INTRODUCING METHODOLOGY TO DETECT DEAD TISSUE STORED ENERGY

 

Abraham A. Embi ¹

 

¹ BS MBA,13442 SW 102 Lane Miami, 33186, Florida, United States

 

 

 

 

1. INTRODUCTION

The main purpose of this manuscript is to introduce the presence of energy emitted by dead tissue harvested from three small reptile’s tails (lizards). A glass slide optical microscopy method is used to examine dead and living lizard tails tissue. The first tail found attached to a skeleton that was adhered for approximately four weeks to an outer cement wall by a rear limb (no exposure to soil); the second and third obtained after forced detachment during manual trapping and then released, used as controls (Exhibit 1). All samples were tested for the presence of the ubiquitous enzyme catalase present in living tissue; all testing positive for catalase (including the dead) by adding 3% hydrogen peroxide (H2O2) drops, oxygen bubbles were detected as shown Figure 1, Figure 2.

Exhibit 1

                                                                     

Exhibit 1 Showing A, B: Dead Lizard Leg Limb Adhered to Outer Wall for Approximately One Month. Specimen Was Detached and Tested for The Presence of Catalase. C: Second Fresh Lizard Tail D: Third Fresh Lizard Tail

 

 

 

2. VIDEO-FRAME SHOWING VIGOROUS O2 BUBBLES FORMING POST ADDITION OF 3% H2O2 TO FRESHLY OBTAINED TAIL FRAGMENT

Figure 1

                                                                       

Figure 1 H2O2 Drop on Fresh Living Lizard Tissue Tail- Vigorous Oxygen Bubbles Supporting Presence Catalase For details link to:  https://youtu.be/2t0QBs---JI    Or Scan QR Code in Left Lower Corner

 

3. MONTH OLD LIZARD SKELETON TISSUE FRAGMENT IN LIQUID SHOWING REMMANT OF MAGNETIC ATTACTION

Figure 2

                                                                       

Figure 2 Month-Old Lizard Skeleton Tissue Fragment Showing Remmants of Magnetic Attraction. Please Scan QR Code for Details

 

4. MONTH OLD LIZARD SKELETON TISSUE TESTING POSITIVE FOR CATALASE

Figure 3

                                                                     

Figure 3 Showing Less Vigorous Oxygen Bubbles Post Drops Of 3% H2O2 On Dead Dehydrated Lizard Tissue (Shown in Figure 3) Exposed to Weather/Sun Exposure For 4 Weeks. Bubbles Supporting the Presence Catalase

 

5. MATERIALS AND METHODS

5.1. Materials

1)     Glass slides 25x75x1 mm

2)     Video microscope

3)     Catalase powder

4)     Wooden toothpicks

5)     Small transfer pipette

6)     Demineralized water

 

5.2. Methods

Tissue sample fragments from one long-term dead lizard tail analysed. Two additional freshly (approximately 4 hours) detached lizard’s tails were secured and studied.

Using a wooden toothpick on a clean glass slide approximately a pinch (50 small crystals of K3Fe) was placed. Two drops of demineralized bottled water placed on crystals; then gently mixed with same toothpick until all crystals have dissolved. The solution is then evenly spread on the slide, and a small fragment of tissue placed in the center. As the fluid evaporates crystallization ensues, video and still images recorded for further analysis.

 

 

 

6. PLUCKED HAIR FOLLICLE .EXAMPLE OF K3Fe TOTALLY ABSORBING INCOMING EMFs. NOTICE COHESIVE CRYSTALS

Figure 4

                                                                     

Figure 4 Black Arrow: Precipitated Crystals of Potassium Ferricyanide by Incoming Electromagnetic Radiation from A Human Hair Follicle, (Image from Hair Follicles Files)

 

7. RESULTS

 Both dead and alive lizards tissue tails when tested by TTM and shown to exhibit emission of electromagnetic energy. Two fresh tail living samples used as control. The one-month dead lizard skeleton tail displaying what appears to be less dense crystals Figure 5 theorized as result of a weaker EMFs signals. The more recent detached tissue energy sensed by the K3Fe showing a more defined and dense semi-circular crystallization images Figure 6.

 

NOTE: Supporting additional testing shown demonstrating weaker EMFs triggering weaker Potassium Ferricyanide crystals depositions.

 

Figure 5

                                                                     

Figure 5 Amplified K3Fe Crystals Precipitated from Dead Tissue Emfs. Black Arrow: Notice Space (Less Cohesive) Between Crystals Formation For Additional Details, Please Link To: https://youtu.be/0EXN0iBs-18       Or Scan QR Code in Left Upper Corner of Image Recommended to Fast Forward Video to Frame 03’ 55” To Fully Appreciate Effect of Weaker Emfs on Precipitating K3Fe Crystals

 

8. SELECTED IMAGES SHOWING MARKED DIFFERENCE BETWEEN CRYSTALIZATION PATTERNS BETWEEN:            A: WEAK EMFs and B: STRONGER EMFs

Figure 6

                                                                      

Figure 6 A: Black Arrow. One month old dead lizard tail triggering less dense crystals. B: Black Arrow: Tail fragment from four hours post detachment showing a more cohesive-compact crystals formation.

 

9. ADDITIONAL TEST DONE ON A CUT FRESH DISTAL TAIL FRAGMENT SHOWING STRONGER EMFs CLOSER TO SOURCE TRIGGERING COHESIVE CRYSTALS.

Figure 8

Figure 7 Sample 3 in Exhibit 1. Small Lizard Distal Tail Tissue in SSP K3Fe Showing Potassium Ferricyanide Crystals Totally Absorbing Emfs. Notice The Further Away from The Emfs Source (Tail Fragment) The Less Cohesiveness or Compactness of The Crystals. Black Arrow: Pointing at K3Fe Crystals. For Details Link To https://youtu.be/Q-yNH7sc3m8     Or Scan QR Code in top left of image

 

10. CUT-PASTE VIDEO FRAMES EXPANDED IMAGE OF FIGURE 5

Figure 8

                                                                     

Figure 8 Small freshly cut lizard distal tail tip in SSP K3Fe showing increasing crystals adhesion when nearing the EMFs source. A, B, C: Further away from tip- Notice greater dispersion of crystals distribution.  D, E: Near the tail tip (EMF source). Notice crystals compactness. For additional details, please Scan QR Code in top left corner of figure

 

11. DISCUSSION

Prior research by this author showed catalase proper to be an emitter of electromagnetic radiation demonstrated Embi (2018) by using a novel tabletop optical microscopy methodology (TTM) able to detect incoming electromagnetic radiation Scherlag et al. (2016). The main component of the TTM is diluted Potassium Ferricyanide (K3Fe) crystals in drops of water. K3Fe exhibits “total absorption of incoming electromagnetic radiation” (EMFs) Baranov et al. (2015), Figgis et al. (1969), therefore, a fit to detect tissue energy in dead or alive biological samples via In Vitro experiments previously described in a paper published in 2016. In other words, when drops of K3Fe in solution detect incoming radiation, the steady progress of K3Fe crystallization and crystals densities is delayed and their compactness have been observed to be directly proportional to a shorter distance from the incoming electromagnetic radiation strength Embi (2020). This newly introduced observation is easily seen in Figure 6 above. The farther from the EMF source the weaker the signal, and crystals compactness. The repetition of organized K3Fe semi-circular crystals is seen as periodic emissions of EMFs by the tail tip. The same phenomenon (periodic emissions) was previously found emitted by freshly plucked hair follicles Figure 4.

 

12. SUMMARY AND CONCLUSIONS

Catalase has been identified as essential in tissue metabolism; its role in breaking down toxic chemicals during cell respiration is of paramount importance in cell survival. In this manuscript the presence of catalase is also demonstrated in dead tissue as evidence is presented on ≈ one-month dead lizard tissue fragment. Energy emitted by the dead tissue is displayed as precipitating crystals formation in liquid K3Fe on a glass slide. Images are presented documenting a direct relationship between EMFs intensity and K3Fe crystals distribution, in other words the weaker the EMFs the less dense precipitation of K3Fe crystals Figure 4, Figure 5, Figure 6, Figure 7, Figure 8 + videos). Biological tissue energy is shown to be present in dead and living similar tissue samples. Further research is advised.

 

13. Additional Commentary

As often happens in research unexpected additional information is documented; in this manuscript the distal tip of a reptile tail (lizard) is shown as a point of energy discharge of EMFs. This is appreciated when running the complete video recording in Figure 8. The distal end showing organized semi-circular concentric EMFs emissions, whereas the cut end lacks that characteristic. It could be stated “In reptiles, the tail tip is identified as a point where EMFs are emitted. Is the tail also a receiving point detecting external energy sources?

 

 

 

 

 

 

 

 

 

14.  Questions Arise

First question:

What is the role of catalase in dead tissue?

In this manuscript a new finding is presented, the detection of energy stored in dead biological tissue attributed to catalase. Role unknown only hypothesized (see reply to question 2 below).

 Second question:

If catalase has been identified as an essential in the living by neutralizing toxic reactive oxygen species; then what if any is its role in the dead?

In this manuscript, the presence of energy in dead tissue could be hypothesized to be a signalling mechanism attracting scavengers.

 

15. SUPPLEMENTARY BONUS INTERESTING CURIOUS FINDING SHOWING DISLODGED FAT CELL FROM PROXIMAL LIZARD TAIL EMITTING ENERGY DELAYING ADVANCE OF K3FE CRYSTALIZATION. ALSO, CRYSTALS PIERCING CELL OUTER MEMBRANE

  Figure 9

                                                                     

Figure 9 Lizard tail shown in Exhibit I (Sample 3) immersed in drops of K3Fe. Round structure (Possible Fat Cell) migrating towards ADVANCING forming crystals. Crystals seen piercing outer membrane of structure- Also noticed is delay in crystallization advance, possibly indicating electromagnetic energy emitted by fat cell. For details link to: https://youtu.be/zoPhBH_-fHc     Or Scan QR Code in left upper corner of image.

 

CONFLICT OF INTERESTS

None. 

 

 

ACKNOWLEDGMENTS

None.

 

REFERENCES

Baranov, D. G., Edgar, J. H. , Hoffman, T., Bassim, N., and Caldwell, J. D. (2015). Perfect Interferenceless Absorption at Infrared Frequencies by a Van Der Waals Crystal. Physical Review B, 92 (20). https://doi.org/10.1103/PhysRevB.92.201405

Embi, A. A. (2018). “Catalase Intrinsic Emissions Of Electromagnetic Fields As Probable Cause in Cancerogenesis from Consumption of Red and Processed Meat.” International Journal Of Research - Granthaalayah, 6(8), 33-40. https://doi.org/10.29121/granthaalayah.v6.i8.2018.1259

Embi, A. A.  (2020). The Human Hair Follicle Pulsating Biomagnetic Field Reach as Possible Additional Factor in Migraine Headaches a Biophysics Based Hypothesis. International Journal of Research -Granthaalayah, 8(5), 221-229. https://doi.org/10.29121/granthaalayah.v8.i5.2020.179

Figgis, B. N., Gerloch, M., Mason, R., and Nyholm, R. S.(1969). The Crystallography And Paramagnetic Anisotropy of Potassium Ferricyanide.  https://doi.org/10.1098/rspa.1969.0031

Scherlag, B. J., Sahoo, K., and Embi A. A. (2016). A Novel and Simplified Method for Imaging The Electromagnetic Energy in Plant and Animal Tissue. Journal of Nanoscience and Nanoengineering 2016, 2 (1), 6-9.