VALORIZATION OF SUPERABSORBENT POLYMERS FROM USED DISPOSABLE DIAPERS AS SOIL MOISTURE RETAINER

This study was conducted to explore the potential of superabsorbent polymers (SAPs) from used disposable diapers as soil moisture retainer. Swelling behavior of the proposed hydrogel in response to external stimuli such as salt solutions, temperature and pH was studied. In addition, laboratory experiments were carried out to evaluate the effects of incorporation hydrogel on germination of bean (Phaseolus vulgaris L.) and pumpkin (C. pepo) seeds. The structure of the used superabsorbent was characterized by Fourier transform infrared spectroscopy (FTIR). The results indicate that the proposed SAP exhibited a maximum swelling capacity of 189 g-g -1 of dry gel. It was observed that the swelling capacity decreased with an increase in the ionic strength of the swelling medium. When this SAP was mixed with sandy soil, the mixture was able to lose water more slowly. The seeds germination and seedling growth was remarkably influenced by the application of 0.5, 1.0 and 2.0 w/w% of SAP compared to the untreated soil. Therefore, it follows that it is possible to take advantage of SAPs property from used disposable diapers to retain the moisture in soil as an alternative to value the use of such waste, showing that it has potential for agricultural applications.


Introduction
One of the most alarming problems in the world today is waste management. The generation and disposal of waste is an intrinsic part of any developing society. A major problem in urban areas is the generation of solid waste including used disposable diapers. These items facilitate human life, but their life span is very short and the manufacturers do not anticipate its disposal; they are simply deposited in landfills, causing serious environmental pollution problems (Cordella et al., 2015). Nowadays, it is estimated that 90-95% of diapers used in developed countries are disposable (Kosemund et al., 2009). SEMARNAT (2012a) reported that the total waste generation in Mexico increased from 31.4 million tons in 2001 to 42.1 million tons in 2012, a 25.4% increase in 11 years at a growth rate of 2.3%.
In Mexico the composition of waste generated yearly is mostly organic matter (38%) and potentially recyclable materials (40%) (SEMARNAT, 2012b; Espinosa-Valdemar et al., 2014). According to Espinosa-Valdemar et al. (2011), diapers accounted for 6% of urban solid waste generated in Mexico in 1997, now this amount has increased up to 15%. The typical composition of a disposable baby diaper has been described by EDANA (2008) and Ng et al. (2012): fluff pulp 36.6%, SAP 30.7%, polypropylene 6.2%, low density polyethylene 16%, tape, elastic and adhesive 10.5%. Disposable diapers are often seen as being a major problem, however, they can also be treated as a valuable partially biodegradable resource that can be recovered. Recycling of its organic content is an attractive option both, as a resource and waste management strategy (Sannino et al., 2009). At present, used diapers are generally not collected separately and are disposed of as solid municipal waste for further treatment, mainly by incineration, land filling (Mirabella et al., 2013) and, to a lesser degree, by composting (Colón et al., 2013) or anaerobic digestion (Torrijos et al., 2014).
Waste recycling is an important aspect of environment sustainability, on the other hand, minimizes waste products placed in landfills, promotes recovery of materials that can be reused in new productive cycles, prevents energy usage and consumption of fresh raw materials and allows to preserve natural resources and the environment for future generations. A method has been patented for the separating products such as disposable diapers containing superabsorbent polymers into their constituent parts, including a plastic component, super absorbent polymers, and cellulosic fibers, and further refinement of said constituent parts (Grimes, 2012). Biological treatments that valorize the cellulose content of used diapers, such as composting, and use as substrate in edible mushroom production have been investigated at laboratory (Colón et  Because of this, the need arises to propose strategies to mitigate the accumulation of disposable diapers, through the recovery and use of the superabsorbent polymer in conserving soil moisture. Recently, the diverse applications of superabsorbent polymers are still being expanded to many fields including agriculture and sealing composites, horticulture, drilling fluid additives, artificial snow, medicine, and so on (Rosa and Casquilho, 2012 increasing infiltration and reducing water use and soil erosion (Gao et al., 2013;Essawy et al., 2016). A major advantage of using SAP is its ability to make use of moisture present in soil such as clay, and make it available to the roots. SAP amended soils have better nutrient release, high nitrification, reduced microflora and bacterial content (Demitri et al., 2013;Eneji et al., 2013).
Hence, the aim of this study was to investigate the potential of superabsorbent polymers from used disposable diapers, an abundantly available solid waste, as a nonconventional soil moisture conditioner. The effects of ionic strength, temperature, and pH of the external solution on the swelling capability were investigated. The use of the hydrogel for the cultivation of bean and pumpkin was also investigated at laboratory conditions.

2.1.Hydrogel Recovery
The wet disposable diapers were collected from the local nursery house in Ixtlahuaca -EM, Mexico. Collected disposable diapers only contained liquid waste (i.e. human urine). The diapers were first heat-inactivated by autoclaving at 125 °C for 15 minutes to eliminate pathogens. After sterilizing, the diapers were chopped up and the components were separated in two parts: cellulose and plastic. The superabsorbent polymer particles intermingled with cellulose fibers were oven dried at 60 °C for 24 h. The dried hydrogel was retrieved by gently shaking the cellulose fibers in a plastic container in order to freeing the particles from the surrounding. Then, it was ground and sifted with an 80-mesh sieve. Resulting superabsorbent polymer powder was kept in desiccator for further use in moisture retention and soil amendment studies.

2.2.Measurement of Swelling Ratio
Dried hydrogel was used to determine the swelling ratio; an accurately weighed sample (0.1 g) was transferred to a tea bag with fine meshes (nylon screen) and then immersed entirely in distilled water (100 mL) at room temperature (20 °C±2). At regular time-intervals, the bag was taken out from the solution, the excess of water was wiped superficially with filter paper to remove surface-bound water and the bag was weighted. The same procedure was applied to measure the effect of temperature on the swelling equilibrium in the range of temperatures between 25-50 °C. The swelling ratio (Q) was obtained gravimetrically (Eq. 1): where W d and W s are the mass of the dry and swollen hydrogels, respectively.

2.3.Salt Solution Absorbency Measurements
The swelling properties of the used SAP at different ionic strength were also performed. Hydrogels were immersed in NaCl, Ca(NO 3 ) 2 and CaCl 2 solutions between 0 and 2.5 wt% concentrations to observe the swelling equilibrium at room temperature (20 °C±2). The determination of water absorbency of superabsorbent polymer in different saline solutions was similar to that in distilled water.

2.4.Effect of pH on Swelling Ratios of Used Hydrogels
The effect of pH on the equilibrium swelling ratio of the hydrogel was determined in buffer media of varying pH, ranging from 2 to 12. The measurement of water absorbency of superabsorbent in different pH solutions at room temperature (20 °C±2) was according to the earlier method described for swelling measurement in distilled water. Swelling capacity of the hydrogel at each pH was measured according to equation (1).

2.5.Characterization of the Superabsorbent Polymer
The FTIR spectra of the used SAP were performed on a Perkin-Elmer Spectrum Two Fourier-Transform Infrared Spectrophotometer under ambient conditions. Attenuated Total Reflection (ATR) mode was used. The spectral scanned range was 4000-500 cm -1 .

2.6.Water Retention Capacity in Soil with Used SAP
The intention of this test was to assess the effect of the hydrogel amendment on the moisture release curve of a sandy soil. The soil used in this study was taken from an agricultural area of the Ixtlahuaca region -EM, Mexico, characterized by intense vegetable production. The sample were air dried, sieved through a 2 mm sieve and oven dried at 70 °C for 24 h. Moisture release curves of soil (50 g) amended with different amounts of used SAP (0.5, 1 and 2 wt%) were evaluated. The soil without the amendment of the superabsorbent polymer was used as a control. Then, the mixtures were uniformly irrigated with 25 mL of distilled water and exposed at room temperature (20 °C±2). For each mixture, three replications were performed. These mixtures were weighed at certain intervals of time and the weight loss by water evaporation was registered. The water retention (WR) percentage of the soil with or without the hydrogel was calculated by the following formula (Eq. 2): where W o and W t are the initial mixture weight, and the mixture weight at a certain time, respectively.

2.7.Effects of Used Hydrogel on Germination Seeds
The purpose of this study was to explore the possible application of used SAP as water reservoir in agriculture, its direct effect on the cultivation of bean (Phaseolus vulgaris L.) and pumpkin (C. pepo) obtained from a commercial tree nursery in Ixtlahuaca -EM, Mexico, which were used in the essays applied in lab conditions (Hong and Lee, 2016). These species were chosen because they are grown in almost all Mexican territory, are found in a large range of ecological conditions and have economic importance, moreover they may be grown and consumed locally. These seedlings establish best in soils with adequate moisture. The used SAP was mixed at the rate of 0.5, 1 and 2% in dried soil. Then, 200 g of each of the sandy soil mixtures were filled in plastic pots (300 mL volume) with a filter paper placed at the bottom with a small hole. The sandy soil without the amendment of the hydrogel was used as a control. Four seeds each of bean and pumpkin were sown in triplicate pots for each of the soil and hydrogel combinations were used. The pots were then placed in plastic trays containing tap water at a depth of 6 cm and watered to ensure saturation. All the pots were placed in a dark room at the constant temperature (25 °C).
After two weeks, germinated seeds were counted and recorded. Emergence of shoot was taken as an indicator of seed germination. No water was applied except the initial saturation of the pots.
When there was evidence of wilting of seedlings appeared for the first time, the experiment was completed. The entire plant shoots and roots were then harvested and the shoot length was recorded, as well as fresh weight and dry weight. The germination percentage (GP) of the seeds was calculated as (Eq. 3):

3.1.Swelling Behavior of the Used SAP
Absorption capacity in grams of distilled water per gram of dry used superabsorbent polymer at different time intervals was measured. Initially, the rate of water uptake is very fast, and then it begins to level off. As observed in Figure 1, the swelling ability of the used hydrogel reaches its maximum value at 189 gg -1 in approximately 30 min. A time length of 30 minutes was thus considered as a reasonable and acceptable duration for complete hydrogel swelling, taking into account the possibilities of its applications in the agricultural field. According to the above results, may be attributed to the fact that superabsorbent polymer possess many hydrophilic groups, appropriate crosslinking degree and convenient three-dimensional network structure which generates osmotic pressure (Gao et al., 2013).

3.2.Effect of pH on Swelling Ratios of Hydrogels
The pH sensitivity of SAP polymer was studied by observing the equilibrium swelling (ultimate water absorption) at various pH values by using buffer solutions ( Figure 2). In acidic media, the most of carboxylate groups are protonated. This causes a decrease repulsion of anionic groups, which leads to a decrease in swelling ratio. In acidic condition, the carboxylic groups of the used SAP are protonated, causing a decrease in the repulsion of anionic groups and also a decrease in the swelling ratio. In neutral medium, the ionic strength is smaller than that in basic media, so provides higher swelling capacity (Gawande and Mungray, 2015). This is because the superabsorbent is anionic-type superabsorbent polymer, which contain a majority of hydrophilic groups, and which play an important role in swelling behavior and result in water absorbency changes through different interaction species in various pH solution. When the pH is 12, the increase in the ionic strength of the swelling medium causes the rapid decrease of the ionic osmotic pressure, also promoting the reduction in swelling ability in the equilibrium. It is important to indicate that the hydrogel can promote high swelling capacity at pH < 10, which is caused by the buffer action of -COOH and -COOgroups and this is a great advantage for the application of superabsorbent in agricultural fields. Similar conclusions could also be found in other works (Cheng et al., 2015).

3.3.Effect of Salt Solution on the Swelling Ratio of Used Hydrogel
It is more important to know the swelling capacity of a superabsorbent polymer when it contacts with various saline solutions considering its practical applications such as water release systems in agriculture. The equilibrium swelling of used SAP in different concentrations of NaCl, Ca(NO 3 ) 2 and CaCl 2 were investigated, as shown in Figure 3. When the SAP is placed in water, the maximum osmotic pressure is developed, and then the maximum swelling is achieved. The swelling ability of the superabsorbent in the three salts in aqueous medium was significantly decreased compared to the values measured in distilled water. This behavior, frequently observed in the swelling of all ionic hydrogels, can be attributed to a screening effect of the additional cations causing a non-efficient anion-anion electrostatic repulsion. This is also associated to a decreased osmotic pressure difference between the SAP network and the external solution resulting from the difference in the mobile ion concentration between the gel and aqueous phases Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [111] decrease and, consequently, the absorbency amounts are diminished. This finding is consistent with previous studies , the decrease in swelling is strongly dependent on the type and concentration of salt added to the swelling medium.

3.4.Effect of Temperature on Swelling Ratio of Used Hydrogel
The effect of environmental temperature on equilibrium swelling of SAP was determined in the range of temperatures between 25-50 °C. Hydrogel exhibits significant continuous changes in water content as a function of temperature (Zhang et al., 2015). It can be seen from Figure 4, the swelling ratio increase with temperature. The results given in Figure 4 can be explained by the fact that at 50 °C it is thermally more favorable for water molecules to form hydrogen bonds with polar groups in the SAP chains, allowing the hydrogel to exhibit maximum swellability. The results indicate that the swelling ratio increases with increasing temperature. In this connection, it must be pointed out that increasing temperature from 25 to 50 °C, the water absorption capacity increases significantly due to an increase in the segmental mobility of the hydrogel chains. At above 50 °C the swelling is drastically reduced. Therefore, the decrease in swelling capacity is induced by the breakdown Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [112] of the hydrogen bonds between the water molecules and the chains of the three-dimensional hydrogel network.

3.5.FTIR Analysis
The infrared spectroscopy was used to determine the type of bonds present in the used SAP as shown in Figure 5.

3.6.Water Retention Behavior in Soil-Superabsorbent Polymer
For agricultural use it is essential to evaluate the ability of the superabsorbent polymer to retain water in the soil for vegetation growth. Liquid water ensures the feeding of plants with nutritional elements, which increases the growth quality of plants. Figure 6 shows the water retention of soil mixed with SAP and soil without the SAP. The percentage of water retained by the soil, monitored for several days after an initial irrigation of a soil sample with different amounts of hidrogel was indeed significantly affected by the presence of the hydrogel, with higher water contents detected at selected time points, when increasing the hydrogel concentration. The rate of water loss increased with increased incubation time. The value of 100% corresponds to the initial weight of soil-hidrogel-water mixtures. It can be observed from Figure 6 that the amount of water in the control soil (without SAP) reaches zero after 12 days. After the addition of small hydrogel amounts (up to a maximum of 2%), the time length through which the soil remained humid almost triplicated, with respect to the soil without hydrogel. It is Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [113] clear that soil mixed with SAP can hold more water than soil without SAP. When soil is treated with SAP, its water retention properties are related to both porosity and the SAP's water absorbency. These results suggested that the adding of hydrogels into soil could obviously improve the water-retention capacity of soil and lessen the amount of water evaporation Liao et al., 2016). The trends of the curves suggest that the SAP improved the water retention properties of the treated soil during the early period. Similar effects of hydrogels on loamy soil water holding properties by Akhter et al. (2004), and in sandy soils by Dorraji et al. (2010) have been investigated.

3.7.Seed Germination and Seedling Growth
Seedling growth of both species, bean and pumpkin, was enhanced by the addition of used SAP in the sandy soil. Seed germination was considerably higher in 0.5-2.0% of SAP amended soils as compared to the control. As shown in Figure 7, the influence of used SAP on seed germination is evident; the controlled and sustained release of water allows plants to survive for several days without the need of further irrigation. As observed, the overall seed germination percentage of bean was slightly higher than pumpkin. The presence of SAP in the sandy soil drastically altered the height of the plants than of the not amended control with increments of 30, 127 and 399%, respectively, with 0.5, 1 and 2% of hydrogel in the case of bean (Phaseolus vulgaris L.), while for the pumpkin (C. pepo) the increases were 16, 59 and 178%, corresponding to 0.5, 1 and 2% of hydrogel in soil. The increase in soil moisture retention and improvement in seed germination and seedling growth as a result of superabsorbent hydrogel amendments has been reported previously (Orikiriza et al., 2013). The quantity of this increment depends on the quantity of hydrophilic polymer used (Dorraji et al., 2010). The proposed superabsorbent polymer improved retention of soil moisture and also the availability of water for the plants, decreasing the rate of moisture loss, so a delay of approximately 20-23 days at the wilting point was observed. This delay in the wilting point reduces the water requirement of plants. The germination energy of the seeds in soil with incorporation of hydrophilic polymer was obviously higher and denser than that of the seeds in soil without SAPs (Table 1 and Table 2). This is likely because the SAPs not only can absorb large amounts of water but also have good water retention capability, which supplies enough water to promote plant growth. The root and shoot biomass weights of both species was generally higher in SAP amended soil.   The results are means of three replicates; ±, standard deviation (SD), with P ≤ 0.05 were considered to be statistically significant Increase in plant growth can also be attributed to the increase of nutrient retention in the hydrogel -soil mixture, even though no fertilizer was applied to the plants in the present study. The positive effect of the hydrogel usage for the plant survival and growth of the plants has been reported in various studies (Orikiriza et al., 2013). Although further investigations should be performed to assess the hydrogel degradation chemistry and kinetics in the soil, the preliminary results of this lab study show promise for the possible use of recycled hydrogels as water reservoir in agriculture. With regard to the hydrogel degradation, analyses that are currently in progress seem to suggest that the hydrogel degradation occurs over a period of approximately 6 months (Demitri et al., 2013), without significantly altering the soil chemistry.

Conclusions
The superabsorbent polymer evaluated in this study showed the positive properties generally reported for others traditional hydrogels. The proposed SAP exhibited a high swelling ratio of 189 g g -1 of dry SAP. The results showed that, in salt solution, swelling ratio decreased with increasing ionic strength. The hydrogel exhibited a thermal and pH swelling behavior. Application of used superabsorbent polymer markedly improved retention capacity of soil for a longer time compared with control conditions. The use of the hydrogel from disposable diapers as water reservoir in the seed germination of bean and pumpkin in lab conditions was found to be advantageous and suitable for the sustained release of water to the soil and to the plant roots. These findings suggest that using SAP as soil conditioner will be useful for increased soil available water and consequently plant.