SCREENING OF BACTERIOCIN PRODUCING BACILLUS PUMILUS PB1 ISOLATED FROM THE GUT OF TIGER SHRIMP, PENAEUS MONODON
DOI:
https://doi.org/10.29121/shodhkosh.v5.i5.2024.4644Keywords:
Probiotics, Bacteria, Shrimp, Gut, Bacteriocin, AntagonisticAbstract [English]
In the present study, probiotic bacterial isolates were isolated from the gut of Penaeus monodon. The isolated probiotics showed activity against the marine bacterial pathogens. The gut bacterial isolates were further screened for acid production test and only nine bacterial isolates were produced secondary metabolites. Based on morphological, biochemical and physiological factors the selected bacterium was identified as Bacillus pumilus PB1. The secretion of the metabolite was found only at the stationary period of the bacterial growth. It was found that the metabolite was detected at the incubation period of 2 to 3 days and metabolite production started to deplete after 5th day. Hence, optimum fermentation period for secondary metabolite production was 72 h of incubation at room temperature. Of the pH level tested, production of bioactive metabolites and biomass were maxium at pH 7.0. From the experiment it was very clear that the selected bacterial isolate showed maximum growth at 30 °C. mong the carbon sources tested, glucose favoured as good carbon sources for its growth and so preferred as good carbon source for bioactive metabolite production. Among the tested nitrogen sources, glycine enhanced the growth of bacteria and metabolite production. Among the ionic sources, supplemented Ca2+, Mg2+ and Mn2+ supported the growth of the bacterium.
References
Rengpipat, S., Phianphak, W., Piyatiratitivorakul, S., & Menasveta, P. (1998). Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture, 167(3-4), 301-313.
Rengpipat, S., Phianphak, W., Piyatiratitivorakul, S., & Menasveta, P. (1998). Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture, 167(3-4), 301-313. DOI: https://doi.org/10.1016/S0044-8486(98)00305-6
Boonthai, T., Vuthiphandchai, V., & Nimrat, S. (2011). Probiotic bacteria effects on growth and bacterial composition of black tiger shrimp (Penaeus monodon). Aquaculture Nutrition, 17(6), 634-644. DOI: https://doi.org/10.1111/j.1365-2095.2011.00865.x
Rengpipat, S., Rukpratanporn, S., Piyatiratitivorakul, S., & Menasaveta, P. (2000). Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture, 191(4), 271-288. DOI: https://doi.org/10.1016/S0044-8486(00)00440-3
De, D., Ananda Raja, R., Ghoshal, T. K., Mukherjee, S., & Vijayan, K. K. (2018). Evaluation of growth, feed utilization efficiency and immune parameters in tiger shrimp (Penaeus monodon) fed diets supplemented with or diet fermented with gut bacterium Bacillus sp. DDKRC1. isolated from gut of Asian seabass (Lates calcarifer). Aquaculture research, 49(6), 2147-2155. DOI: https://doi.org/10.1111/are.13669
Mongkol, P., Bunphimpapha, P., Rungrassamee, W., Arayamethakorn, S., Klinbunga, S., Menasveta, P., & Chaiyapechara, S. (2018). Bacterial community composition and distribution in different segments of the gastrointestinal tract of wild‐caught adult Penaeus monodon. Aquaculture Research, 49(1), 378-392.
Mongkol, P., Bunphimpapha, P., Rungrassamee, W., Arayamethakorn, S., Klinbunga, S., Menasveta, P., & Chaiyapechara, S. (2018). Bacterial community composition and distribution in different segments of the gastrointestinal tract of wild‐caught adult Penaeus monodon. Aquaculture Research, 49(1), 378-392. DOI: https://doi.org/10.1111/are.13468
Kolanchinathan, P., Kumari, P. R., Raja, K., John, G., & Balasundaram, A. (2022). Analysis of feed composition and growth parameters of Penaeus monodon supplemented with two probiotic species and formulated diet. Aquaculture, 549, 737740. DOI: https://doi.org/10.1016/j.aquaculture.2021.737740
Hammami, I., Rhouma, A., Jaouadi, B., Rebai, A., & Nesme, X. (2009). Optimization and biochemical characterization of a bacteriocin from a newly isolated Bacillus subtilis strain 14B for biocontrol of Agrobacterium spp. strains. Letters in applied microbiology, 48(2), 253-260. DOI: https://doi.org/10.1111/j.1472-765X.2008.02524.x
Banerjee, G., & Ray, A. K. (2017). The advancement of probiotics research and its application in fish farming industries. Research in veterinary science, 115, 66-77. DOI: https://doi.org/10.1016/j.rvsc.2017.01.016
Delgado, A., López, F. N. A., Brito, D., Peres, C., Fevereiro, P., & Garrido-Fernández, A. (2007). Optimum bacteriocin production by Lactobacillus plantarum 17.2 b requires absence of NaCl and apparently follows a mixed metabolite kinetics. Journal of Biotechnology, 130(2), 193-201. DOI: https://doi.org/10.1016/j.jbiotec.2007.01.041
Martirani, L., Varcamonti, M., Naclerio, G., & De Felice, M. (2002). Purification and partial characterization of bacillocin 490, a novel bacteriocin produced by a thermophilic strain of Bacillus licheniformi s. Microbial cell factories, 1, 1-5. DOI: https://doi.org/10.1186/1475-2859-1-1
Kim, M. H., Kong, Y. J., Baek, H., & Hyun, H. H. (2006). Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5. Journal of biotechnology, 121(1), 54-61.
Amin, A., Khan, M. A., Ehsanullah, M., Haroon, U., Azam, S. M. F., & Hameed, A. (2012). Production of peptide antibiotics by Bacillus sp: GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology, 43, 1340-1346. DOI: https://doi.org/10.1590/S1517-83822012000400015
Todorov, S. D., Nyati, H., Meincken, M., & Dicks, L. M. T. (2007). Partial characterization of bacteriocin AMA-K, produced by Lactobacillus plantarum AMA-K isolated from naturally fermented milk from Zimbabwe. Food control, 18(6), 656-664. DOI: https://doi.org/10.1016/j.foodcont.2006.03.003
Meera, N. S., & Devi, M. C. (2012). Partial characterization and optimization of parameters for Bacteriocin production by Probiotic Lactic acid bacteria. Journal of Microbiology and Biotechnology Research, 2(2), 357-365.
Kim, M. H., Kong, Y. J., Baek, H., & Hyun, H. H. (2006). Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5. Journal of biotechnology, 121(1), 54-61 DOI: https://doi.org/10.1016/j.jbiotec.2005.06.022
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Reena Mol. S
This work is licensed under a Creative Commons Attribution 4.0 International License.
With the licence CC-BY, authors retain the copyright, allowing anyone to download, reuse, re-print, modify, distribute, and/or copy their contribution. The work must be properly attributed to its author.
It is not necessary to ask for further permission from the author or journal board.
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
