2.    MATERIALS AND METHODS

2.1. Materials

Potassium Ferricyanide K₃[Fe(CN)₆].

25x75x1 mm glass slides

Fresh human blood smear

Freshly in toto plucked scalp human hair

Demineralized bottle water

Celestron Video Microscope Model # 44348

MacBook Pro Apple computer with Photo Application software.

 

2.2. Methods

1)     A fresh human blood smear obtained. Solution prepared by diluting approximately 2 milligrams of (K₃Fe) diluted in one drop of demineralized water was added to the center of a wet smear*.

Note: [1] * A finger stick allowed for the milking of two drops of blood, then placed on a clean 25x75x1 mm glass slide. The mechanical smear was done as per published instructions from the USA center for disease control. There is a time window of approximately 60±20 seconds for a complete preservation of in vivo properties of the blood tissue. For details link to: (https://doi.org/10.5281/zenodo.3472760)

2)     One freshly tweezers plucked scalp hair was carefully placed in approximately the area where the of the liquid K₃Fe had displaced the blood tissue.

3)     The preparation allowed evaporating, Images recorded and stored for analysis.

 

3.    RESULTS AND DISCUSSIONS

Prior research by this author showed the follicle and shaft magnetic fields properties on a glass slide (in the absence of blood) with the addition of only liquid K₃Fe, as shown in images showing the hair shaft unilateral presence of electromagnetic radiation (EMR) (Embi (2018)) (Figure 2). In this manuscript, qualitative images are presented where not only the bipolar electrical property of the shaft is documented; but also showing EMR originating from the hair follicle routed only to one side of the shaft, a gap in the hair follicle’s EMR continuity accounting for the shaft’s bipolarity (+ −) (Figure 2, Figure 3).

 

Potassium as Immunosuppressing Agent

The rejection of several exogenous materials, such as a hair follicle, keratin flakes and small iron filings amongst others by a fresh human blood smear had been described, Figure 7  ( Embi (2018)).

In this manuscript, the addition of liquid K₃Fe to a fresh human blood smear inhibited rejection of an in toto hair (follicle and shaft). This inhibition allowed for images such as in (Figure 2, Figure 3, Figure 4, Figure 5). The question arises: What is the mechanism whereby addition of liquid K₃Fe to a fresh blood smear inhibits fresh blood from rejecting a hair follicle?

 

Perhaps some elucidation could be explained by findings where a link between tumor-induced immune suppression by the Potassium ion (K⁺) exists (Vodnala et al. (2019)).

 

 K₃Fe Total Absorption of Incoming EMRs

Hair Shaft Polarity and Blood Coagulation

Liquid K₃Fe allowed for the display of hair follicle and shaft EMR as shown (Figure 2, Figure 4). A second important observation is the  findings or previous papers correlating a positive charge with blood coagulation. Early in 2018, an image was recorded showing what appeared to be a one-sided electrical discharge in a human hair shaft. (Figure 1). In 2018 there was not enough published experimental data found by this author to support a hair shaft bipolarity finding.

The question arose: How could a keratin surrounded filamentous structure express opposite sides charges?

 

Prior Publication Hinting at Bipolarity

Potassium Ferrocyanide Spatially Detachment of Shaft Exo-Cuticles

A paper published in 2016, showed that when a human hair shaft was sandwiched between two glass slides and covered by Potassium Ferrocyanide (Embi (2016)), post evaporation, microscopy images showed an unexplained phenomenon, being the spatially separated images of hair exocuticles. In other words, there was a need of a microscope depth of field adjustment to bring the cuticles layers in focus as shown in Figure 6 below.

Evidence shows that the addition of one drop of liquid K₃Fe onto a fresh blood smear inhibited rejection of a foreing material, namely a plucked human hair. This inhibition, allowed for the identification of the hair EMRs. This is supported by a property of K₃Fe being the “Full absorption od incoming EMRs” ( Figgis et al. (1969),  Baranov et al. (2015))

The hair unique electromagnetic radiation pattern is shown partially engulfing the follicle. There is a “gap” shown in the images that does not allow the magnetic signal from traveling to one side of the hair shaft. The Hair Shaft Inherent Unilateral Divergent Charges (+ −) are displayed.

 

The Dual Consequences of Follicle’s EMR Gap

First) The abrupt interruption of EMRs in one side of the hair follicle, accounts for the hair shaft’s one-sided EMR activity triggering positive charges.

Second) The genesis of a visual display resembling a “Shepherd Hook” pattern of the human hair EMR emissions.

 

 

 

 

 

Figure 1 Hair shaft sanwiched between glass slides and covered by drops potassium Ferrocyanide in solution. Showing shaft unilateral discharge of elecromagnetic energy. Black Arrow: Direction of forces- X: Shaft side void of energy.

 

Image reproduced from: Cite This Article: Abraham A. Embi Bs. (2018). “THE SHEPHERDS HOOK PHENOMENON PATTERN OF HAIR ROOTS A DEMONSTRATION OF COMPARATIVE BIOLECTROMAGNETISM BETWEEN HUMAN HAIRS AND MOUSE WHISKERS BY MEANS OF THE PHOTOELECTRIC EFFECT.” International Journal of Research - Granthaalayah, 6(7), 317-326. https://doi.org/10.29121/granthaalayah.v6.i7.2018.1312.  

Figure 2 Image of hair imprint surrounded by human blood tissue mixed with potassium Ferricyanide crystals. Notice the EMR unable to fully reach one side of the hair shaft (orange line).

 

Image reproduced from:  Embi, AA (2021). Some curious findings hair follicles bioelectromagnetic radiation expressed as light displacing matter in its path and the contralateral emission of magnetic fields found in the hair shaft. International Journal of Research - GRANTHAALAYAH, 9(7), 334. doi: 10.29121/granthaalayah.v9.i7.2021.4114

 

Another Hair Placed on Human Blood Smear Plus Liquid Potassium Ferricyanide Drops

Figure 3 N=2 Hair on fresh human blood smear, post addition of K3Fe allowing for this demonstration. Black Arrow: Pointing at mix of  K3Fe crystals and coagulated blood surrounding follicle prior removal via tweezers.

 

For further details link to: https://youtu.be/LLz43yAbpg0….or Scan QR Code in left upper corner of image

Same Hair as in Figure 3 Above. Hair Removed via Tweezers Showing Follicle’s Imprint Delineating EMRs Abruptly Ending Prior to Surrounding the Follicle. Red line indicating, “gap” void of EMRs.

Figure 4 image introduced in this manuscript showing Hair EMR outline after hair removed. Black Arrows: Pointing at mix of crystals and coagulated blood plus edge of blood smear. The abrupt end of EMRs circumventing the follicle is shown.

 

For details link to: https://youtu.be/LLz43yAbpg0….or Scan QR Code in left upper corner of image.

 

Amplified Image of Figure 4 above

Figure 5 Amplified image of Fig 4, showing X= Some blood tissue trapped under hair follicle. Brackets= Showing mix of blood tissue (RBCs) and fibrin.

 

Image published in 2016 Hinting at Bipolarity of Hair Shaft Exocuticles

 

Figure 6 Microphotograph of hair in contact with Prussian Blue (PBS) between two glass slides (SDW) after evaporation, showing: S= Shifting shaft showing damage caused by tweezers, C1 = In focus damaged anterior level cuticles, C2 = Out of focus damaged posterior level cuticles (out of focus). X4 Magnification.

 

Image reproduced from:

Embi AA. Adhesion Failure of External Hair Cuticles Caused by Prussian Blue: Possible Electrochemical Roles of Sulfur and Cystine. J Nat Sci, 2(6):e194, 2016.

 

 

 

 

 

Image of Fresh Human Blood Smear Repulsing Hair Follicle

Figure 7 Image depicting fresh human blood smear rejecting  hair follicle. F= Follicle. X= Empty space due to repulsive phenomenon.

 

Image reproduced from: Cite This Article: Abraham A. Embi Bs. (2018). “BIOMAGNETISM AS FACTOR IN RED BLOOD CELLS DEFORMATION.” International Journal of Research - Granthaalayah, 6(12), 46-57. https://doi.org/10.29121/granthaalayah.v6.i12.2018.1245.

 

Image published in 2018 of Hair Shaft on Fresh Blood Smear

Showing Effect of Positive Pole on Coagulation

Figure 8 Microphotopraph depicts detached hair shaft outline.

 

A= Non-coagulated blood  S= Hair shaft    C= Coagulated blood.

(-)= Negative pole RBC repulsion. (+)= Positive pole attracting RBCs (coagulation). Reproduced from:

 Abraham A. Embi Bs. (2018). “HAIR AND BLOOD ENDOGENOUS LOW LEVEL BIOMAGNETIC FIELDS CROSS-TALK EFFECTS ON FIBRIN INHIBITION AND ROULEAU FORMATION.” International Journal of Research - Granthaalayah, 6(11), 200-208. https://doi.org/10.29121/granthaalayah.v6.i11.2018.1118.

 

4.    SUPPLEMENTAL INFORMATION

Upon re-visiting my files, found some video-recordings where the hair was physically removed from the glass smear- Since the hair had been covered by Potassium Ferricyanide (K₃Fe) in solution; and K₃Fe has the property of full absorption of incoming electromagnetic radiation (EMR), the hair outer layers EMR are shown as K₃Fe crystals. These images are introduced for the first time in this manuscript- Notice the consistent narrowing between the distal follicle and the bulb-

 

Figure 9 Showing sequential images from video-recordings outlining the human hair external  electromagnetic radiation. Black Arrows: Notice the narrowing shown between the distal follicle and the bulb also showing a gap in energy continuity. This gap is theorized to induce the bipolar nature of the shaft (+-). TIBS= Temporary In Vivo Blood Smear. Orange Arrows: Pointing at area where shaft exits towards skin.

 

Figure 10 Cut and Paste frames to illustrate the human scalp hair magnetic imprint post hair removal from slide. Scalp hair in fresh blood smear mixed with liquid Potassium Ferricyanide. After drying, outer layers of hair are delineated showing hair anatomical areas. Human hair magnetic Imprint on glass slide. Drawing on right side of figure reproduced as described in the copyright fair use doctrine.

 

Credit of drawing  given to: Pisal Rishikaysh et al. DOI: doi:10.3390/ijms15011647

For video detais link to:: https://youtu.be/LLz43yAbpg0  Or scan QR Code in left side of Figure 4.

 

REFERENCES

Abraham A. Embi Bs. (2018). “BIOMAGNETISM AS FACTOR IN RED BLOOD CELLS DEFORMATION.” International Journal of Research - Granthaalayah, 6(12), 46-57. Retrieved from https://doi.org/10.29121/granthaalayah.v6.i12.2018.1245.

Abraham A. Embi Bs. (2018). “THE HUMAN HAIR FOLLICLE PULSATING BIOMAGNETIC FIELD REACH AS MEASURED BY CRYSTALS ACCRETION.” International Journal of Research - Granthaalayah, 6(7), 290-299. Retrieved from https://doi.org/10.5281/zenodo.1341349.     

Abraham A. Embi Bs. (2018). “THE SHEPHERDS HOOK PHENOMENON PATTERN OF HAIR ROOTS A DEMONSTRATION OF COMPARATIVE BIOLECTROMAGNETISM BETWEEN HUMAN HAIRS AND MOUSE WHISKERS BY MEANS OF THE PHOTOELECTRIC EFFECT.” International Journal of Research - Granthaalayah, 6(7), 317-326. Retrieved from https://doi.org/10.29121/granthaalayah.v6.i7.2018.1312.  

B. N. Figgis, Malcolm Gerloch, Ronald Mason, and Ronald Sydney (1969) Nyholm the crystallography and paramagnetic anisotropy of potassium ferricyanide. https://doi.org/10.1098/rspa.1969.0031 Retrieved from DOI: https://doi.org/10.1098/rspa.1969.0031  

Cohen, D., Palti, Y., Bn, C. & Sj, S. (1980). Magnetic Fields Produced By Steady Currents In The Body. Proc. Natl. Acad. Sci 77(3), 1447–1451. Retrieved from https://doi.org/10.1073/pnas.77.3.1447

Embi AA (2016). Adhesion Failure of External Hair Cuticles Caused by Prussian Blue: Possible Electrochemical Roles of Sulfur and Cystine. J Nat Sci, 2(6):e194.

D. G. Baranov, J. H. Edgar, Tim Hoffman, Nabil Bassim, Joshua D. Caldwell (2015). Perfect interferenceless absorption at infrared frequencies by a van der Waals crystal. DOI: 10.1103/PhysRevB.92.201405 Retrieved from DOI: https://doi.org/10.1103/PhysRevB.92.201405  

Scherlag, B.J., Sahoo, K., Embi, A.A (2016). A Novel and Simplified Method for Imaging the Electromagnetic Energy in Plant and Animal Tissue. Journal of Nanoscience and Nanoengineering 2(1): 6-9.

Schneider MR, Schmidt-Ullrich R, Paus R (2009). The hair follicle as a dynamic miniorgan. Curr Biol. Feb 10;19(3): R132-42. Retrieved from doi: 10.1016/j.cub.2008.12.005. Retrieved from    https://doi.org/10.1016/j.cub.2008.12.005

Vodnala S.K., Eil R., Kishton R.J., Sukumar M., Yamamoto T.N., Ha N.H., Lee P.H., Shin M., Patel S.J., Yu Z., Palmer D.C., Kruhlak M.J., Liu X., Locasale J.W., Huang J., Roychoudhuri R., Finkel T., Klebanoff C.A., Restifo N.P (2019). T cell stemness and dysfunction in tumors are triggered by a common mechanism.Science. Mar 29. Retrieved from https://doi.org/10.1126/science.aau0135