THE HUMAN HAIR FOLLICLE PULSATING BIOMAGNETIC FIELD REACH AS MEASURED BY CRYSTALS ACCRETION

This manuscript introduces the biomagnetic fields reach (BMFs) of the human hair follicles. The introduction of a novel table top optical microscopy technique using a special Prussian Blue Stain solution (PBS) mixed with fine iron particles has produced numerous papers confirming the inherent biomagnetism of the human hair. This technique allowed for the design of sets of incremental stacked glass slides for the purpose of measuring the human hair follicle BMFs reach out. This was demonstrated (measured) by using diamagnetic as well as paramagnetic Potassium Ferrocyanide preparations mixed with fine iron particles. Still microphotographs and videorecordings are presented.


Introduction
Ever since the introduction of a simplified method for the detection of electromagnetic energy in plants and animal tissue (1); the intrinsic biomagnetism in humans and rodents hair follicles have been documented (2,3,4,5,6). Of relevancy to this manuscript is a published paper demonstrating the hair follicle biomagnetic fields penetrating through a 25x75x1mm glass slide (7). This finding triggered the idea of measuring the biomagnetic reach (distance) of the human hair follicle. The intrinsic unique pulsating nature of biomagnetic fields emitted by the hair follicle has been previously observed by this researcher. In several occasions, the interaction of the hair BMFs were observed slowing evaporation line progression of the PBS solutions. In addition, precedent exists as to the crystallization of potassium ferricyanide in the presence of magnetic fields (8).

Materials and Methods
Special Prussian Blue Stain solution for this study was supplied by Benjamin Scherlag PhD, Professor of Mredicine, Health Science Center, Oklahoma University, Oklahoma City, USA. The hair samples were self-plucked via tweezers from my scalp or forearms.
A fine iron particle solution was prepared as described in (1) as follows: "by mixing several grams of powdered iron filings (Edmond Scientific, Co., Tonawanda, NY) in 200 cc of deionized water (resistivity, 18.2 MΩ.cm). After standing for several hours the supernatant was carefully decanted for sizing of the very fine iron particles. The particle size and distribution of the particles from the supernatant was determined using dynamic light scattering (DLS) and the zeta potential using phase analysis light scattering by a Zeta potential analyzer (ZetaPALS, Brookhaven Instruments Corp, Holtsville, NY). For sizing, 1.5 ml of the solution in de-ionized water was scanned at 25 °C and the values obtained in nanometers (nm). A similar aliquot of the fine iron particle solution was scanned for 25 runs at 25 °C. for determining zeta potentials. Zeta potential values were displayed as millivolts (mV). A solution having diamagnetic properties was prepared by mixing aliquots of a Potassium Ferrocyanide solution as follows: 2.5% Potassium Ferrocyanide solution (K4Fe2CN6) and a 2.5% HCl. Also added were two parts of the Fe 2000 solution. The composite solution is abbreviated throughout the manuscript as "Fe2." A solution having paramagnetic properties was also prepared by using aliquots of a Potassium Ferricyanide solution as follows: 2.5% Potassium Ferricyanide solution (K4 Fe3 CN6) and a 2.5% HCl, also added were two parts of the Fe 2000 solution. The composite solution is abbreviated throughout the manuscript as Fe3" (1)".

The Stacked Sides Assembly or Sandwich (SDW)
The hair was placed on a 25x75x1mm glass slide, and then covered by an equally sized slide. This preparation is referred in this manuscript as a sandwich (SDW). The Ferrocyanides solutons were used as sentinels in each incremental slide preparation. These solutions will be referred as PBS Fe2 or PBS Fe3 throughout the manuscript. Only one freshly plucked forearm human hair was sequentially used for all three experiments.

The Stacked Slide Preparations
Glass slides were sequentially assembled to increase the vertical distance from the sandwiched hair follicle to the PBS Fe solutions at 1 mm stages ( Figure 1

Hair in SDW at 2 mm vertical distance from the PBS Fe2 drops
The same technique as above was duplicated, except that this time the SDW had two glass slides on the top surface. This made the hair follicle to PBS Fe2 solution at 2 mm.

Hair in SDW at 3 mm vertical distance from the PBS Fe2 drops
The same technique was reproduced, except that this time the SDW had three slides on the top surface. This made the hair follicle to PBS Fe solution distance at 3 mm.
Note: In all three slides preparations the PBS Fe2 were allowed to evaporate. Still images and video-recordings were made by using a Celestron II video-microscope. The images downloaded onto an Apple computer McBook Pro Photo Application.

Results
The hair vertical biomagnetic field range One slide covering the hair (video-recording analysis) As previously described, a freshly plucked forearm hair follicle was placed onto the center of a clean slide. The sticky property of a fresh follicle made it easy for adherence to the glass slide, Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [293] care was taken to maneuver the shaft with a toothpick, and assure that it was also within the slide's boundaries.
Another clean slide was then placed on top of the first and two drops of the PBS Fe2 solution was applied. The thickness of the glass slide measures 1 mm, therefore no contact with the hair below. The solution was allowed to evaporate and continuously monitored. As the evaporation line reached the latitude in front of the root, it stopped and then continues its path (Fig 2) and supplementary video: https://youtu.be/uddsCIeEdMc

Two Slides Covering the Hair SDW
The top slide was removed; care was taken to assert that the hair on the bottom slide remained in position. This time two additional 1 mm slides were added to the SDW. Two drops of the PBS Fe2 solution were also applied. The top slide was allowed to evaporate, this time also the evaporation line stopped in front of the root area for approximately the same time and then continue its path past the bulb and permanently stopped over the bulge area (Fig 3). This time the evaporation showed a latitudinal shift. Now instead of stopping over the bulb area, it was seen over the bulge area. This is attributed to the "Faraday Effect" on EMF vertical propagation. The distance now for the vertical effective magnetic force effect on crystallization was 2 mm. Please note that the thickness of the crystallization line is inversely proportional to the Biomagnetical Field Reach distance

Three Slides Covering Hair
This time the top slide was removed and two clean slides were added; thus making the hair follicle to the top slide at a vertical distance of 3 mm. The evaporation line was unaffected and continued in its path. The distance now from the hair to the top slide surface was 3 mm (Fig 4).

Biomagnetic Field Reach of the Hair Follicle Using a Diamagnetic Solution as Sentinel
Using a tabletop optical microscopy technique; and a Prussian Blue solution tailored to detect biomagnetic fields in plants and animal tissues, the human hair biomagnetical field reach distance was measured. A fresh tweezers plucked in toto (root and shaft) forearm hair was sandwiched (SDW) between two 1 mm thick glass slides. The PBS Fe2 solution was placed on the top slide and allowed to evaporate. Video-recordings of the event were recorded and analyzed. The same procedure was done by preserving the original hair in the SDW and increasing the hair to PBS distance by 1 mm increments.
All recordings showed an effect on the crystallization advance by the biomagnetic field reach of the hair. The effect was not detected at a maximum vertical distance of 3 mm. After analyzing the data, it was concluded that the vertical hair follicle biomagnetic reach using a diamagnetic solution is ≥ 2 ≤ 3 mm.

Conclusions
The hair follicle has been described as a dynamic mini-organ with a variety of different cells interactions and fluctuating metabolic processes (9). Due to the hair follicle intrinsic electron transport based metabolism these biologic entities emit variables or "pulsating" electromagnetic fields. This pulsation is observed when using a paramagnetic solution (Ferricyanide).