BIOMEDICINES-CINA AGAINST COVID-19: CONTROLLED PLANT DISEASES ENRICHED SCIENCE AND TECHNOLOGY COMMUNICATION GREEN ECONOMY

The economic-plant-mulberry is infected by different pathogens causing diseases and significantly reduces silk production. Though pesticides are the most effective means of control, but they are expensive and not environment friendly. On the other hand, the ongoing spread of the novel coronavirus, forming epidemic, affecting human civilizations, because recently, no appropriate antiviral drugs for effective treatments or vaccines against COVID-19. To meet the challenge, it is reported that the therapeutic value of traditional-, complementary- and alternative-biomedicines-Artemisia nilagirica, has been used for centuries, to overcome various medical disorders.  So, to concur with the situation, it will require new and more efficient solutions, technologies, and products. Present pretreatment with ultra-high-diluted biomedicines-Cina, prepared from the flowering meristems of Artemisia nilagirica (Clarke) pamp, mixed with water at an extremely low dose, were applied by foliar spray once daily for 15 days @ 10 ml/plant, against naturally occurring, root-knot-, leaf spot-, powdery mildew-, mosaic- and tukra- mulberry diseases, in field trials and silkworms rearing. Pretreatment-investigations have revealed that ultra-high-diluted-Cina, highly effective in ameliorating different mulberry-diseases by inducing their natural defense-response against pathogens-infection and leaving no residual-toxicity in the leaves to affect the growth of silkworms also. It can be concluded that the ultra-high-diluted, eco-friendly easily-available, easily applicable-Cina, at an extremely low dose, not only used as a potential-biomedical-drug against various plant-diseases, by inducing natural-defense- response, but also, it may be effective against COVID-19 for vaccinations and clinical trials by boosting the natural immune system for the improvement of Science and Technology communication cost-effectiveness green-economy and biodiversity conservations as well as bio-applications issues. And the future clinical-scientists develop all aspects of clinical-case-reports by publishing in the appropriate medical research archives, with the sincere hope that this crisis will soon be over and the whole world, may fight against the COVID-19 war, retaining normal forms of human civilizations globally.


TOXICITY TEST ON MORTALITY
The biomedicines; Cina 30C and Cina 200C, at extremely low doses, had no direct toxic effects on nematodes mortality respectively.

ANALYSIS OF RESIDUES TOXICITY
Mulberry leaves collected fifteen days after the last pretreatment, did not contain any toxic residue of the ultrahigh diluted biomedicines; Cina 30C and Cina 200C.

ROOT-KNOT DISEASE
Table1 shows the pretreatment effects of ultra-high diluted biomedicines; Cina 30C and Cina 200C, at an extremely low doses, on M. incognita pathogens infected mulberry plants in a field trial replicated thrice (P<0.01 by 't'-test). Table 2 shows the pretreatment effects of the ultra-high diluted biomedicines; Cina 30C and Cina 200C, at an extremely low doses, on leaf spot, powdery mildew, mosaic viral and tukra diseases of mulberry plants in a field trial replicated thrice assessed initially (Day-0) and after a period of 30 days (Day -30) by 't'-test (P<0.01). Both; Cina 30C and Cina 200C, with pretreatment significantly reduced the number of leaves infected with leaf spot, powdery mildew, mosaic viral, and tukra (Plate 3) as compared to the untreated condition (Day-0). The percentage of control achieved in both drugs respectively; were 60.12%-and 62.08%-for leaf spot, 76.88%-and 77.89%-for powdery mildew (Plate 4), 64.15%-and 64.91%-for mosaic virus (Plate 5), 36.32%-and 38.42%-for tukra infection as compared to the untreated level (Day-0). In the case of infected untreated plots leaf spot, powdery mildew, mosaic viral and tukra diseases showed naturally 27.80 %, 17.76 %, 29.37 %, and 21.20 % reduction respectively, in 30 days (Day -30). Table 3 shows the pretreatment effects of the ultra-high diluted biomedicines; Cina 30C and Cina 200C, at an extremely low doses, on diseased infected mulberry plants in a silkworm rearing and field trial replicated thrice on the feeding, growth, and mortality of silkworms (P<0.01 by 't'-test). The average consumption of leaves by the 5th instars (Plate 6), the average number of feeding to cocoon formation (Plate 7), the average number of feeding day to cocoon formation, the average number of escaping-feeding during molting and average molting span days were less for the both; Cina 30C and Cina 200C, -pretreated plants than for infected-untreated (control) ones. Table 4 shows the effects of feeding the ultra-high diluted biomedicines; Cina 30C and Cina 200C, at an extremely low dose, -pretreated mulberry leaves on silk production, spinning characters, and rearing practices in a silkworm rearing (Plate 7) and field trial replicated thrice (P<0.01 by 't'-test).

FUTURE SUGGESTIONS IN RESEARCH
Here, the results fulfill the goal of a research idea because the present pretreatments with the ultra-high diluted biomedicines; Cina 30C and Cina 200C, at an extremely low dose, need to justify future research.

SCIENCE AND TECHNOLOGY COMMUNICATION GREEN ECONOMY APPLICATIONS
Achieved by; campaign, aware, discuss, arrange workshops and seminars, make news, and publish as abstract regarding the importance of "Pretreatment with the ultra-high diluted; Cina 30C and Cina 200C, at an extremely low doses".

NOW THE KEY ANSWER
Is, the ultra-high-diluted-pretreated-biomedicines; Cina 30C and Cina 200C, at an extremely low dose, was not only highly effective in ameliorating different mulberry diseases but also enriched sericulture industry as well as green agriculture economy. And it is also noted that pretreatment with, the biomedicines; Cina MT and Cina 30C and Cina 200C, at an extremely low dose, and A. nilagirica-extracts or A. auriculifirmis-extracts, at an extremely low dose, show more or less same results [5], [7], [11], [12], [14], [15], [22], [25].

IN GENOME BIOLOGY
We're not completely human, at least when it comes to the genetic material inside our cells; 145-genes from bacteria, other unicellular organisms, and viruses with 17-as possible horizontal gene transfers [84], the genomic sequencing 96.2% identical to a bat coronavirus and shares a 79.5% sequence identity to SARS-CoV [84], [85], [86] , deal with the structure and function of genetic material underpinning all organisms [85], [86], [87], [88]. Approximately, ten percent of the human genome is made of bits of virus-DNA [89]. Human endogenous retroviruses are by far the most common virus-derived sequences in the human genome90 which don't always require a body [91].

IN GENETIC RESISTANCE
It is reported that genetic resistance to coronavirus infection according to those three host resistance mechanisms: genetic control [44] and SARS-CoV-2 is the etiological agent responsible for the pandemic COVID-19 outbreak and the main protease (Mpro) of SARS-CoV-2 is a key enzyme that plays an important role in helping in viral replication and transcription-structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease [92].

IN IMMUNE SYSTEM BLUEPRINT
Once the virus infects the host cell, it takes over the host cell's machinery to produce more viruses. The host cell essentially becomes a virus factory. When the human body is attacked by germs, the immune system kicks into gear to fight, by improving the immune system, creates a blueprint of the attacking agent, by which, the body effectively remembers the germ -enabling a person to fight for re-infection by the same or similar viruses [93].

TRADITIONAL MEDICINE IN HUMAN HISTORY
In the evolution of human history shows the people are using traditional medicine for therapeutic purposes, and the 70%-80% population is primarily dependent on animals and plant-based [37], [46].

THERAPEUTIC VALUE OF TRADITIONAL MEDICINES
It is reported that the 'Indian wormwood'-Artemisia nilagirica, is considered against many ailments as it is possessed to have high content of biologically active molecules and essential oils. It has been used since centuries in antimicrobial, antifungal, antibacterial, and filarial, insecticidal, antiulcer, anticancer, antioxidant, anti-proliferative, healing potential, neurological disorders, tuberous sclerosis, dermal infection, larvicidal, anti-inflammatory activities, anti-asthmatic and anti-malarial activity, Parkinson's disease, Alzheimer's disease, hypertension, diabetes, atherosclerosis, cardiovascular diseases, cytotoxic, malignancy, genetic abnormalities, diabetes, epilepsy, asthma, sychoneurosis, depression, anxiety and stress, leucorrhoea, threatened abortion, hemoptysis, tuberous sclerosis, skin diseases, immunological disorders, and the aging process, and the pharmacological studies confirm its therapeutic value due to the presences of the wide range of chemical compounds which indicate that the plant could Subhas Chandra Datta, Ph.D.
serve as a potent material for the development of novel agents having good efficacy in various disorders in the coming years [54], [55]. It is used for the treatment of human parasites, animals, and plants. The common photochemical of A. nilagirica that causes effective results on human health care biologically active chemical compounds are flavonoids, alkaloids, tannins, glycosides, phenol, saponins, terpenes, amino acids, quinines, phlobatannins and volatile oils, polysaccharides, sesquiterpene lactones, coumarins and acetylenes, and terpenoids [54], [55], [56], [57], [58]. Forty-three constituents (98.16% essential oil); 79.91% monoterpenoids and 18.25% sesquiterpenoids, α-Thujone (36.35%), β-thujone (9.37%), germacrene D (6.32%), 4-terpineol (6.31%), βcaryophyllene (5.43%), camphene (5.47%) and borneol (4.12%), were the major constituents from aerial parts of Artemisia nilagirica [94]. The Artemisia has a diverse range of activities for medicinal uses in human and plant diseases ailments due to possess several active constituents that work through several modes of action and the 1, 8cineole, beta-pinene, thujone, artemisia ketone, camphor, caryophyllene, camphene and germacrene D are the major components in most of the essential oils of this plant species [95]. Artemisia has long been not only used in traditional medicine and but also as a food source for different functions in eastern Asia [96]. Its essential oils has hepatoprotective-and hepatic non-toxic-effects, and as a natural source of hepatoprotective agent96. But, 4th May 2020, WHO recognizes that Artemisia annua are being considered as possible treatments for COVID-19 and should be tested for efficacy and adverse side effects? [97].

THERAPEUTIC APPROACH
Recently, the NovavaxInc, which contributed to the development of other epidemic vaccines, has announced it is currently in pre-clinical animal trials for several multiple nanoparticle COVID-19 vaccine candidates, by using their recombinant protein, the adjuvant is 'saponin-based' and it has shown a "potent and well-tolerated effect" [98], [99]. Scientists are trying to discover novel inhibitor molecules against enzymes Mproand ACE2 by the use of phytochemicals, which be utilized for further innovation and development of antiviral compounds against Coronavirus [100].

VACCINATION
So, for successful vaccination requires four components. Human Immunomics Initiative (HII) aims to decode the underlying mechanisms and rules of how the human immune system fights disease with advances in computing and artificial intelligence, genomics, systems biology, and bioinformatics101. And should follow the guideline of WHO entitled "Vaccine-preventable diseases and vaccines" [37], [101], [102]. It reduces wait time for emergency vaccination [105] and one or more randomized trials will be needed to answer 106 . Then, with allopath is using trial and error method in some cases to treat COVID-19 [97], [107] and according to the World Health Organization's latest table of COVID-19 vaccines, 124-candidates with technologies or platforms. Merck's new investments focus on two different COVID-19 vaccines that are already in early clinical trials [108].

IDEA-I:
So, the adjuvant Cina MT [97], [113] may be used with recombinant protein nanoparticle antigens derived from the coronavirus spike protein and combine these antigens with its adjuvant Cina MT for the final formulation of the vaccine and it may be shown a "potent and well-tolerated effect" through stimulating the entry of antigen-presenting cells into the injection site and enhancing antigen presentation in local lymph nodes, boosting immune responses [5], [7], [11], [12], [14], [15], [16], [18], [22], [25], [33], [60], [98], [99], [114]. In a letter as an e-mail, the Science Advisory Board Net, at Express Cells, for their business of creating better knock-in cell lines for drug discovery, toxicology, and other biologic research and add for purchase SARS-CoV-2 Spike Protein [115] and the readily available coronavirus spike proteins may be helped to use for vaccine preparation which may fight against "COVID Toes among kids [116], which gain immunity before the onset of disease or disease symptoms in an individual as a prevention rather than cure [117] and in a clinical study shows the efficiency of triple antibiotic mixture process in immature apex [117], [118]. It is obligatory that information on ClinicalTrials.gov, a resource provided by the U.S. National Library of Medicine (NLM), to the National Institutes of Health (NIH) or other agencies of the U.S. Federal Government, is provided by study sponsors and investigators, and they are responsible for ensuring that the studies follow all applicable laws and regulations [116], [117], [118], [119], [120], [121]. It is also studied the costeffectiveness of emergency care interventions in low and middle-income countries like India [117], [118], [119], [120]. But it will not also be; -cost-effective and not easily -prepare able, -available and -not help in green economy applications [5], [7], [11], [12], [14], [15], [16], [18], [22], [25], [33], [60], [97], [113].

IDEA-III OR IDEA-IV
The ultra-high diluted biomedicines; -Cina 30C liquid OR -Cina 200C liquid, @ 10 drops mixed with 10 ml of sterile distilled-or pure drinking -water, maybe orally administered once daily (before taking any food) for 15 days, against naturally occurring virus infections15-days before symptom onset OR illness onset (as a vaccine) OR onset of symptoms where patients in hospital-associated COVID-19 infections has been reported (treatments) [123], [124]. In the case of treatment, depending on the disease intensity, the dose may be increased 3-4 times a day. It is moderately cost-effective and easily -prepare able and -available drug [117], [118], [119], [120]. After getting Subhas Chandra Datta, Ph.D.
Recently, scientists in Israel, Netherlands claim progress in COVID-19 monoclonal antibody-47D11 trials [126] and Italy claims to develop first COVID-19 vaccine 127 . India applied the very old theory for vaccine preparations and plasma therapy is being treated as yet another complementary treatment to help the COVID-19 patients recover [127]. The current pandemic locust attack in India causes the crises of food securities [128], the world has joined hands to find a vaccine for the novel coronavirus. At present, many groups (80) are working globally for vaccines for humans which normally takes years to develop. Currently, there are 111 potential vaccines for the SARS-CoV-2 which are in different stages of clinical trials [127].
While a cure or vaccine for COVID-19 is not available, in the absence of any side-effects and adverse interactions with any conventional medicines along with a robust safety profile and repeated evidence-based successes against viral infections, the ultra-high diluted biomedicine; -Cina MT or -Cina 30C liquid or -Cina 30C globules, at an extremely low dose, may play an important role in the fight against COVID-19 123 . It is the most; cost-effective, easily prepare able, easily available, and help in biodiversity conservations and green economy applications issues also [5], [7], [11], [12], [14], [15], [16], [18], [22], [25], [33], [60] and should be applicable for clinical trials as early as possible [97], [113]. Now it is planned to publish six-ideas for current outcomes and therapies on coronavirus disease (COVID-19) outbreak that helps the readers as well as a scientific community to take measures or treatment opportunities or discovery of vaccines to avoid new coronavirus.

Conclusions
It can be concluded that the plant-derived natural products, ultra-high-diluted cost-effective, easily applicable, pretreated-ecofriendly easily available drugs; Cina, at an extremely low dose, used as a potential biomedical drug by inducing defense-response against various pathogens causing major mulberry diseases and the "Six Ideas; for vaccine and clinical trials with the ultra-high diluted biomedicines; Cina MT OR Cina 30C liquid OR Cina 200C liquid OR Cina 30C globules OR Cina 200C globules, at an extremely low dose, against COVID-19 by boosting the immune system for the Improvement of Science and Technology Communication Green Economy Applications Issues" and the future clinical-scientists develop all aspects of clinical-case-reports globally with the sincere hope that this crisis will soon be over and the whole world may retain in normal forms by fighting against COVID-19 war.     a,b-different small letters in a column show significant difference by 't'-test (P<0.01).
* -average values of 180 silk worm larvae in triplicate. + -average values of 10 silk worm larvae and cocoon were dissected in triplicate.

SOURCES OF FUNDING
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

CONFLICT OF INTEREST
The author have declared that no competing interests exist.

ACKNOWLEDGMENT
The work has been supported by Prof. N.C. Sukul  Goal" for arranging to many awareness programmed on COVID-19. Last but not the least, I am thankful to the eminent educationist Sri Tapaprakash Bhattacharya for inspiration and guidance.