PHYSICO-CHEMICAL AND PHYTOCHEMICAL SCREENING OF n-HEXANE EXTRACT OF LEPTADENIA HASTATA LEAVES: A PROPOSED HERBAL REMEDY IN THE TREATMENT OF DIABETES MELLITUS
DOI:
https://doi.org/10.29121/granthaalayah.v7.i2.2019.992Keywords:
Leptadenia Hastata, Physico-Chemical, Phytochemical, Analysis, Elements, N-HexaneAbstract [English]
Background: Leptadenia hastata is a leafy, climbing plant of whose root, leaf and bark is widely consumed in Northern Nigeria. Decoctions made from the leaf is locally used in treatment of diabetes as well as for numerous trado-medicinal processes. This study investigates the physico-chemical as well as phytochemical compounds present in the n-hexane extract of Leptadenia hastata.
Method: Physical and chemical profile was determined using Smart Spectro test kits and a mass spectrometer to determine the presence of elements and physical properties.
Results: Phytochemical analysis revealed the presence of steroids, triterpenoids and cardiac glycosides. The elemental analysis revealed that Na, SO4 and PO4 had the highest levels in the extract. Pb, Hg and Cd were not detected which showed that the plant extract was not toxic. Other elements (NO4, Mn, Fe, K, Ni, Si, Cn, Zn, Ca, Mg, NH4, Cr and F) were present in varying concentrations which may contribute to its nutritive composition.
Conclusions: Various elements present in Leptadenia hastata may have either a direct or indirect role in the control and management of diabetes mellitus, since diabetes is associated with marked alterations in trace elements concentrations and may replace diminished element stores in the treatment of diabetes mellitus.
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References
Sigel, H. and Sigel,A., (Eds.) Metal ions in biological systems, Marcel Dekkar Inc. New York, Chap.3, pp.47-90 (1988).
Yamashita, C.I., Saiki M. and Sertié, J.A.A. Elemental analysis of leaves and extracts of Casearia medicinal plants by instrumental neutron activation analysis, Journal of Radioanalytical and Nuclear Chemistry, 2001, Vol. 270, No.1 181–186 DOI: https://doi.org/10.1007/s10967-006-0327-y
Miller, L.P. and Flemion, F., Phytochemistry, Inorganic elements and special group of elements, Vol. III, Litton educational publishing Inc., New York., pp. 1-40 (1973).
Sarma, B., and Goswami, B.C., Qualitative elemental analysis of some selected antidiabetic medicinal plants of Assam using X-ray Fluorescence (XRF) technique, Asian Journal of Plant Science and Research, 2016, 6(3):71-79
Siddiqui K., Bawazeer N., Joy S.S., Variation in macro and trace elements in progression of type 2 diabetes, Scientific World Journal, 2014 461591. DOI: https://doi.org/10.1155/2014/461591
Nourmohammadi I., Shalmani I.K., Shaabani M., Ladan G., Hossein N., Zinc, copper, chromium, manganese and magnesium levels in serum and hair of insulin-dependent diabetics, Archives of Iranian Medicine, 2000, 3(3), 88-100
Walter R.M. Jr, Uriu-Hare, J.Y., Olin, K.L, Oster, M.H., Anawalt, B.D., Critchfield, J.W., Keen, C.L., Diabetes Care, 1991,14,1050.
El-Yazigi A, Hannan N, Raines DA, Diabetes Res, 1991, 18, 129.
Bello, A., Aliero, A.A., Saidu Y. and Muhammad Phytochemical Screening, Polyphenolic Content and Alpha-Glucosidase Inhibitory Potential of Leptadenia hastata (Pers.) Decne. Nigeria. Bayero Journal of Pure and Applied Sciences. 2001, 19(2): 181-186.
Aliero, A. and Wara, S.H.. Validating the Medicinal Potential of Leptadenia hastata, African Journal of Pharmacy and Pharmacology.2009, 3(6):335-338.
Sanda, K.A., Sandabe, U.K., Auwal, M.S., Bulama, I., Bashir, T.M. Sanda, F. A., and Mairiga A.. Hypoglycemic and Antidiabetic Profile of the Aqueous Root Extracts of Leptadenia hastata in Albino Rats. Pakistan Journal of Biological Science. 2013, 16(4):190-4. DOI: https://doi.org/10.3923/pjbs.2013.190.194
Umaru, I.J., Ahmed, F.B., Umaru, H.A., and Umaru, K.I. Leptadenia hastata (Pers) Decne: Phytochemical, Pharmacological, Biotechnological, Botanical, Traditional Use and Agronomical Aspects, European Journal of Pharmaceutical and Medical Research 2018, 5(6):109-119.
Umaru, I.J., Badruddin, F.A. and Umaru, H.A. Phytochemical, antifungal and antibacterial potential of Leptadenia hastata stem-bark extract. MOJ Toxicol. 2018, 4(4):263‒268.
Azwanida N.N., A Review on the Extraction Methods Used in Medicinal Plant: Principles, Strengths and Limitations, Medicinal and Aromatic Plants, 2015, 4(196):1-6.
Williamson, E.M., Okpoko, D.T., Evans, F.J., (1996). Pharmacological Methods in Phytotherapy Research. John Wiley and Sons, Inc. Third Avenue, New York, USA. Pp. 155-167.
Sofowora A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum books Ltd. Ibadan, Nigeria. PP 191-289.
Mailafiya M.M. (2014) Phytochemical Studies and Effect of Methanol Leaf Extract of Leptadenia hastata (Pers.) Decne (Asclepiadaceae) on Acetic Acid Induced Writhes in Mice and Venom of Echis Ocellatus, being a Thesis Submitted to the School of Postgraduate Studies Ahmadu Bello University, Zaria.
Chukwudi E. I. and Yusha’u M., Phytochemical Screening and Brine Shrimp Lethality Assay of the Leaf Extracts of Cucurbita Maxima, Euphorbia Hirta, Leptadenia hastata and Mitracarpus Scaber, International Journal of Current Research in Life Sciences, 2016, 5(5):579-583.
Tan MJ and Ye Y, Anti-Diabetic Activities of Triterpenoids Isolated from Bitter Melon Associated with Activation of the AMPK Pathway, Science Direct, 2008, 15(3):263-273 DOI: https://doi.org/10.1016/j.chembiol.2008.01.013
Castellano, J.M., Guida, A., Delgado, T., Rada, M. and Cayeuela, J.A., Biochemical Basis of the Antibiotic Activity of Oleanolic Acid and Related Pentacyclic Triterpenes, Diabetes. 2013, 62(6):1791-1799.
Burdi, D.K., Qureshi, S. and Ghangaro, A.B.,. An Overview of Available Hypoglycemic Triterpenoids and Saponins to Cure Diabetes Mellitus, Advancements in Life Sciences 2014, 1(3):119-128.
Akinmoladun, A.C., Olaleye, M.T. and Farombi, E.O.,. Cardiotoxicity and Cardioprotective Effect of African Medicinal Plants, Toxicological Survey of African Medicinal Plants, 2014:395-421 DOI: https://doi.org/10.1016/B978-0-12-800018-2.00013-3
Wu Y. and Chen, S., Extraccted Triterpenes from Antrodia cinnamomea reduce the Inflammation to Promote Wound Healing via STZ Induced Hyperglycemia in Diabetic Mice Model, Frontiers in Pharmacology, 2016, 7:154 DOI: https://doi.org/10.3389/fphar.2016.00154
Putta, S., Yarla, N.S., Surekha C., Aliev, G., Divakara, M. B., Santosh, M.S., Ramu, R. Zameer, F., Chintala, R., Rao, P.V., Shiralgi, Y. and Dhananjaya, B.L., Therapeutic Potentials of Triterpenes in Diabetes and its Associated Complications, Current Topics in Medicinal Chemistry, 2016, 16(23): 2532-42.
Mukesh SS and Patil MB., Antidiabetic activity of Pongamia pinnata Leaf Extracts in Alloxan-Induced Diabetic Rats. International Journal of Ayurveda Resources, 2010 1(4):199–204. DOI: https://doi.org/10.4103/0974-7788.76780
Sani, U.M.,, Phytochemical Screening and Antidiabetic Effect of Extracts of the Seeds of Citrullus lanatus in Alloxan-induced Diabetic Albino Mice Journal of Applied Pharmaceutical Science, 2015, 5 (3):051-054 DOI: https://doi.org/10.7324/JAPS.2015.50309
Khan, A. R. and Awan, F. R., Metals in the pathogenesis of type 2 diabetes, Journal of Diabetes & Metabolic Disorders, 2014, 13:16, 1-6. DOI: https://doi.org/10.1186/2251-6581-13-16
Tapiero H., Tew K.D., Trace elements in human physiology and pathology: zinc and metallothioneins, Biomed Pharmacother, 2003, 57, 399-411. DOI: https://doi.org/10.1016/S0753-3322(03)00081-7
Khan, K. Y., Khan, M. A., Niamat, R., Shah, G. M., Fazal, H., Seema, N., Hussain, I., Ahmad, I., Inayat, H., Jan, G. and Kanwal, F. ) Elemental Content of Some Anti-Diabetic Ethnomedicinal Species of Genus Ficus Linn. Using Atomic Absorption Spectrophotometry Technique, Journal of Medicinal Plants Research. 2012, 6(11):2136-2140 DOI: https://doi.org/10.5897/JMPR11.1276
Schlienger, J. L., Grunenberger, F. and Maier, E.A.. Disorders of Plasma Trace Elements in Blood Glucose Equilibrium. Press eMedicine 1998, 17:1076-1079.
Swimnathan, S., Fonseca, V.A., Alam, M.G. and Shah, S.V.. The role of Iron in the Diabetes and Its Complications. 2007, Diabetes Care 30(12):1923-33. DOI: https://doi.org/10.2337/dc06-2625
Djama, A.A.D. Djama, Kouassi-Goffri, M.C., Ofosu, F.G. and Aboh, J.K., Heavy Metal Analysis of Some Anti-Diabetic Medicinal Plants in Côte d'Ivoire, Current Research Journal of Biological Sciences. 2012, 4(5): 633-637.
Rajendra A., Narayan V., Granavel I., Study on the analysis of Trace elements in Aloe Vera and its biological importance, Journal of applied sciences Research, 2007, 3(11), 1476-1478.
Oyedeji, S. O., Adesina, A.A., Oke, T.O. and Tijani, Y.O., Evaluation of Essential Trace Metals in Female Diabetes Mellitus Patients in Nigerian Population, African Journal of Biotechnology, 2014, 13(18):1910-1914.
Mooradian A.D., Morley J.E, Micronutrient status in diabetes mellitus, American Journal of Clinical Nutrition, 1987, 45(5), 877-895. DOI: https://doi.org/10.1093/ajcn/45.5.877
Umaru, I.J., Badruddin, F.A. and Umaru, H.A., Phytochemical, antifungal and antibacterial potential of Leptadenia hastata stem-bark extract. MOJ Toxicol. 2018, 4(4):263‒268.
Missaoui, S., Rhouma, K.B., Yacoubi, M.T., Sakly, M. and Terourbi, O., Vanadyl Sulfate Treatment Stimulates Proliferation and Regeneration of Beta Cells in Pancreatic Islets, Journal of Diabetes Research 2014:1-7. DOI: https://doi.org/10.1155/2014/540242
Piero M.N., Njagi J.M., Kibiti C.M., Ngeranwa J.J.N., Njagi E.N.M. and Miriti P.M., The role of vitamins and mineral elements in management of type 2 diabetes mellitus: A review, South Asian Journal of Biology Sciences, 2012, 2, 107- 115.
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