus that causes COVID-19-dubbed SARS-CoV-2-such as young adults who rarely develop serious symptoms after becoming infected naturally. "Now this pandemic situation is analogous to war, in which there is a long tradition of volunteers risking their health and lives on dangerous missions for which they know about the risks and are willing to do so to help save the lives of others," the letter states. "The delay of every week in the deployment of a vaccine to the seven billion humans on earth will cost thousands of lives." Foster counters that testing a vaccine in health care workers who are needed in a hot spot also has risks, as it might take them out of work for a time. He hopes the letter "just makes the FDA a little less scared," stressing that "it's pretty clear that the only way we're really finally going to get out of this (pandemic) is when we have an effective vaccine" [17]. Antimalarial drugs widely used against COVID-19 heighten the risk of cardiac arrest. How can doctors minimize the danger? [18].
In the Drug Target Review's hub for COVID-19 research hub, reported that no vaccine currently available for this coronavirus which has spread swiftly across the globe, resulting in devastating effects on the world's economy and an ever-increasing number of fatalities. To support collaboration in this field, they have gathered the latest news and updates relating to COVID-19 drug discovery efforts [19].
More than 100 treatments and vaccines are in development to stem the COVID-19 pandemic, and some onlookers have worried that this sprawling and potentially duplicative effort is wasting time and resources. Hoping to bring order to the chaos, the National Institutes of Health (NIH) and major drug companies today announced a plan to stage carefully designed clinical trials of the drugs and vaccines they have decided are the highest priorities for testing and development [20]. On 30 January 2020, Global research and innovation forum: towards a research roadmap; following the recommendations of the Emergency Committee, the Director-General of WHO declared that the outbreak constitutes a Public Health Emergency of International Concern (PHEIC). The world scientists on COVID-19 met at the WHO's Geneva headquarters from 11 to 12 February 2020 to assess the current level of knowledge regarding the new virus, agree on critical research questions that need to be answered immediately, and ways to work together to accelerate and fund priority research that can contribute to curtail this outbreak and prepare for the future outbreaks [1].
To move forward, it will require new and more efficient solutions, science and technology applications, products, and it has to fulfill all requirements. To meet the challenge of the problems; it is reported that the clinical homeopathic biomedicines; Aakashmoni or Acaciasides (A&B) or Acacia auriculiformis -extract, prepared from the funicles or fruits of Acacia auriculiformis A. Cunn. (Plate 1), is being used traditionally to overcome various medical complications and it has been used to treat several medical ailments due to its low toxicity and high efficacy and presence of effective bioactive phytoconstituents of this biomedicine and more than thirty-five diseases are effectively controlled with these biomedicines by inducing their natural immunity in animals [21], [22] and it is highly effective in ameliorating different diseases like root-knot, leaf spot, powdery mildew, mosaic and tukra diseases in different plants and root callous by using their defense-response against pathogen infection and leaving no residual toxicity in the leaves to affect the growth [22], [23], [24], [25], [26], [27], [28], [29], [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40], [41], [42], [43], [44], [45], [46]. Recently, I published a paper entitled "Enriched Science and Technology Communication Economy in Agriculture by Use of Acaciasides as Potential Bio-Agents Against Various Pathogens" [35], [47]. Biomedicines (any phytomedicines-or agents-of biological origin derived from plants source) provide a new class of biological compounds that stand as a suitable and useful alternative to conventional but hazardous methods of chemical control against pathogens [22], [25], [35].
Our main aim was to further investigate new and more efficient solutions, technologies, and products for controlling disease, by using ultra-high diluted homeopathic biomedicine-Aakashmoni with pretreatment, prepared from the fruits of Acacia auriculiformis A. Cunn, mixed with water at an extremely low dose, was applied by foliar spray once daily for 15 days @ 10 ml/plant, against naturally occurring, root-knot-, leaf spot-, powdery mildew-, mosaic-and tukra-diseases of mulberry (Morus alba L., cv. S1), in a field trials and silkworms rearing, by inducing their natural defense response for preventing diseases and resource productive economies applications. The main purpose of the present investigation is to confirm the efficacy of pretreatment effects with the Aakashmoni at an extremely low dose, against various mulberry plants-pathogens.
Recently there is no targeted therapeutics and effective treatment options remain very limited. So, the effect of the virus is likely to be seen long after medical science offers a cure or at least a vaccine for COVID-19. Now it is planned to publish four confirmed-suggestions for current outcomes and therapies on coronavirus disease (COVID-19) outbreak that helps the readers as well as a scientific community to take measures or treatment opportunities or discover the best vaccines to avoid new coronavirus. Our main goal is to limit infections. To overcome the situation, the confirmed-suggestion for clinical study may be arranged in near future or as early as possible by the researcher for the discovery of the best 'Vaccine' or at least treatment by using ultra-high diluted homeopathic biomedicines-Aakashmoni 30C at an extremely low dose, which may kill the COVID-19 by boosting our immune system which may significantly solve or help or advance the current problem of the COVID-19 infections and clinical practice and acts as an indispensable source to access the pharmacological developments globally.

PREPARATION OF POTENTIZED LIQUID AAKASHMONI 30C MEDICINE
• The homeopathic mother tincture of A. auriculiformis, named Aakashmoni MT were diluted with 90% ethanol (1:100) proportionate in a round vial. • The vial was filled up to two-thirds of its space, tightly corked.
• And then were given 10 powerful downward strokes of the arm.

PREPARATION OF MEDICATED AAKASHMONI 30C GLOBULES
• Aakashmoni 30C homeopathic potencies in liquid form can be kept in globules. A vial was filled up to two-thirds of its empty space with sucrose globules of a particular size. • Few drops of a liquid potency of homeopathic biomedicine-Aakashmoni 30C were poured into the vial to just moisten all the globules. • The vial was corked and then shaken so that all globules were uniformly moistened.
• The cork was loosened and the vial was turned upside down to allow excess liquid drain out.

PREPARATION OF AAKASHMONI 30C CONTROL GLOBULES
• A vial was filled up to two-thirds of its empty space with sucrose globules of a particular size.
• Few drops of 90% ethanol were poured into the vial to just moisten all the globules.
• The vial was corked and then shaken so that all globules were uniformly moistened.
• The cork was loosened and the vial is turned upside down to allow excess liquid to drain out.
• After keeping the vial in the inverted position for nine to ten hours, the vial was turned upright, well corked, and kept in a cool dry place away from light. • The dry globules were then kept in a vial to retain their properties for many years.

MORTALITY TEST
Two sets of cavity blocks with 1 ml distilled water containing 50 larvae (J2) of M. incognita were taken; one set was treated as control and the other was treated as treatment set. To assess the direct effect of homeopathic medicine-Aakashmoni 30C pretreatment -test solution, the water was removed by pipette from all the treatment sets, and immediately replaced by 1 ml of pretreatment -test solutions of Aakashmoni 30C (7.2 mg globules/ml concentration) were added respectively. To assess the direct effect of the control solution, the control set was received 1 ml of control solution and observed with every 30 minutes interval for a period of 12 hours exposure period at room temperature (25 ± 2°C). This mortality test was replicated five times. It was noted that both the control (without Aakashmoni) and treatment (with Aakashmoni) set were received sucrose globules. This mortality test was replicated five times [28], [32], [36], [40], [41], [42], [43], [44], [45], [46], [47], [48], [49], [50], [51], [52], [53], [54].

SITE OF THE PRETREATMENT EXPERIMENTAL PLOTS
The field experiment (Plate 2) was carried out at the Sriniketan Sericultural Composite Unit, Government of West Bengal, India where the temperature was 28 + 5°C and relative humidity was 75 + 5 % (Plate 2) [11], [12]. Throughout the year, the whole mulberry field was naturally infected with root-knot disease of mulberry plants (Morus alba L., cv. S1) caused by Meloidogyne incognita (Kofoid & White) Chitwood root-knot nematodes pathogens.

PLANTATION OF MULBERRY CUTTING
Mature three years old mulberry cutting, Morus alba L., cv. S1 (average 25cm length and 20g fresh weight) collected from the same sericulture field, were planted with a gap of 45cm throughout the experimental fields where there were no soil differences and climatic conditions. The planted mulberry cuttings were allowed to grow for a period of three months. Regular rhizospheric soil and root sampling (at random) were done for estimation of the nematode population during this three month growth period of mulberry in all fields [25], [26], [27], [28], [29], [32], [35], [50], [51]. At least 80 number at random rhizospheric soil sampling (200g in each sample) were collected from rhizospheric root-soil area of root (10-15cm X 10-15cm) and at least 40 number at random root sampling (2g fresh root in each sample) were collected from newly formed roots (or gall roots) for determining the intensity or presence of nematodes in all the pretreatment experimental fields.

DIVISION OF GROUPS AND PLOTS
After three months of growth of mulberry, the M. incognita population was estimated in the rhizospheric soil as well as roots fields [25], [26], [27], [28], [29], [32], [35], [50], [51] (at least 40 at random sampling in each area) of mulberry plants in each area of the mulberry field. The M. incognita infected mulberry plants were achieved growth of 50-60 cm in height. The infected mulberry plants were divided into 16 plots (Plate 2), each measuring the area of 472.44cm X 533.4cm X 45.72cm. The mulberry plants divided into two plant groups; untreated plant groups and pretreated plant groups and each group has 8-plots (20plants/plot). At first all the plants were pruned, manured with NPK, and irrigated every 7 days. Rhizospheric soil was interchanged among the plants to keep the nematode infestation as uniform as possible in the naturally infected field. After pruning, the plants were allowed to grow for a period of 137 days when their root-knot, leaf spot, powdery mildew, viral and tukra diseases were assessed [32], [33], [38], [39], [56], [57]. The field trial was replicated three times.

ANALYSIS OF RESIDUE
A thin layer chromatography plate (TLC) was made with silica gel. Mulberry leaves, collected one day after the last pretreatment were homogenized in a blender and extracted with ethanol. The residue was applied at one end of the plate as a small circular spot. The initial spot should be compact for reproducible Rf-values and zones should Homeopathic Medicines-Aakashmoni Will Be the Best Vaccine Against Covid-19: Enriching Agriculture Science and Technology Communication Mechanism Application Issues! always be placed at the same distance from the surface of the developer. Here, the residues run in thin layer chromatography plate (TLC) with the standard from the homeopathic medicine-Aakashmoni 30C -pretreatment test substances [22], [25][26], [27], [28][30], [31], [32], [35], [36], [46].

SCIENCE TECHNOLOGY COMMUNICATION APPLICATIONS
The activity of students, researchers, regulators, teachers, staff, community, photographers, visitors, different scientist, academicians, clinicians, administrators, institutions, farmers, NGO, and media personnel, -campaign oraware or -make the news or -publication regarding the importance of "Pretreatment with Ultra High Diluted homeopathic Aakashmoni 30C, at an Extremely Low Doses, Use as Potential Cost-Effective Biomedicine Against Various Pathogens: Enriching Science and Technology Communication Applications Economy and Healthcare-, Defense response-and Immunity-as well as Biodiversity Conservation-Issues" in different audiovisual media (TV channels), social media, web pages, newspapers, and journals are recorded. It is a platform to promote and discuss different new issues and developments by publishing case reports in all aspects of Clinical Homeopathic Medicine for all over the globe [22], [25] [26], [27], [28], [30], [31], [33], [34] [35], [59], [63], [64], [64], [65].

FUTURE SUGGESTIONS IN RESEARCH
It will be achieved from typical analysis or justifications of literature review, research articles, specifies hypotheses, backgrounds, problems, brief review of the key literature, reports of clinical research trials or fields, note of any relevant controversies or disagreements in the trials or field, important references and data or conclusions from the work, extensive discussion of relevant literature as well as present investigation results. Especially, emphasize the new and important aspects of the study and the conclusions that follow from them. For experimental studies it is useful to clarify the main findings, then explore possible mechanisms or explanations for these findings, compare and contrast how the research is different from previous reporting and how the observations will significantly advancement of the current problem or knowledge of the subject, state the limitations of the study. Emphasis of claiming priority of work that has not been completed. Then new hypotheses will arise and clearly label them as such trials for education and prevention are the ultimate keys to extending good health globally [22], [25] [26], [27], [28], [30], [31], [33], [34], [35], [36], [37], [40], [41], [42], [43], [45], [46], [47], [52], [65].

TOXICITY TEST ON MORTALITY
It was observed that homeopathic biomedicines-Aakashmoni 30C had no direct toxic effects on nematodes mortality within the exposure period of 12 hours at room temperature (25 ± 2°C). For this reason, no data were presented in the results section.

ANALYSIS OF RESIDUES TOXICITY
Mulberry leaves collected fifteen days after the last pretreatment, did not contain any toxic residue of the homeopathic medicines-Aakashmoni 30C -pretreated test substance by thin-layer chromatography plate (TLC). Table 1: shows the pretreatment effects of homeopathic medicine-Aakashmoni 30C on M. incognita pathogens infected mulberry plants in a field trial replicated thrice (P<0.01 by 't'-test). All naturally infected plants (Pretreated plant group) pretreated with Aakashmoni 30C showed increase number and surface area of leaves, and higher protein content in leaves and root than infected untreated (control) plants (untreated plant group). In all infected Aakashmoni 30C -pretreated plants, the population of root-knot nematodes decreased significantly in rhizospheric soil and as well as in roots than infected untreated (control) plants. The number of root galls also decreased significantly after Aakashmoni 30C-pretreatment. x,y-Significant difference by 't'-test (P<0.01) in the same row between day-0 and day-30 of each character.    Table 3: shows the pretreatment effects of homeopathic Aakashmoni 30C on diseased infected mulberry plants in a silkworm rearing and field trial replicated thrice on the feeding, growth, and mortality of silkworms (P<0.01 by 't'-test). The average consumption of leaves by the 5th instars (Plate 6), the average number of feeding to cocoon formation (Plate 7), the average number of feeding day to cocoon formation, the average number of escapingfeeding during molting and average molting span days were less for Aakashmoni 30C -pretreated plants than for infected untreated (control) ones. The average mortality rate percent (%) was nil with Aakashmoni 30C -pretreated plant groups and 56% with infected untreated (control) one. However, the average fresh weight of the 5th instars larvae was higher with Aakashmoni 30C -pretreated plants than with infected untreated (control) one.  Table 4: shows the effects of feeding Aakashmoni 30C -pretreated mulberry leaves on silk production, spinning characters, and rearing practices in a silkworm rearing (Plate 7) and field trial replicated thrice (P<0.01 by 't'-test). The average fresh silk gland weight, average fresh cocoon weight, average fresh shell weight, and average shell ratio (SR %) were higher with Aakashmoni 30C -pretreated plants than with infected untreated (control) one. Notably, average starting time to spinning day and average span of spinning day (i.e. duration of span) were fewer with the Aakashmoni 30C -pretreated than with infected untreated (control) ones. The average effective rate of rearing (ERR%), average sex ratio percentage, and average egg-laying capacity were significantly higher with all Aakashmoni 30C -pretreated groups.

FUTURE SUGGESTIONS IN RESEARCH
The results fulfill the goal of a research proposal because the present Pretreatments with homeopathic biomedicines-Aakashmoni 30C need to justify future research and to present the practical ways in which the proposed study should be conducted by the future researcher for conducting the research consistent with requirements of the professional or academic field and a statement on anticipated outcomes and or benefits derived from the study's completion.

SCIENCE TECHNOLOGY COMMUNICATION APPLICATIONS
The students, researchers, teachers, staff, community, photographers, visitors, different scientist, administrators, institutions, farmers, NGOs, and media personnel campaign, aware, discuss, arrange workshops and seminars, make news and publish as abstract regarding the importance of "Pretreatment with Homeopathic Medicine-Aakashmoni 30C, use as Against Various Plant Pathogens: Enriching Science and Technology Communication Applications Economy in Agriculture-, and Healthcare-, Defense Response-, Vaccinations-and Immunity-as well as Biodiversity Conservation-Issues" in different national-and local-audiovisual media (TV channels), different social media, web pages, newspapers and different -national and -international Journals as well as Congress Proceedings also.
For this, the effective rate of rearing (ERR %) is very high in all Aakashmoni 30C -pretreated treatment batches which enriches the sericulture industry in many ways, especially for commercial purposes, and which increased agriculture, economies, social structures, and ecosystems and may hold lessons for a future of increasing climate stability.

AAKASHMONI 30C SYNTHESIS VARIOUS ANTIGENS FOR DEFENSE
It is reported that a plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion [74]. Functional analysis has confirmed a role for this transporter in disease resistance [75]. Though, M. incognita is known to share common antigens with its host plants [76]. It appeared that during natural infection with the nematode, host plants showed minimal defense responses to the nematode because of this antigenic similarity [75]. It is reported that the pure compounds acaciasides (A&B) contain two triterpenoids saponins [25], [32], [35], [48], [54] and these saponins provide defense to the test plants against pathogens [77], [78], [79], [80], [81]. Aakashmoni 30C must be responsible for the defense resistance of the mulberry. The ultrahigh diluted drug -Aakashmoni 30C may synthesis various antigens particularly (low molecular weight proteins; 5kd to 25kd) and induce defense responses involving several pathogenesis-related proteins in which the naturally infected plant pathogens fail to tolerate [25], [32] [35], [48], [53], [54]. It is observed that in lady's finger plants treated with NE (nematode extract) i.e. pretreated showed the highest number of root proteins (no. 24) but in inoculated untreated root was 18 number and uninoculated untreated root was 11 number [25], [32], [35], [37]. Those showed that NE served as a stimulus for the expression of many proteins particularly the defense-related proteins which later provide resistance to pathogen-nematodes infection. However, in the test plant were treated with NE after inoculation i.e. post-treated with live nematodes did not show much increase in the number of proteins (23) in root [25], [32], [35], [37].

AAKASHMONI 30C EXPRESSION OF SOME DEFENSE GENE
Those showed that nematode pathogens infestation somehow serve as a repressor for the expression of defense gene in plant [25], [28], [32], [35], [36], [37], [40], [41], [42], [43], [45], [46]. From this point of view, it can be assumed that ultrahigh diluted pretreated-homeopathic biomedical drug -Aakashmoni 30C at an extremely low dose, serve as a stimulus for the expression of many new induced defense-related PR-proteins by systemic acquired resistance which provides defense-resistance to various pathogens causing major diseases of plants. It can be told that Aakashmoni 30C acquiring systemic resistance could serve very effective eco-friendly phytomedicine and promoted growth of test plants by inducing their defense responses of the host plants by expression of some new proteins against many plant pathogens infection causing major diseases and this ultrahigh diluted pretreated homeopathicbiomedical drug conserved our biodiversity and makes pollution free environment.

AAKASHMONI AS UNIVERSAL POTENTIAL PHYTOMEDICINES
Now the key question is, whether plant-derived natural products, ultra-high diluted pretreated-homeopathic drug -Aakashmoni 30C at an extremely low dose, can be used as potential biomedical by inducing defense-response against various plant pathogens causing major mulberry diseases in a field trial and silkworms rearing. It is surprising that all naturally infected Aakashmoni 30C -pretreated plants not only are less affected by pathogens but also have a better growth than infected untreated (control) plants. The positive effects of growth may be responsible for defense resistance against pathogens. Aakashmoni 30C might have induced synthesis of many new proteins which have stimulated increase photosynthesis rate, stomata-activity and water retention capacity of Aakashmoni 30C -pretreated plants [28], [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40], [41], [42], [43], [45], [46], [82]. The positive effects of growth on disease-infected pretreated plants might not only be responsible for defense resistance to pathogens but also improved growth of silkworm larvae and silk gland weight, cocoon weight, shell weight, effective rate of rearing (ERR%), sex ratio percentage and egg laying capacity of mother moth with zero mortality rate were higher with all Aakashmoni 30C -pretreated groups which increase silk production for commercial purpose. It is proved from the results that silk production is higher in all Aakashmoni 30C -pretreated plants than infected untreated treated (control) plants. Now the answer is, ultrahigh diluted pretreated-homeopathic medicine -Aakashmoni 30C at an extremely low dose was not only highly effective in ameliorating different mulberry diseases but also enriched sericulture industry as well as agriculture industry. And it is also noted that pretreatment with, ultrahigh diluted homeopathic medicine-Aakashmoni MT and -Aakashmoni 30C at an extremely low dose, and Acaciasides (A&B) bioagents at low dose, show more or less same results [25], [28], [32], [35], [36], [37], [40], [41], [42], [43], [45], [46].

FUTURE SUGGESTIONS IN AAKASHMONI RESEARCH
• Identical genetic material: We're not completely human, at least when it comes to the genetic material inside our cells. We all may harbor, as many as, one hundred forty-five genes which have jumped from bacteria, other unicellular organisms, and viruses and made themselves at home in the human genome. All, the scientists indicated hundreds of genes that appeared to have been transferred from bacteria, archaea, fungi, other microorganisms, and plants to animals, they report online today in 'Genome Biology'. In the case of humans, they found one hundred forty-five genes that seemed to have jumped from simpler organisms, including 17 that had been reported in the past as possible horizontal gene transfers [83]. The genomic sequencing showed that this pathogenic coronavirus is 96.2% identical to a bat coronavirus and shares a 79.5% sequence identity to SARS-CoV [84], [85], [86] • Structure and function of genetic material: The genomics of plant and animal is a vast area of research for the biological issues covered because it continues to deal with the structure and function of genetic material underpinning all organisms [87]. Approximately, ten percent of the human genome is made of bits of virus-DNA. Mostly, this viral DNA is not always harmful. In some cases, researchers have found that actually it has a beneficial impact. When viruses infect us, they can embed small chunks of their genetic material in our DNA [88]. The viral content of human genomes is more variable beyond our imagination. Millions of years ago, into the primordial genetic material of our progenitors, parts of human DNA are of viral origin were inserted and have been inherited by successive generations. Thus, the genomes of modern humans are not thought to vary much. • Common virus-derived sequences: HERV (Human endogenous retroviruses) are by far the most common virus-derived sequences in the human genome and mobile DNA shows a mechanism that has introduced more inter-individual variation in HERV content between humans than previously appreciated [89]. It is known that the "Human Genome Is Full of Viruses and Our body requires viruses, but viruses don't always require a body" [90]. • Structure-based design of antiviral drug: It is reported in a chapter entitled "Genetic Resistance to Coronavirus Infection-A Review" where three host resistance mechanisms: genetic control at the level of the, -cellular receptors, -macrophage and -acquired immunity. However, they would like to stress that those 'levels' are purely operational boundaries. In reality, a host can be infected with a virus several times during its lifetime, and thus all available innate and immune resistance mechanisms will be called into play at once. Also, they have included a general outline of the methods used to identify host resistance genes in mouse models of infection [12]. SARS-CoV-2 is the etiological agent responsible for the pandemic COVID-19 outbreak and the main protease (Mpro) of SARS-CoV-2 is a key enzyme that plays an important role in helping in viral replication and transcription. Structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease [91]. • Immune system creates a blueprint: Once the virus infects the host cell, it takes over the host cell's machinery to produce more viruses. The host cell essentially becomes a virus factory. When the human body is attacked by germs, the immune system kicks into gear to fight off the assault. Germ fighting white blood cells in the body are called up to destroy the intruder. These cells target specific sites on the virus, working to destroy the infection. Also, a healthy person's immune system creates a blueprint of the attacking agent. With this blueprint, the body effectively remembers the germ -enabling a person to fight for reinfection by the same or similar viruses [92]. • Use as traditional medicine: In the evolution of human history shows the evidence that people is using traditional medicine for therapeutic purpose. The reports from the World Health Organization (WHO) claim that 70%-80% population is primarily dependent on animals and plant-based medicines because of limited or no access to medical services. The drugs obtained from wild plants and animals are not only used as traditional medicines but also as raw materials in the formulation of modern allopathic and herbal preparations [1], [14]. It is reported that as an internal treatment, the innate response of the patient's immune system to the presence of an invading microorganism has been studied, highlighting anti-microbial peptides as the host's own defense molecules. This work shows a compilation of the most relevant and current antimicrobial peptides that could be used as potential therapeutic agents against microorganisms located in the skin and related to acne disease [93]. But in a case report of a congenital immune deficiency disease -WHIM syndrome is a rare primary immunodeficiency disorder and it is an acronym for some of the characteristic symptoms of the disorder; warts, hypogammaglobulinemia, infections, and myelokathexis. This case report provides data of a patient with recurrent respiratory and cutaneous infection who was diagnosed with WHIM syndrome was presented with chronic productive cough, fever, pleuritic chest pain, chills and sweating also [94]. • Low toxicity with high efficacy of the of major bioactive phytoconstituents: It is reported that the acaciasides (A&B) from fruits of A. auriculiformis, is being used traditionally to overcome various medical complications like sore eyes, aches, rheumatism, allergy, itching, and rashes. Besides, it has also been proven for many pharmacological activities like central nervous system depressant activity, antioxidant, antimicrobial, antimalarial, antifilarial, anticestocidal, antimutagenic, chemopreventive, spermicidal, wound healing, hepatoprotective and antidiabetic activity due to its low toxicity (LD50 = 3741.7 mg/kg) and high efficacy and the various phytochemical investigations reveal the presence of chief constituents as flavonoids and triterpenoid saponin glycosides. The low toxicity and the presence of major bioactive phytoconstituents like flavonoids and triterpenoid saponin glycosides are responsible for a therapeutic remedy for various diseases and pharmacological activities respectively. It has been used to treat several medical ailments due to its low toxicity and the presence of bioactive phytoconstituents [95]. Isolated saponins (acaciasides -A&-B acylated triterpenoid bisglycoside) were screened for their antifilarial activity and results were found to be significant [96]. A US-patent claimed the potential of acaciasides (A&B) isolated from A. auriculiformis for the prevention of HIV infection and as a vaginal contraceptive [97]. • Human trial with saponin-based vaccine: Recently, in the Drug Target Review 2020, NovavaxInc, which contributed to the development of other epidemic vaccines, has announced it is currently in pre-clinical animal trials for several multiple nanoparticle COVID-19 vaccine candidates. The biotechnology company has announced its efforts to help in creating a vaccine against SARS-CoV-2. The company stated they have used their recombinant protein nanoparticle technology platform to generate antigens derived from the coronavirus spike protein and their previous experience working with other coronaviruses, including both MERS and SARS, allowed them to mobilize quickly against COVID-19 and successfully complete the critical preliminary steps to engineer viable vaccine candidates, as said by Stanley Erck, President and Chief Executive Officer of Novavax which adjuvant is saponin-based and it has shown a "potent and well-tolerated effect" [98], [99]. • Successful vaccination: It requires four components; knowing the vaccine target, what kind of immune response, how to generate that response, and understanding responses in the people who we want to vaccinate. Human Immunomics Initiative (HII) aims to decode the underlying mechanisms and rules of how the human immune system fights disease with advances in computing and artificial intelligence, genomics, systems biology, and bioinformatics [100]. And should follow the guideline of WHO entitled "Vaccine-preventable diseases and vaccines" [101]. It is reported that long-stay stress in emergencies can be responsible for this condition in a case study of Tako-Tsubo cardiomyopathy disease which shows that, that's why it's so important to Reduce Wait Time in the emergency as much as possible [102]. SARS-CoV-2, the coronavirus that causes Covid-19, enters human cells by binding of its viral spike protein to the membrane-bound form of them aminopeptidase angiotensin-converting enzyme 2(ACE2) [103]. Studies in animals have suggested that angiotensin-converting-enzyme (ACE) inhibitors and angiotensin-receptor blockers (ARBs) may up-regulate ACE2 expression [104], thus increasing the availability of target molecules for SARS-CoV-2. Ultimately, one or more randomized trials will be needed to answer definitively the question of whether ACE inhibitors or ARBs pose harm to patients with Covid-19 [105]. • Fulfill the target: Here, the results and discussion fulfill the goal for the research suggestions because the present pretreatments with ultra-high diluted homeopathic medicines -Aakashmoni MT or -Aakashmoni 30C liquid or -Aakashmoni 30C globules, prepared from the fruits of A. auriculiformis, mixed with water at an extremely low dose, need to justify future research and to present the practical methods in which the proposed study should be conducted. The plans for conducting research are governed by standards of the results in which the solutions or problems resides, therefore, the guidelines for research proposals are more exacting and less formal than a general project proposal [28], [32], [35], [36], [40], [41] [42], [43], [45], [46], [47].

NOW FIRST SUGGESTION
In homeopathic biomedicines, Aaakashmoni MT (original solution or crude extract i.e., Mother Tincture) may be used in vaccine formulations to regulate immune function by acting as -antioxidants and -scavenge oxidative stress. Because, it is already well-known that the presence of chief constituents as flavonoids and two acylated triterpenoid bisglycoside saponins present in pure compound-acaciasides (A&B) isolated from the crude extract of A.auriculiformis fruits [14], [22], [25], [37], [39], [41], [48][53], [77], [99], [106], [107], [108], [109], [110], [111], [112], [ 113].So, the adjuvant Aaakashmoni MT may be used with recombinant protein nanoparticle antigens derived from the coronavirus spike protein and combine these antigens with its adjuvant Aaakashmoni MT for the final formulation of the vaccine and it may be shown a "potent and well-tolerated effect" through stimulating the entry of antigen-presenting cells into the injection site and enhancing antigen presentation in local lymph nodes, boosting immune responses [32], [35], [98], [99]. In a letter as an e-mail, the Science Advisory Board Net, at Express Cells, for their business of creating better knock-in cell lines for drug discovery, toxicology, and other biologic research and add for purchase SARS-CoV-2 Spike Protein (NC_045512.  [112] and the readily available coronavirus spike proteins may be helped to use for vaccine preparation which may fight against "COVID Toes among kids: New symptom of novel coronavirus infection" [113]. Here, vaccination or treatments, is the use of remedies against diseases either earlier in an epidemic or given routinely to prevent diseases. When the latter is used it involves mostly the users just like any conventional vaccination which administers the antigen in an inactive state to gain immunity towards the disease and is given before the onset of disease or disease symptoms in an individual as a prevention rather than cure [114] and in a clinical study shows the efficiency of triple antibiotic mixture and propolis as intracanal medication in revascularization process in immature apex [111], [114], [115]. It is obligatory that information on ClinicalTrials.gov, a resource provided by the U.S. National Library of Medicine (NLM), to the National Institutes of Health (NIH) or other agencies of the U.S. Federal Government, is provided by study sponsors and investigators, and they are responsible for ensuring that the studies follow all applicable laws and regulations [20], [100], [116]. It is also studied the cost-effectiveness of emergency care interventions in low and middle-income countries like India [117]. But it will not be -cost-effective and not easily -prepare able and -available.

NOW THIRD SUGGESTION
Ultra-high diluted homeopathic medicines -Aakashmoni 30C liquid @ 10 drops mixed with 10 ml of sterile distilled-or pure drinking -water, maybe orally administered once daily (before taking any food) for 15 days, against naturally occurring virus infections15-days before symptom onset OR illness onset (as a vaccine) OR onset of symptoms where patients in hospital-associated COVID-19 infections has been reported (treatments) [119]. In the case of treatment, depending on the disease intensity, the dose may be increased 3-4 times a day. It is moderately cost-effective and easily -prepare able and -available drug [117]. After getting permission from the -WHO, -ClinicalTrials.gov, -U.S. National Library of Medicine (NLM) and -National Institutes of Health (NIH), the Aakashmoni 30C liquid, may be directly used for "Clinical trial or as a homeopathic vaccine" because ultra-high diluted homeopathic medicine -Aakashmoni 30C liquid, have actually no drug molecules (due to cross the Avogadro number i.e., 6.023 X 1023) [20], [100], [116], [120]. It is well known that Avogadro limit washed out by nanoassociates of water which continue as information carriers in serial dilutions and end up with the generalized concept of medicines and these structures, in their turn, influence near-matching bio-molecules to serve as medicines, like antibiotics, leading to a generalized concept of medicine [120], [121].

NOW FORTH SUGGESTION
Ultra-high diluted homeopathic medicine globules -Aakashmoni 30C @ 10 medicated globules (7.2mg), maybe orally administered once daily for 15 days, against naturally occurring virus infections15-days before symptom onset OR illness onset (as a vaccine) OR onset of symptoms where patients admitted in hospital with COVID-19 infections has been reported (treatments) [119]. In the case of treatment, depending on the disease intensity, the dose may also be increased 3-4 times a day. It is the most cost-effective and easy -prepare able andavailable drug [117]. The homeopathic medicine Aakashmoni 30C globules may also be directly used for "Clinical trial or as a Vaccine" after getting permission from the; -WHO, -ClinicalTrials.gov., -U.S. NLM and -NIH [20], [100], [116], [121].

NOW RECENT POSITION OF VACCINES
Recently, scientists in Israel, Netherlands claim progress in COVID-19 monoclonal antibody-47D11 trials 'Such a neutralizing antibody has the potential to alter the course of infection'. But both the treatment -efforts are also in initial stages [122]. In 6th May 2020, E-TOI News focus, "Italy claims to develop first COVID-19 vaccine: Here is the current status of all the potential coronavirus vaccines" [123]. Italian scientists have claimed to develop a vaccine that has successfully generated antibodies in mice that work on human cells [123]. Oxford University initiated a phase-1 human clinical trial of its vaccine on April 23, where two volunteers were injected and Elisa Granato is one of the first ones to get injected with the vaccine. The vaccine -ChAdOx1 nCoV-19-was developed under three months by the University's Jenner Institute. It uses a weakened strain of common cold virus (adenovirus) that causes infections in chimpanzees. For the vaccine to work, scientists have taken the genetic material of the novel coronavirus present on the surface of the virus and put it in the virus [123]. India applied the very old theory for vaccine preparations and plasma therapy is being treated as yet another complementary treatment to help the COVID-19 patients recover [123]. Several hospitals across India have dived in to use plasma therapy for treating the coronavirus patients with the emergency consultancy of Health Minister, Government of India and they apply the HIV-drug combo, has been one of the most touted treatment plans for the COVID-19, which antiviral drug combination was initially found to be effective in treating the coronavirus patients. Prime Minister Narendra Modi reviewed India's status in developing a vaccine for the novel coronavirus, drug discovery, diagnosis, and testing on Tuesday. It was found that as of now more than 30 vaccines are in different stages of development while a few are ready to go to clinical trial stages. It is also important to note that the Pune-based Serum Institute of India is the world's largest vaccine maker and India produces 60 percent of the world's vaccines [123]. The world has joined hands to find a vaccine for the novel coronavirus and scientists and medical researchers across the globe are scrambling for the first breakthrough. Since the virus spreads easily and is already overwhelming the healthcare system of most countries, a vaccine is the most effective way of putting a pause on the spread of infectious disease. At present, almost 80 groups globally are working at break-neck speed for the same, even though a vaccine fit for humans normally takes years to develop. Currently, there are 111 potential vaccines for the SARS-CoV-2 which is in different stages of clinical trials [123].

NOW ADVANTAGE OF AAKASHMONI 30C
While a cure or vaccine for COVID-19 is not available, in the absence of any side-effects and adverse interactions with any conventional medicines along with a robust safety profile and repeated evidence-based successes against viral infections, ultra-high diluted homeopathic biomedicine; -Aakashmoni MT or -Aakashmoni 30C liquid or -Aakashmoni 30C globules, at an extremely low doses, may play an important role in the fight against COVID-19 [119].

NOW PLANNED FOR PUBLICATIONS
Now it is planned to publish four-suggestions for current outcomes and therapies on coronavirus disease (COVID-19) outbreak that helps the readers as well as a scientific community to take measures or treatment opportunity or discovery of vaccine to avoid new coronavirus. Our main goal is to limit infections. Let us all take this basic information's as proposal and also educate people, help them to fight against this war, the normal life of everyone is on hold due to this escalating coronavirus emergency, which in a way helps all the scientist, readers, authors and editors to take necessary and respective steps to save or avoid this dangerous disease. It is requested all to support this initiative and help to reach the targeted audience. At this critical time in the global response to the COVID-19 pandemic, I am proud to be working alongside the scientific community as a coworker that accelerates research, discovery, and testing to contain the SARS-CoV-2 outbreak. And it also focuses the future 'Trends in Medicine Globally' which serves as an evidence-based resource covering various experimental disciplines of medicine, innovative case reports in all clinical practice, and acts as an indispensable source to access the pharmacological developments. And it also deals with articles related to the translational research or investigations in all medical disciplines, epidemiological studies, and general topics of interest to the homeopathic vaccines or social vaccine or COVID-19 epidemic model biomedical research community [124], [125], [126], [127], [128], [129], [130], [131], [132], [133], [134], [135], [136].

NOW THE MOST VITAL QUESTIONN: HOW THE HOMEOPATHIC MEDICINE AAKASHMONI WILL BE THE BEST OR MOST EFFECTIVE AGAINST CORVID-19?
Anyone has been struggled trying to understand the 'idea or message which are trying to communicate is not clear at all' of this paper to avail. It is started by writing about plants that silkworms feed on being helped by Aakashmoni 30c, and then it speculates that the remedy may be boosting the immune system of the plants, and then it leaps to COVID-19. Acacia extract is useful against different plant diseases, but how does that suggest it will be effective against COVID-19? That's the part that is difficult to understand. The following points may be useful as follows: • 1 st cause: It is already reported that the fruits of Acacia auriculiformis, is being used traditionally to overcome various medical complications like sore eyes, aches, rheumatism, allergy, itching, and rashes.

CONCLUSIONS
It can be concluded that the plant-derived natural products, ultra-high-diluted cost-effective pretreatedecofriendly easily available homeopathic drug -Aakashmoni 30C, at an extremely low dose, not only used as a potential biomedical drug by inducing defense-response against various pathogens causing major mulberry diseases, which is sometimes devastating to all kinds of -natural and -artificial vegetation in a field, and silkworms rearing, leaving no residual toxicity in the leaves to affect the growth of silkworms, but also conserve our biodiversity which will contribute towards "Sustainable Climate, Health, and Development". No appropriate antiviral drugs are currently discovered to treat COVID-19. So, to concur the situation, "Four Suggestions for the Best Vaccine and Clinical Trials with Homeopathic Biomedicines-Aakashmoni at an extremely low dose, Against COVID-19 by Boosting Immune System for the Improvement of Science and Technology Communication Applications Economy "Mechanism Issues" forming the 'Best Vaccine', which not only shows the clinical, physical, chemical, biological and physiological effects but also on molecular weight, with mechanism and action of the homeopathic medicines based on scientific experimentations and proofs, and the future clinical-scientists develop all aspects of clinical-case-reports globally by publishing in the Journal with the sincere hope that this crisis will soon be over and the whole world may retain in normal forms by fighting against COVID-19 war.

SOURCES OF FUNDING
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

CONFLICT OF INTEREST
The author has declared that no competing interests exist.

ACKNOWLEDGMENT
The work has been supported by Prof. N.C. Sukul