EVALUATING COMMON HEMATOLOGICAL AND BIOCHEMICAL BIOMARKERS AS PREDICTORS FOR ISCHEMIC HEART DISEASES AMONG ADULT SUDANESE PATIENTS

How to cite this article (APA): Babikir, A. O., Elmansour, O. K. A., and Altoum, H. A. M. (2022). Evaluating Common Hematological and Biochemical Biomarkers as Predictors for Ischemic Heart Diseases Among Adult Sudanese Patients. International Journal of Research GRANTHAALAYAH, 10(2), 76–84. doi: 10.29121/granthaalayah.v10.i2.2022.4491 76 EVALUATING COMMON HEMATOLOGICAL AND BIOCHEMICAL BIOMARKERS AS PREDICTORS FOR ISCHEMIC HEART DISEASES AMONG ADULT SUDANESE PATIENTS


INTRODUCTION
Ischemic heart diseases (IHD) are the number one cause of death, disability, and human suffering globally. It affects around 126 million individuals (1,655 per 100,000), which is approximately 1.72% of the world's population. Near nine million deaths are caused by IHD globally. With higher rates among men than women of a given age, CHD also carries a higher case-fatality rate among the male gender Khan et al. (2020). According to the latest WHO data published in 2018 IHD related deaths accounted for (20.07%) of total deaths with a death rate of 279.01 per 100.000 of the population Healthdata.org (2019). Numerous risk factors have been implicated in the disease process, morbidity and mortality, age, 77 gender, hypertension, diabetes, serum cholesterol levels, smoking, and excessive alcohol consumption NPS Medicine wise (2011), Norhammar et al. (2004), with a variable individual contribution of each risk factor between different communities or ethnic groups and remarkably strong consistency of the overall contribution of these risk factors to epidemiological studies. Bertazzo et al. (2013) A biomarker is tool used in predicting, diagnosing, and staging diseases, thus help effectively manage patients by the provision of prompt and accurate treatment and/or prognosis Biomarkers Definitions Working Group (2001), Yilmaz et al. (2010). A biomarker may be measured on a bio-sample (as a blood, urine, or tissue test), recorded (blood pressure, electrocardiogram, or Holter), or it may be an imaging test (echocardiogram). A vast array of biomarkers maybe added to conventional cardiovascular risk factors in predicting the risk of future CVD; however, the clinical value of some biomarkers is still questionable Threapleton et al. (2013). Currently, biomarkers that may reflect a higher risk of CVD include coronary artery calcification, carotid intima-media thickness, higher fibrinogen, and PAI-1 blood concentrations. elevated homocysteine, elevated blood levels of asymmetric dimethylarginine, Inflammation as measured by CRP, elevated LDL levels, and elevated blood levels of (BNP). Cihat et al. (2012)

MATERIALS AND METHODS
Study design: This is a descriptive cross-sectional case-control prospective analytical study to evaluate the haematological and biochemical predictors in ischemic heart disease patients at Almak Nimir University Hospital; a Shendi University-affiliated tertiary hospital in Sudan. Study population: A total of (100) samples were collected from the Study group of ischaemic heart disease patients and (100) samples were collected from healthy individuals as a control group. Inclusion criteria: Patients of both sexes with ischaemic heart disease (who take drugs or not take), irrespective of treatment patients with no other medical conditions were included in the study.
Exclusion criteria: Patients with other comorbid diseases such as renal failure, liver disease, haematological diseases and other medical conditions or receiving certain treatment that affect the results were excluded from the study. Data was collected using a self-administrated pre-coded questionnaire which was specifically designed to obtain information that helped in the study then analysed using SPSS version 11.5. (Mean, standard deviation, standard error mean, P. value by using independent T.test).
CBC was done by using Mindray Hematology Analyzer (Mindray bc-3000); every film was first inspected at low power (x10) before the general examination was undertaken with the x 40 lenses. The x100 oil immersion lens is generally reserved for examining. B. urea, S.creatinine and S. Ca2+ using automated chemistry analyzer, Mindray BS 120). Estimation of serum electrolyte using an ion-selective electrode. hs C.reactive protein and D.dimer were measured via sandwich immunodetection.

RESULTS
A total of (100) blood sample collected from ischaemic heart disease patients and (100) samples collected as control from healthy individuals include frequency of sex was 32 males (32%) and 68 females (68%), frequency of age groups 40-80 years 95(95%). Frequency of weight (50-100) kg (97%) in the study group.

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The average age of patients with ischaemic heart disease in the study was (61.44 ± 10.851), with a range of (40-80) years.
The majority of our patients (68) were of female gender (68%), and 32 (32%) were males. Regarding to weight, the average weight of patients with ischaemic heart disease in the study was (68.08 ± 11.912), with a range of (50-100 kg). Participation to risk factors to ischaemic heart disease reflected that; 64 (64%) were HTN patients, while 36 (36%) were not. On the other hand, 32 (32%) were DM patients, while the remaining 68 (68%) were not.

DISCUSSION
It is now well comprehended that a complex matrix of genetic, environmental, biological, and social factors contributes to the development and progression of IHD influencing the disease at all levels of encounter from risk stratification and development, to complication anticipation and outcome prognosis. Thus, it is vital to denote any variations in demographic characteristics and the relations of biomarkers to known risk factors. Batstone (1997), LaBaer (2005 While it's well acknowledged that among middle-aged people, coronary heart disease is (2) to (5) times more common in men than in women Bridget (2011). Our study results reveal the quite opposite, in which a total of 32 males (32%) and 68 females (68%) represented the study group. The world health organization estimated that (40%) of the variation in the sex ratios of coronary heart disease mortality was attributed to gender Jackson et al. (1999). One of the proposed explanations for the gender differences in CVD is hormonal difference. Studies have pointed out that Estrogen may have protective effects through glucose metabolism and the haemostatic system, and it may have a direct effect on improving endothelial cell function Linden et al. (1996). In terms of age distribution, (95%) of the study group aged (40-80 years). It is estimated that there is approximately a tripling of risk of IHD with each decade of life Sergio et al. (2013), and that (82 %) of people who die of CHD are (65yrs) and older Inaba et al. (2012).
Multiple hypotheses linked aging with ischemic heart diseases, for example the marked increase in serum total cholesterol levels with age; (45to 50 years) in males and (60 to 65 year) in females Jousilahti et al. (1999). Additionally, studies have described that the coronary vascular walls showed considerable mechanical and structural changes, causing progressively diminishing elasticity and compliance; as major contributors to IHD. Jousilahti et al. (1999), Toshio et al. (2001 The results of this study denoted that the hypertensive patients were at high risk to IHD and showed an increased prevalence as (64%) of the study group were found to be hypertensive, followed by diabetes as only (32%) were diabetic. Most of the patients were non-STEMI type, with predominantly inferior, anterior ECG changes.
Our study results demonstrated that there was a significant decrease in Hb, RBCs count, and PCV compared to control. (P-value >0.05). Results of this current study are different when compared to a study done by Toshio team in Japan which revealed that: high PCV and Hb were risk factors for IHD Puddu et al. (2002). Several factors related to RBCs are associated with IHD including Hb levels, PCV and ESR but there are not enough data to suggest a significant association between the RBCs count and cardiovascular disease. Patel et al. (2009) The study findings prevailed a decrease in the mean of RBCs indices (MCV, MCH & MCHC) and there was no association when compared to the control group (pvalue <0.05).
With the significant increase in the mean of RDW compared to the control group, there was a strong significant statistical value depicted among the study population; (P. value 0.000). These findings are in line with the study conducted by Patel VK et al, in which RDW was significantly associated with an increased risk of death secondary to CVD in cross-sectional studies of the population of the U. S Madjid and Fatemi (2013). In addition, the RDW is an independent predictor of death in patients who have had previous MI or stroke and in men referred for coronary angiography. Madjid and Fatemi (2013) The results of this study also confirmed an increase in the mean of WBCs & neutrophils and a decrease in lymphocytes, eosinophils & monocytes means with a significant statistical relationship found among the study population; (P. value 0.000). Results of the present study were in agreement with a previous study done by Mohammad Madjid, and Omid Fatemi suggesting that: leucocytosis can be considered as a marker of inflammatory changes in atherosclerotic lesions. Korean (2016) While our data implied an increase in the mean of platelet compared to the control group -with no significant statistical difference observed among the study population; (P = 0.070)-There was a considerable decrease in MPV mean compared to control, with strong significant statistical value demonstrated among study population; (P. value 0.000). It thus, appears that the role of platelets in the pathogenesis of IHD is due mainly to their functional properties and their interaction with plasma and tissue factors. This is coherent with the findings of various similar studies such as one conducted by Dong-Hyun Choi, Seong-Ho Kang and Heesang Song showing a significant correlation between MPV, the risk of ischemic stroke in AF patients, and poor clinical outcomes after PCI in patients with coronary artery disease. Volanakis (2001) 82 The results of the tests conducted showed an increase in the mean of hs-CRP compared to the control group. There was a strong significant statistical difference appeared among the study population; (P = 0.000). The recent study showed a strong association between hs-CRP and IHD. The results were in agreement with multiple other studies that presented an increase in CRP of IHD patients. One study denoted that: an increase in hs-CRP was associated with increased incidence of recurrent angina, coronary revascularization, and cardiovascular death. It has recently been suggested that hs-CRP is a marker of inflammation, along with serum cholesterol, which may be a critical component in the development and progression of atherosclerosis John et al. (2001). Our study also shows an increase in the mean of D.dimer compared to the control group with significant statistical differences estimated among the study population. This current study found that an increased D.dimer was associated with IHD. A pathophysiological explanation is that D.dimer is hypothesized to be involved in the so-called inflammation -coagulation -axis. The results of the present study are relevant to the previous study done by . Ostfeld et al. (2005) The increased mean of urea & creatinine was observed in this study to be higher than the control group. This study indicated an association between increased urea, creatinine, and IHD, in coherence with the result adopted by (Kirtane et al, 2005), suggesting that renal dysfunction has been associated with adverse cardiovascular outcomes Choudhury et al. (2011).
Finally, a significant reduction in this study in serum sodium (Na+), potassium (K+), and calcium (Ca2+) levels among the IHD patients were detected and compared to healthy individuals in the control group. This finding is in an accordance with the study carried out by Choudhury et al. (2011) which depicted an effect in (K+) level in IHD patients Yilmaz et al. (2010).

CONCLUSION
Our study concluded that Hb, PCV, red cells indices were lower in IHD patients, and that TWBCs, Neutrophil, platelet, plasma D. dimer and serum hsC-reactive protein were higher when compared to healthy individuals. IHD patients also had abnormally increased renal function tests (urea and creatinine) and a lower level of sodium (Na+), potassium (K+), and calcium (Ca2+) when compared to healthy individuals in the control group.